Site-Specific Glycan-Peptide Analysis for Determination of N‑Glycoproteome Heterogeneity

A combined glycomics and glycoproteomics strategy was developed for the site-specific analysis of N-linked glycosylation heterogeneity from a complex mammalian protein mixture. Initially, global characterization of the N-glycome was performed using porous graphitized carbon liquid chromatography–tan...

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Veröffentlicht in:	Journal of proteome research 2013-12, Vol.12 (12), p.5791-5800
Hauptverfasser:	Parker, Benjamin L, Thaysen-Andersen, Morten, Solis, Nestor, Scott, Nichollas E, Larsen, Martin R, Graham, Mark E, Packer, Nicolle H, Cordwell, Stuart J
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Brain - metabolism Brain Chemistry Carbohydrate Sequence Chromatography, Liquid - instrumentation Chromatography, Liquid - methods Databases, Factual Genetic Heterogeneity Glycomics - instrumentation Glycomics - methods Glycosylation Humans Hydrogen-Ion Concentration Male Molecular Sequence Annotation Molecular Sequence Data Peptide Mapping - methods Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase - chemistry Protein Processing, Post-Translational Proteome - analysis Proteome - chemistry Rats Rats, Inbred Lew Tandem Mass Spectrometry
Online-Zugang:	Volltext
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