New trends in LC protein ligand screening
•A critical view of bioaffinity-based strategies for protein ligand screening.•Zonal, frontal and nonlinear chromatography with immobilized proteins is presented.•Ligand fishing with magnetic beads is an innovative tool also discussed. The diversity of small molecules available to produce truly inno...
Gespeichert in:
| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2014-01, Vol.87, p.155-166 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 166 |
|---|---|
| container_issue | |
| container_start_page | 155 |
| container_title | Journal of pharmaceutical and biomedical analysis |
| container_volume | 87 |
| creator | de Moraes, Marcela C. Vanzolini, Kenia L. Cardoso, Carmen L. Cass, Quezia B. |
| description | •A critical view of bioaffinity-based strategies for protein ligand screening.•Zonal, frontal and nonlinear chromatography with immobilized proteins is presented.•Ligand fishing with magnetic beads is an innovative tool also discussed.
The diversity of small molecules available to produce truly innovative drugs associated with the wealth of known biological targets calls for key strategies in protein ligand screening. This review encompasses the recently developed bioaffinity-based strategies. A critical view of the use of zonal, frontal, and nonlinear chromatography with immobilized proteins is given. The association of these elution modes with the ligand fishing method, which uses nanomagnetics particles, is also addressed. A series of applications and how these new screening strategies can be used to determine the function, affinity, and activity parameters of proteins is discussed. |
| doi_str_mv | 10.1016/j.jpba.2013.07.021 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1466375969</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0731708513003270</els_id><sourcerecordid>1466375969</sourcerecordid><originalsourceid>FETCH-LOGICAL-c356t-cc47a86cd2ed34e087a4b691cf2524cc4cc52299122b122fcc68ddcc21a4c8893</originalsourceid><addsrcrecordid>eNp9kE1Lw0AQhhdRbK3-AQ-Sox4S9yu7CXiR4hcUvSh4Wzazk7IhTetuqvjvTWj16GGYgXnfl5mHkHNGM0aZum6yZlPZjFMmMqozytkBmbJCi5Qr-X5IplQLlmpa5BNyEmNDKc1ZKY_JhIsyF5rmU3L1jF9JH7BzMfFdspgnm7DucRhbv7SdSyIExM53y1NyVNs24tm-z8jb_d3r_DFdvDw8zW8XKYhc9SmA1LZQ4Dg6IZEW2spKlQxqnnM5bAFyzsuScV4NVQOowjkAzqyEoijFjFzucodDPrYYe7PyEbBtbYfrbTRMKiV0XqpRyndSCOsYA9ZmE_zKhm_DqBkRmcaMiMyIyFBtBkSD6WKfv61W6P4sv0wGwc1OgMOXnx6DieCxA3Q-IPTGrf1_-T_BLXX4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1466375969</pqid></control><display><type>article</type><title>New trends in LC protein ligand screening</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>de Moraes, Marcela C. ; Vanzolini, Kenia L. ; Cardoso, Carmen L. ; Cass, Quezia B.</creator><creatorcontrib>de Moraes, Marcela C. ; Vanzolini, Kenia L. ; Cardoso, Carmen L. ; Cass, Quezia B.</creatorcontrib><description>•A critical view of bioaffinity-based strategies for protein ligand screening.•Zonal, frontal and nonlinear chromatography with immobilized proteins is presented.•Ligand fishing with magnetic beads is an innovative tool also discussed.
The diversity of small molecules available to produce truly innovative drugs associated with the wealth of known biological targets calls for key strategies in protein ligand screening. This review encompasses the recently developed bioaffinity-based strategies. A critical view of the use of zonal, frontal, and nonlinear chromatography with immobilized proteins is given. The association of these elution modes with the ligand fishing method, which uses nanomagnetics particles, is also addressed. A series of applications and how these new screening strategies can be used to determine the function, affinity, and activity parameters of proteins is discussed.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2013.07.021</identifier><identifier>PMID: 23953705</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Bioaffinity-based strategies ; Chromatography, Affinity - methods ; Frontal chromatography ; Humans ; Immobilized Proteins - chemistry ; Ligands ; Magnetics ; Nanoparticles ; Nonlinear chromatography ; Protein Binding ; Protein ligand screening ; Proteins - chemistry ; Zonal chromatography</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2014-01, Vol.87, p.155-166</ispartof><rights>2013 Elsevier B.V.</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-cc47a86cd2ed34e087a4b691cf2524cc4cc52299122b122fcc68ddcc21a4c8893</citedby><cites>FETCH-LOGICAL-c356t-cc47a86cd2ed34e087a4b691cf2524cc4cc52299122b122fcc68ddcc21a4c8893</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0731708513003270$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23953705$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Moraes, Marcela C.</creatorcontrib><creatorcontrib>Vanzolini, Kenia L.</creatorcontrib><creatorcontrib>Cardoso, Carmen L.</creatorcontrib><creatorcontrib>Cass, Quezia B.</creatorcontrib><title>New trends in LC protein ligand screening</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>•A critical view of bioaffinity-based strategies for protein ligand screening.•Zonal, frontal and nonlinear chromatography with immobilized proteins is presented.•Ligand fishing with magnetic beads is an innovative tool also discussed.
