Therapeutic effect of human umbilical cord mesenchymal stem cells on neonatal rat hypoxic-ischemic encephalopathy
The therapeutic potential of umbilical cord blood mesenchymal stem cells has been studied in several diseases. However, the possibility that human umbilical cord Wharton's jelly‐derived mesenchymal stem cells (hUCMSCs) can be used to treat neonatal hypoxic–ischemic encephalopathy (HIE) has not...
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| Veröffentlicht in: | Journal of neuroscience research 2014-01, Vol.92 (1), p.35-45 |
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| creator | Zhang, Xinhua Zhang, Qinfen Li, Wei Nie, Dekang Chen, Weiwei Xu, Chunxiang Yi, Xin Shi, Jinhong Tian, Meiling Qin, Jianbing Jin, Guohua Tu, Wenjuan |
| description | The therapeutic potential of umbilical cord blood mesenchymal stem cells has been studied in several diseases. However, the possibility that human umbilical cord Wharton's jelly‐derived mesenchymal stem cells (hUCMSCs) can be used to treat neonatal hypoxic–ischemic encephalopathy (HIE) has not yet been investigated. This study focuses on the potential therapeutic effect of hUCMSC transplantation in a rat model of HIE. Dermal fibroblasts served as cell controls. HIE was induced in neonatal rats aged 7 days. hUCMSCs labeled with Dil were then transplanted into the models 24 hr or 72 hr post‐HIE through the peritoneal cavity or the jugular vein. Behavioral testing revealed that hUCMSC transplantation but not the dermal fibroblast improved significantly the locomotor function vs. vehicle controls. Animals receiving cell grafts 24 hr after surgery showed a more significant improvement than at 72 hr. More hUCMSCs homed to the ischemic frontal cortex following intravenous administration than after intraperitoneal injection. Differentiation of engrafted cells into neurons was observed in and around the infarct region. Gliosis in ischemic regions was significantly reduced after hUCMSC transplantation. Administration of ganglioside (GM1) enhanced the behavioral recovery on the base of hUCMSC treatment. These results demonstrate that intravenous transplantation of hUCMSCs at an early stage after HIE can improve the behavior of hypoxic–ischemic rats and decrease gliosis. Ganglioside treatment further enhanced the recovery of neurological function following hUCMSC transplantation. © 2013 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/jnr.23304 |
| format | Article |
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However, the possibility that human umbilical cord Wharton's jelly‐derived mesenchymal stem cells (hUCMSCs) can be used to treat neonatal hypoxic–ischemic encephalopathy (HIE) has not yet been investigated. This study focuses on the potential therapeutic effect of hUCMSC transplantation in a rat model of HIE. Dermal fibroblasts served as cell controls. HIE was induced in neonatal rats aged 7 days. hUCMSCs labeled with Dil were then transplanted into the models 24 hr or 72 hr post‐HIE through the peritoneal cavity or the jugular vein. Behavioral testing revealed that hUCMSC transplantation but not the dermal fibroblast improved significantly the locomotor function vs. vehicle controls. Animals receiving cell grafts 24 hr after surgery showed a more significant improvement than at 72 hr. More hUCMSCs homed to the ischemic frontal cortex following intravenous administration than after intraperitoneal injection. Differentiation of engrafted cells into neurons was observed in and around the infarct region. Gliosis in ischemic regions was significantly reduced after hUCMSC transplantation. Administration of ganglioside (GM1) enhanced the behavioral recovery on the base of hUCMSC treatment. These results demonstrate that intravenous transplantation of hUCMSCs at an early stage after HIE can improve the behavior of hypoxic–ischemic rats and decrease gliosis. Ganglioside treatment further enhanced the recovery of neurological function following hUCMSC transplantation. © 2013 Wiley Periodicals, Inc.</description><identifier>ISSN: 0360-4012</identifier><identifier>EISSN: 1097-4547</identifier><identifier>DOI: 10.1002/jnr.23304</identifier><identifier>PMID: 24265136</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Animal models ; Animals ; Cell Differentiation - physiology ; ganglioside ; human umbilical cord mesenchymal stem cells ; Humans ; Hypoxia-Ischemia, Brain - physiopathology ; Hypoxia-Ischemia, Brain - therapy ; hypoxic-ischemic encephalopathy ; Maze Learning - physiology ; Mesenchymal Stem Cell Transplantation ; neonatal rats ; Neurons - cytology ; Rats ; Rats, Sprague-Dawley ; Rotarod Performance Test ; Umbilical Cord - cytology</subject><ispartof>Journal of neuroscience research, 2014-01, Vol.92 (1), p.35-45</ispartof><rights>Copyright © 2013 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4904-93ea2fe559014c0bd961bb9be2e0f9a9ca00c8a2fde168f15d22ca473be451043</citedby><cites>FETCH-LOGICAL-c4904-93ea2fe559014c0bd961bb9be2e0f9a9ca00c8a2fde168f15d22ca473be451043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjnr.23304$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjnr.23304$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27907,27908,45557,45558</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24265136$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Xinhua</creatorcontrib><creatorcontrib>Zhang, Qinfen</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><creatorcontrib>Nie, Dekang</creatorcontrib><creatorcontrib>Chen, Weiwei</creatorcontrib><creatorcontrib>Xu, Chunxiang</creatorcontrib><creatorcontrib>Yi, Xin</creatorcontrib><creatorcontrib>Shi, Jinhong</creatorcontrib><creatorcontrib>Tian, Meiling</creatorcontrib><creatorcontrib>Qin, Jianbing</creatorcontrib><creatorcontrib>Jin, Guohua</creatorcontrib><creatorcontrib>Tu, Wenjuan</creatorcontrib><title>Therapeutic effect of human umbilical cord mesenchymal stem cells on neonatal rat hypoxic-ischemic encephalopathy</title><title>Journal of neuroscience research</title><addtitle>Journal of Neuroscience Research</addtitle><description>The therapeutic potential of umbilical cord blood mesenchymal stem cells has been studied in several diseases. However, the possibility that human umbilical cord Wharton's jelly‐derived mesenchymal stem cells (hUCMSCs) can be used to treat neonatal hypoxic–ischemic encephalopathy (HIE) has not yet been investigated. This study focuses on the potential therapeutic effect of hUCMSC transplantation in a rat model of HIE. Dermal fibroblasts served as cell controls. HIE was induced in neonatal rats aged 7 days. hUCMSCs labeled with Dil were then transplanted into the models 24 hr or 72 hr post‐HIE through the peritoneal cavity or the jugular vein. Behavioral testing revealed that hUCMSC transplantation but not the dermal fibroblast improved significantly the locomotor function vs. vehicle controls. Animals receiving cell grafts 24 hr after surgery showed a more significant improvement than at 72 hr. More hUCMSCs homed to the ischemic frontal cortex following intravenous administration than after intraperitoneal injection. Differentiation of engrafted cells into neurons was observed in and around the infarct region. Gliosis in ischemic regions was significantly reduced after hUCMSC transplantation. Administration of ganglioside (GM1) enhanced the behavioral recovery on the base of hUCMSC treatment. These results demonstrate that intravenous transplantation of hUCMSCs at an early stage after HIE can improve the behavior of hypoxic–ischemic rats and decrease gliosis. Ganglioside treatment further enhanced the recovery of neurological function following hUCMSC transplantation. © 2013 Wiley Periodicals, Inc.</description><subject>Animal models</subject><subject>Animals</subject><subject>Cell Differentiation - physiology</subject><subject>ganglioside</subject><subject>human umbilical cord mesenchymal stem cells</subject><subject>Humans</subject><subject>Hypoxia-Ischemia, Brain - physiopathology</subject><subject>Hypoxia-Ischemia, Brain - therapy</subject><subject>hypoxic-ischemic encephalopathy</subject><subject>Maze Learning - physiology</subject><subject>Mesenchymal Stem Cell Transplantation</subject><subject>neonatal rats</subject><subject>Neurons - cytology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Rotarod Performance Test</subject><subject>Umbilical Cord - cytology</subject><issn>0360-4012</issn><issn>1097-4547</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EokvhwB9AlrjAIe34K1kfUQULVbWt0CKOluNMlCxxktqJaP49XrbtoRISJ0ujZ16_o4eQtwzOGAA_3_fhjAsB8hlZMdBFJpUsnpMViBwyCYyfkFcx7gFAayVekhMuea6YyFfkdtdgsCPOU-so1jW6iQ41bWZvezr7su1aZzvqhlBRjxF71yw-DeKEnjrsukiHnvY49HZK42An2izjcNe6rI2uQX-I7R2Oje2G0U7N8pq8qG0X8c39e0p-fPm8u_iaXV1vvl18usqc1CAzLdDyGpXSwKSDstI5K0tdIkeotdXOArh1Qipk-bpmquLcWVmIEqViIMUp-XDMHcNwO2OcjE-NUmOb2s7RMJmvhVKg1v-DMiG5YDyh75-g-2EOfTrkQAHLU-HD3x-PlAtDjAFrM4bW27AYBuagzCRl5q-yxL67T5xLj9Uj-eAoAedH4Hfb4fLvJHO5_f4QmR032qTp7nHDhl8mL0ShzM_txuQbofT2ZmeU-ANNFK--</recordid><startdate>201401</startdate><enddate>201401</enddate><creator>Zhang, Xinhua</creator><creator>Zhang, Qinfen</creator><creator>Li, Wei</creator><creator>Nie, Dekang</creator><creator>Chen, Weiwei</creator><creator>Xu, Chunxiang</creator><creator>Yi, Xin</creator><creator>Shi, Jinhong</creator><creator>Tian, Meiling</creator><creator>Qin, Jianbing</creator><creator>Jin, Guohua</creator><creator>Tu, Wenjuan</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201401</creationdate><title>Therapeutic effect of human umbilical cord mesenchymal