Time-Resolved in-Situ Observation of Starch Polysaccharide Degradation Pathways
Analytical challenges in the direct time‐resolved observation of starch metabolism have been addressed by using optimized multidimensional NMR experiments. Starch provides the main source of human dietary energy intake and is a raw material for beverage and renewable fuel production. Use of direct i...
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Veröffentlicht in: | Chembiochem : a European journal of chemical biology 2013-12, Vol.14 (18), p.2506-2511 |
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creator | Beeren, Sophie R. Petersen, Bent O. Bøjstrup, Marie Hindsgaul, Ole Meier, Sebastian |
description | Analytical challenges in the direct time‐resolved observation of starch metabolism have been addressed by using optimized multidimensional NMR experiments. Starch provides the main source of human dietary energy intake and is a raw material for beverage and renewable fuel production. Use of direct in situ observations of starch remodeling pathways could facilitate our understanding and control of processes of biotechnological, medical, and environmental relevance. Processes involving starch synthesis or degradation are difficult to monitor directly in aqueous solution, however, because starch consists of glucopyranosyl homopolymers that are built up from and degraded into structurally similar fragments that yield only small signal dispersion in optical and NMR spectroscopy. By focusing on acetal groups only, 1H,13C HSQC experiments sampling narrow spectral windows in the highly resolved 13C dimension have been employed in order to observe the amylopectin cleavage pathway in real time with a temporal resolution of 150 s. Quantifiable signals for more than 15 molecular species emerging during starch fragmentation by human saliva have been resolved and tracked over time in this manner. Altered accumulation of intermediates in the digestion of amylopectin in the presence of black tea acting as an effector have been monitored.
Direct observations of starch remodeling should facilitate our understanding and control of processes of biotechnological, medical, and environmental relevance. Here, analytical challenges in time‐resolved in situ observation of starch metabolism are addressed by means of resolution‐optimized multidimensional NMR experiments. |
doi_str_mv | 10.1002/cbic.201300461 |
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Direct observations of starch remodeling should facilitate our understanding and control of processes of biotechnological, medical, and environmental relevance. Here, analytical challenges in time‐resolved in situ observation of starch metabolism are addressed by means of resolution‐optimized multidimensional NMR experiments.</description><identifier>ISSN: 1439-4227</identifier><identifier>EISSN: 1439-7633</identifier><identifier>DOI: 10.1002/cbic.201300461</identifier><identifier>PMID: 24166781</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>amylases ; Amylopectin - analysis ; Amylopectin - metabolism ; Aqueous solutions ; carbohydrates ; Humans ; Magnetic Resonance Spectroscopy - methods ; NMR ; NMR spectroscopy ; Nuclear magnetic resonance ; pathway intermediates ; Polysaccharides - analysis ; Polysaccharides - metabolism ; Saliva - chemistry ; Saliva - metabolism ; Starch - analysis ; Starch - metabolism</subject><ispartof>Chembiochem : a European journal of chemical biology, 2013-12, Vol.14 (18), p.2506-2511</ispartof><rights>Copyright © 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4481-9f09e602cef47c459dd6074537d2296e6511fed8d50971ed96029afdea5c84bf3</citedby><cites>FETCH-LOGICAL-c4481-9f09e602cef47c459dd6074537d2296e6511fed8d50971ed96029afdea5c84bf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbic.201300461$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbic.201300461$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24166781$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Beeren, Sophie R.</creatorcontrib><creatorcontrib>Petersen, Bent O.</creatorcontrib><creatorcontrib>Bøjstrup, Marie</creatorcontrib><creatorcontrib>Hindsgaul, Ole</creatorcontrib><creatorcontrib>Meier, Sebastian</creatorcontrib><title>Time-Resolved in-Situ Observation of Starch Polysaccharide Degradation Pathways</title><title>Chembiochem : a European journal of chemical biology</title><addtitle>ChemBioChem</addtitle><description>Analytical challenges in the direct time‐resolved observation of starch metabolism have been addressed by using optimized multidimensional NMR experiments. Starch provides the main source of human dietary energy intake and is a raw material for beverage and renewable fuel production. Use of direct in situ observations of starch remodeling pathways could facilitate our understanding and control of processes of biotechnological, medical, and environmental relevance. Processes involving starch synthesis or degradation are difficult to monitor directly in aqueous solution, however, because starch consists of glucopyranosyl homopolymers that are built up from and degraded into structurally similar fragments that yield only small signal dispersion in optical and NMR spectroscopy. By focusing on acetal groups only, 1H,13C HSQC experiments sampling narrow spectral windows in the highly resolved 13C dimension have been employed in order to observe the amylopectin cleavage pathway in real time with a temporal resolution of 150 s. Quantifiable signals for more than 15 molecular species emerging during starch fragmentation by human saliva have been resolved and tracked over time in this manner. Altered accumulation of intermediates in the digestion of amylopectin in the presence of black tea acting as an effector have been monitored.
