Copper-Assisted Click Reactions for Activity-Based Proteomics: Fine-Tuned Ligands and Refined Conditions Extend the Scope of Application

Copper‐catalysed alkyne–azide 1,3‐dipolar cycloaddition (CuAAC) is the predominantly used bioconjugation method in the field of activity‐based protein profiling (ABPP). Several limitations, however, including conversion efficiency, protein denaturation and buffer compatibility, restrict the scope of...

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Veröffentlicht in:	Chembiochem : a European journal of chemical biology 2013-12, Vol.14 (18), p.2447-2455
Hauptverfasser:	Rudolf, Georg C., Sieber, Stephan A.
Format:	Artikel
Sprache:	eng
Schlagworte:	ABPP Alkynes - chemistry analytical methods Azides - chemistry click chemistry Click Chemistry - methods Copper Copper - chemistry CuAAC Cycloaddition Reaction Ligands Proteomics Proteomics - methods Triazoles - chemistry Trifluoroacetic Acid - chemistry
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