The diversity of small molecules available to produce truly innovative drugs associated with the wealth of known biological targets calls for key strategies in protein ligand screening. This review encompasses the recently developed bioaffinity-based strategies. A critical view of the use of zonal, frontal, and nonlinear chromatography with immobilized proteins is given. The association of these elution modes with the ligand fishing method, which uses nanomagnetics particles, is also addressed. A series of applications and how these new screening strategies can be used to determine the function, affinity, and activity parameters of proteins is discussed.</description><subject>Bioaffinity-based strategies</subject><subject>Chromatography, Affinity - methods</subject><subject>Frontal chromatography</subject><subject>Humans</subject><subject>Immobilized Proteins - chemistry</subject><subject>Ligands</subject><subject>Magnetics</subject><subject>Nanoparticles</subject><subject>Nonlinear chromatography</subject><subject>Protein Binding</subject><subject>Protein ligand screening</subject><subject>Proteins - chemistry</subject><subject>Zonal chromatography</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1Lw0AQhhdRbK3-AQ-Sox4S9yu7CXiR4hcUvSh4Wzazk7IhTetuqvjvTWj16GGYgXnfl5mHkHNGM0aZum6yZlPZjFMmMqozytkBmbJCi5Qr-X5IplQLlmpa5BNyEmNDKc1ZKY_JhIsyF5rmU3L1jF9JH7BzMfFdspgnm7DucRhbv7SdSyIExM53y1NyVNs24tm-z8jb_d3r_DFdvDw8zW8XKYhc9SmA1LZQ4Dg6IZEW2spKlQxqnnM5bAFyzsuScV4NVQOowjkAzqyEoijFjFzucodDPrYYe7PyEbBtbYfrbTRMKiV0XqpRyndSCOsYA9ZmE_zKhm_DqBkRmcaMiMyIyFBtBkSD6WKfv61W6P4sv0wGwc1OgMOXnx6DieCxA3Q-IPTGrf1_-T_BLXX4</recordid><startdate>20140118</startdate><enddate>20140118</enddate><creator>de Moraes, Marcela C.</creator><creator>Vanzolini, Kenia L.</creator><creator>Cardoso, Carmen L.</creator><creator>Cass, Quezia B.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140118</creationdate><title>New trends in LC protein ligand screening</title><author>de Moraes, Marcela C. ; Vanzolini, Kenia L. ; Cardoso, Carmen L. ; Cass, Quezia B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-cc47a86cd2ed34e087a4b691cf2524cc4cc52299122b122fcc68ddcc21a4c8893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Bioaffinity-based strategies</topic><topic>Chromatography, Affinity - methods</topic><topic>Frontal chromatography</topic><topic>Humans</topic><topic>Immobilized Proteins - chemistry</topic><topic>Ligands</topic><topic>Magnetics</topic><topic>Nanoparticles</topic><topic>Nonlinear chromatography</topic><topic>Protein Binding</topic><topic>Protein ligand screening</topic><topic>Proteins - chemistry</topic><topic>Zonal chromatography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Moraes, Marcela C.</creatorcontrib><creatorcontrib>Vanzolini, Kenia L.</creatorcontrib><creatorcontrib>Cardoso, Carmen L.</creatorcontrib><creatorcontrib>Cass, Quezia B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Moraes, Marcela C.</au><au>Vanzolini, Kenia L.</au><au>Cardoso, Carmen L.</au><au>Cass, Quezia B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New trends in LC protein ligand screening</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2014-01-18</date><risdate>2014</risdate><volume>87</volume><spage>155</spage><epage>166</epage><pages>155-166</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><abstract>•A critical view of bioaffinity-based strategies for protein ligand screening.•Zonal, frontal and nonlinear chromatography with immobilized proteins is presented.•Ligand fishing with magnetic beads is an innovative tool also discussed.
The diversity of small molecules available to produce truly innovative drugs associated with the wealth of known biological targets calls for key strategies in protein ligand screening. This review encompasses the recently developed bioaffinity-based strategies. A critical view of the use of zonal, frontal, and nonlinear chromatography with immobilized proteins is given. The association of these elution modes with the ligand fishing method, which uses nanomagnetics particles, is also addressed. A series of applications and how these new screening strategies can be used to determine the function, affinity, and activity parameters of proteins is discussed.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>23953705</pmid><doi>10.1016/j.jpba.2013.07.021</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0731-7085 |
| ispartof | Journal of pharmaceutical and biomedical analysis, 2014-01, Vol.87, p.155-166 |
| issn | 0731-7085 1873-264X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1466375969 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Bioaffinity-based strategies Chromatography, Affinity - methods Frontal chromatography Humans Immobilized Proteins - chemistry Ligands Magnetics Nanoparticles Nonlinear chromatography Protein Binding Protein ligand screening Proteins - chemistry Zonal chromatography |
| title | New trends in LC protein ligand screening |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T02%3A34%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=New%20trends%20in%20LC%20protein%20ligand%20screening&rft.jtitle=Journal%20of%20pharmaceutical%20and%20biomedical%20analysis&rft.au=de%20Moraes,%20Marcela%20C.&rft.date=2014-01-18&rft.volume=87&rft.spage=155&rft.epage=166&rft.pages=155-166&rft.issn=0731-7085&rft.eissn=1873-264X&rft_id=info:doi/10.1016/j.jpba.2013.07.021&rft_dat=%3Cproquest_cross%3E1466375969%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1466375969&rft_id=info:pmid/23953705&rft_els_id=S0731708513003270&rfr_iscdi=true |