Direct observations of starch remodeling should facilitate our understanding and control of processes of biotechnological, medical, and environmental relevance. Here, analytical challenges in time‐resolved in situ observation of starch metabolism are addressed by means of resolution‐optimized multidimensional NMR experiments.</description><subject>amylases</subject><subject>Amylopectin - analysis</subject><subject>Amylopectin - metabolism</subject><subject>Aqueous solutions</subject><subject>carbohydrates</subject><subject>Humans</subject><subject>Magnetic Resonance Spectroscopy - methods</subject><subject>NMR</subject><subject>NMR spectroscopy</subject><subject>Nuclear magnetic resonance</subject><subject>pathway intermediates</subject><subject>Polysaccharides - analysis</subject><subject>Polysaccharides - metabolism</subject><subject>Saliva - chemistry</subject><subject>Saliva - metabolism</subject><subject>Starch - analysis</subject><subject>Starch - metabolism</subject><issn>1439-4227</issn><issn>1439-7633</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0D1PwzAQBmALgfheGVEkFpYUO3bseIQABQloRUGMlmtfqCFtwE6A_ntSpVSIhck3PPfK9yJ0QHCPYJycmLEzvQQTijHjZA1tE0ZlLDil68uZJYnYQjshvGCMJadkE20ljHAuMrKNBg9uCvE9hKr8ABu5WTxydRMNxgH8h65dNYuqIhrV2ptJNKzKedDGTLR3FqJzePbadmio68mnnoc9tFHoMsD-8t1Fj5cXD_lVfDPoX-enN7FhLCOxLLAEjhMDBROGpdJajgVLqbBJIjnwlJACbGZTLAUBK1srdWFBpyZj44LuouMu981X7w2EWk1dMFCWegZVExRhnFORtne29OgPfakaP2t_t1BpxrhMRat6nTK-CsFDod68m2o_VwSrRdVqUbVaVd0uHC5jm_EU7Ir_dNsC2YFPV8L8nziVn13nv8PjbteFGr5Wu9q_Ki7au9TTXV_R4e0oI3lfPdFvHP2YuA</recordid><startdate>20131216</startdate><enddate>20131216</enddate><creator>Beeren, Sophie R.</creator><creator>Petersen, Bent O.</creator><creator>Bøjstrup, Marie</creator><creator>Hindsgaul, Ole</creator><creator>Meier, Sebastian</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20131216</creationdate><title>Time-Resolved in-Situ Observation of Starch Polysaccharide Degradation Pathways</title><author>Beeren, Sophie R. ; Petersen, Bent O. ; Bøjstrup, Marie ; Hindsgaul, Ole ; Meier, Sebastian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4481-9f09e602cef47c459dd6074537d2296e6511fed8d50971ed96029afdea5c84bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>amylases</topic><topic>Amylopectin - analysis</topic><topic>Amylopectin - metabolism</topic><topic>Aqueous solutions</topic><topic>carbohydrates</topic><topic>Humans</topic><topic>Magnetic Resonance Spectroscopy - methods</topic><topic>NMR</topic><topic>NMR spectroscopy</topic><topic>Nuclear magnetic resonance</topic><topic>pathway intermediates</topic><topic>Polysaccharides - analysis</topic><topic>Polysaccharides - metabolism</topic><topic>Saliva - chemistry</topic><topic>Saliva - metabolism</topic><topic>Starch - analysis</topic><topic>Starch - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Beeren, Sophie R.</creatorcontrib><creatorcontrib>Petersen, Bent O.</creatorcontrib><creatorcontrib>Bøjstrup, Marie</creatorcontrib><creatorcontrib>Hindsgaul, Ole</creatorcontrib><creatorcontrib>Meier, Sebastian</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Chembiochem : a European journal of chemical biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Beeren, Sophie R.</au><au>Petersen, Bent O.</au><au>Bøjstrup, Marie</au><au>Hindsgaul, Ole</au><au>Meier, Sebastian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Time-Resolved in-Situ Observation of Starch Polysaccharide Degradation Pathways</atitle><jtitle>Chembiochem : a European journal of chemical biology</jtitle><addtitle>ChemBioChem</addtitle><date>2013-12-16</date><risdate>2013</risdate><volume>14</volume><issue>18</issue><spage>2506</spage><epage>2511</epage><pages>2506-2511</pages><issn>1439-4227</issn><eissn>1439-7633</eissn><abstract>Analytical challenges in the direct time‐resolved observation of starch metabolism have been addressed by using optimized multidimensional NMR experiments. Starch provides the main source of human dietary energy intake and is a raw material for beverage and renewable fuel production. Use of direct in situ observations of starch remodeling pathways could facilitate our understanding and control of processes of biotechnological, medical, and environmental relevance. Processes involving starch synthesis or degradation are difficult to monitor directly in aqueous solution, however, because starch consists of glucopyranosyl homopolymers that are built up from and degraded into structurally similar fragments that yield only small signal dispersion in optical and NMR spectroscopy. By focusing on acetal groups only, 1H,13C HSQC experiments sampling narrow spectral windows in the highly resolved 13C dimension have been employed in order to observe the amylopectin cleavage pathway in real time with a temporal resolution of 150 s. Quantifiable signals for more than 15 molecular species emerging during starch fragmentation by human saliva have been resolved and tracked over time in this manner. Altered accumulation of intermediates in the digestion of amylopectin in the presence of black tea acting as an effector have been monitored.
Direct observations of starch remodeling should facilitate our understanding and control of processes of biotechnological, medical, and environmental relevance. Here, analytical challenges in time‐resolved in situ observation of starch metabolism are addressed by means of resolution‐optimized multidimensional NMR experiments.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>24166781</pmid><doi>10.1002/cbic.201300461</doi><tpages>6</tpages></addata></record> |
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subjects | amylases Amylopectin - analysis Amylopectin - metabolism Aqueous solutions carbohydrates Humans Magnetic Resonance Spectroscopy - methods NMR NMR spectroscopy Nuclear magnetic resonance pathway intermediates Polysaccharides - analysis Polysaccharides - metabolism Saliva - chemistry Saliva - metabolism Starch - analysis Starch - metabolism |
title | Time-Resolved in-Situ Observation of Starch Polysaccharide Degradation Pathways |
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