stem cells on neonatal rat hypoxic-ischemic encephalopathy</title><author>Zhang, Xinhua ; Zhang, Qinfen ; Li, Wei ; Nie, Dekang ; Chen, Weiwei ; Xu, Chunxiang ; Yi, Xin ; Shi, Jinhong ; Tian, Meiling ; Qin, Jianbing ; Jin, Guohua ; Tu, Wenjuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4904-93ea2fe559014c0bd961bb9be2e0f9a9ca00c8a2fde168f15d22ca473be451043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animal models</topic><topic>Animals</topic><topic>Cell Differentiation - physiology</topic><topic>ganglioside</topic><topic>human umbilical cord mesenchymal stem cells</topic><topic>Humans</topic><topic>Hypoxia-Ischemia, Brain - physiopathology</topic><topic>Hypoxia-Ischemia, Brain - therapy</topic><topic>hypoxic-ischemic encephalopathy</topic><topic>Maze Learning - physiology</topic><topic>Mesenchymal Stem Cell Transplantation</topic><topic>neonatal rats</topic><topic>Neurons - cytology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Rotarod Performance Test</topic><topic>Umbilical Cord - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Xinhua</creatorcontrib><creatorcontrib>Zhang, Qinfen</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><creatorcontrib>Nie, Dekang</creatorcontrib><creatorcontrib>Chen, Weiwei</creatorcontrib><creatorcontrib>Xu, Chunxiang</creatorcontrib><creatorcontrib>Yi, Xin</creatorcontrib><creatorcontrib>Shi, Jinhong</creatorcontrib><creatorcontrib>Tian, Meiling</creatorcontrib><creatorcontrib>Qin, Jianbing</creatorcontrib><creatorcontrib>Jin, Guohua</creatorcontrib><creatorcontrib>Tu, Wenjuan</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroscience research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Xinhua</au><au>Zhang, Qinfen</au><au>Li, Wei</au><au>Nie, Dekang</au><au>Chen, Weiwei</au><au>Xu, Chunxiang</au><au>Yi, Xin</au><au>Shi, Jinhong</au><au>Tian, Meiling</au><au>Qin, Jianbing</au><au>Jin, Guohua</au><au>Tu, Wenjuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Therapeutic effect of human umbilical cord mesenchymal stem cells on neonatal rat hypoxic-ischemic encephalopathy</atitle><jtitle>Journal of neuroscience research</jtitle><addtitle>Journal of Neuroscience Research</addtitle><date>2014-01</date><risdate>2014</risdate><volume>92</volume><issue>1</issue><spage>35</spage><epage>45</epage><pages>35-45</pages><issn>0360-4012</issn><eissn>1097-4547</eissn><abstract>The therapeutic potential of umbilical cord blood mesenchymal stem cells has been studied in several diseases. However, the possibility that human umbilical cord Wharton's jelly‐derived mesenchymal stem cells (hUCMSCs) can be used to treat neonatal hypoxic–ischemic encephalopathy (HIE) has not yet been investigated. This study focuses on the potential therapeutic effect of hUCMSC transplantation in a rat model of HIE. Dermal fibroblasts served as cell controls. HIE was induced in neonatal rats aged 7 days. hUCMSCs labeled with Dil were then transplanted into the models 24 hr or 72 hr post‐HIE through the peritoneal cavity or the jugular vein. Behavioral testing revealed that hUCMSC transplantation but not the dermal fibroblast improved significantly the locomotor function vs. vehicle controls. Animals receiving cell grafts 24 hr after surgery showed a more significant improvement than at 72 hr. More hUCMSCs homed to the ischemic frontal cortex following intravenous administration than after intraperitoneal injection. Differentiation of engrafted cells into neurons was observed in and around the infarct region. Gliosis in ischemic regions was significantly reduced after hUCMSC transplantation. Administration of ganglioside (GM1) enhanced the behavioral recovery on the base of hUCMSC treatment. These results demonstrate that intravenous transplantation of hUCMSCs at an early stage after HIE can improve the behavior of hypoxic–ischemic rats and decrease gliosis. Ganglioside treatment further enhanced the recovery of neurological function following hUCMSC transplantation. © 2013 Wiley Periodicals, Inc.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>24265136</pmid><doi>10.1002/jnr.23304</doi><tpages>11</tpages></addata></record> |
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| subjects | Animal models Animals Cell Differentiation - physiology ganglioside human umbilical cord mesenchymal stem cells Humans Hypoxia-Ischemia, Brain - physiopathology Hypoxia-Ischemia, Brain - therapy hypoxic-ischemic encephalopathy Maze Learning - physiology Mesenchymal Stem Cell Transplantation neonatal rats Neurons - cytology Rats Rats, Sprague-Dawley Rotarod Performance Test Umbilical Cord - cytology |
| title | Therapeutic effect of human umbilical cord mesenchymal stem cells on neonatal rat hypoxic-ischemic encephalopathy |
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