Time-Resolved in-Situ Observation of Starch Polysaccharide Degradation Pathways

Analytical challenges in the direct time‐resolved observation of starch metabolism have been addressed by using optimized multidimensional NMR experiments. Starch provides the main source of human dietary energy intake and is a raw material for beverage and renewable fuel production. Use of direct i...

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Veröffentlicht in:Chembiochem : a European journal of chemical biology 2013-12, Vol.14 (18), p.2506-2511
Hauptverfasser: Beeren, Sophie R., Petersen, Bent O., Bøjstrup, Marie, Hindsgaul, Ole, Meier, Sebastian
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container_issue 18
container_start_page 2506
container_title Chembiochem : a European journal of chemical biology
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creator Beeren, Sophie R.
Petersen, Bent O.
Bøjstrup, Marie
Hindsgaul, Ole
Meier, Sebastian
description Analytical challenges in the direct time‐resolved observation of starch metabolism have been addressed by using optimized multidimensional NMR experiments. Starch provides the main source of human dietary energy intake and is a raw material for beverage and renewable fuel production. Use of direct in situ observations of starch remodeling pathways could facilitate our understanding and control of processes of biotechnological, medical, and environmental relevance. Processes involving starch synthesis or degradation are difficult to monitor directly in aqueous solution, however, because starch consists of glucopyranosyl homopolymers that are built up from and degraded into structurally similar fragments that yield only small signal dispersion in optical and NMR spectroscopy. By focusing on acetal groups only, 1H,13C HSQC experiments sampling narrow spectral windows in the highly resolved 13C dimension have been employed in order to observe the amylopectin cleavage pathway in real time with a temporal resolution of 150 s. Quantifiable signals for more than 15 molecular species emerging during starch fragmentation by human saliva have been resolved and tracked over time in this manner. Altered accumulation of intermediates in the digestion of amylopectin in the presence of black tea acting as an effector have been monitored. Direct observations of starch remodeling should facilitate our understanding and control of processes of biotechnological, medical, and environmental relevance. Here, analytical challenges in time‐resolved in situ observation of starch metabolism are addressed by means of resolution‐optimized multidimensional NMR experiments.
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Starch provides the main source of human dietary energy intake and is a raw material for beverage and renewable fuel production. Use of direct in situ observations of starch remodeling pathways could facilitate our understanding and control of processes of biotechnological, medical, and environmental relevance. Processes involving starch synthesis or degradation are difficult to monitor directly in aqueous solution, however, because starch consists of glucopyranosyl homopolymers that are built up from and degraded into structurally similar fragments that yield only small signal dispersion in optical and NMR spectroscopy. By focusing on acetal groups only, 1H,13C HSQC experiments sampling narrow spectral windows in the highly resolved 13C dimension have been employed in order to observe the amylopectin cleavage pathway in real time with a temporal resolution of 150 s. Quantifiable signals for more than 15 molecular species emerging during starch fragmentation by human saliva have been resolved and tracked over time in this manner. Altered accumulation of intermediates in the digestion of amylopectin in the presence of black tea acting as an effector have been monitored. Direct observations of starch remodeling should facilitate our understanding and control of processes of biotechnological, medical, and environmental relevance. 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subjects amylases
Amylopectin - analysis
Amylopectin - metabolism
Aqueous solutions
carbohydrates
Humans
Magnetic Resonance Spectroscopy - methods
NMR
NMR spectroscopy
Nuclear magnetic resonance
pathway intermediates
Polysaccharides - analysis
Polysaccharides - metabolism
Saliva - chemistry
Saliva - metabolism
Starch - analysis
Starch - metabolism
title Time-Resolved in-Situ Observation of Starch Polysaccharide Degradation Pathways
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