Alternative Fusion Protein Strategies to Express Recalcitrant QconCAT Proteins for Quantitative Proteomics of Human Drug Metabolizing Enzymes and Transporters

QconCAT is a tool for quantitative proteomics, consisting of an artificial protein, expressed from an artificial gene, made up of a concatenated string of proteotypic peptides selected from the proteins under study. Isotopically labeled QconCAT (usually containing 13C6-arginine and 13C6-lysine) prov...

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Veröffentlicht in:	Journal of proteome research 2013-12, Vol.12 (12), p.5934-5942
Hauptverfasser:	Russell, Matthew R, Achour, Brahim, Mckenzie, Edward A, Lopez, Ruth, Harwood, Matthew D, Rostami-Hodjegan, Amin, Barber, Jill
Format:	Artikel
Sprache:	eng
Schlagworte:	Caco-2 Cells Carbon Isotopes Cell Membrane - metabolism Escherichia coli - genetics Escherichia coli - metabolism Gene Expression Regulation Genes, Synthetic Genetic Engineering Genetic Vectors - metabolism Humans Inactivation, Metabolic - genetics Liver - chemistry Liver - enzymology Molecular Chaperones - genetics Molecular Chaperones - metabolism Peptides - metabolism Polylysine - metabolism Protein Transport Proteomics Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism
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