I-DNASE21 system: Development and SWGDAM validation of a new STR 21-plex reaction
Abstract I-DNASE21 is a new STR multiplex system that amplifies 21 STR autosomal loci, plus the amelogenin locus in one reaction. This system has been designed to analyze all the STR loci included in the Combined DNA Index System (CODIS), Interpol Standard Set of Loci (ISSL), Extended European Stand...
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| Veröffentlicht in: | Forensic science international : genetics 2014-01, Vol.8 (1), p.10-19 |
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| container_title | Forensic science international : genetics |
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| creator | Aznar, Jose María Celorrio, David Odriozola, Adrian Köhnemann, Stephan Bravo, María Luisa Builes, Juan Jose Pfeiffer, Heidi Herrera, René J de Pancorbo, Marian M |
| description | Abstract I-DNASE21 is a new STR multiplex system that amplifies 21 STR autosomal loci, plus the amelogenin locus in one reaction. This system has been designed to analyze all the STR loci included in the Combined DNA Index System (CODIS), Interpol Standard Set of Loci (ISSL), Extended European Standard Set (ESS-Extended), UK National Criminal Intelligence DNA Database (NDNAD) and German Core loci (GCL). This manuscript presents the validation of the I-DNASE21 system according to the revised guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM). The results of this validation, added to the extremely high discriminatory power showed, suggest that I-DNASE21 could be a potentially helpful tool for identification and kinship determination even in complex paternity cases. |
| doi_str_mv | 10.1016/j.fsigen.2013.06.014 |
| format | Article |
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This system has been designed to analyze all the STR loci included in the Combined DNA Index System (CODIS), Interpol Standard Set of Loci (ISSL), Extended European Standard Set (ESS-Extended), UK National Criminal Intelligence DNA Database (NDNAD) and German Core loci (GCL). This manuscript presents the validation of the I-DNASE21 system according to the revised guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM). 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The results of this validation, added to the extremely high discriminatory power showed, suggest that I-DNASE21 could be a potentially helpful tool for identification and kinship determination even in complex paternity cases.</description><subject>Animals</subject><subject>Databases, Genetic</subject><subject>Deoxyribonucleases - genetics</subject><subject>Heterozygote</subject><subject>Humans</subject><subject>Microsatellite Repeats</subject><subject>Pathology</subject><subject>Polymerase Chain Reaction</subject><subject>Reproducibility of Results</subject><subject>Species Specificity</subject><issn>1872-4973</issn><issn>1878-0326</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kclOwzAQhi0EYn8DhHzkkuAlXsoBqWrZJBZBQRwt156glCwlTgt9exwK-GJL_mZG8_0IHVGSUkLl6SzNQ_EGdcoI5SmRKaHZBtqlWumEcCY3f94syQaK76C9EGaEiIGiYhvtsIxTITTfRY83yfh-OLlgFIdV6KA6w2NYQtnMK6g7bGuPJ69X4-EdXtqy8LYrmho3Oba4hk88eX7CjCbzEr5wC9b1vwdoK7dlgMPfex-9XF48j66T24erm9HwNnFcsi6x4N2AaZ27aS7EVHkppSZOOC-0ywkXXKkM_GAaj1ci94wLqTmzikmireP76GTdd942HwsInamK4KAsbQ3NIhiaSckVi3hEszXq2iaEFnIzb4vKtitDiellmplZyzS9TEOkiTJj2fHvhMW0Av9f9GcvAudrAOKeywJa48qiLpwt32EFYdYs2joqMNQEZoiZ9Hn0cVBOYn-t-TddT4Sn</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Aznar, Jose María</creator><creator>Celorrio, David</creator><creator>Odriozola, Adrian</creator><creator>Köhnemann, Stephan</creator><creator>Bravo, María Luisa</creator><creator>Builes, Juan Jose</creator><creator>Pfeiffer, Heidi</creator><creator>Herrera, René J</creator><creator>de Pancorbo, Marian M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140101</creationdate><title>I-DNASE21 system: Development and SWGDAM validation of a new STR 21-plex reaction</title><author>Aznar, Jose María ; Celorrio, David ; Odriozola, Adrian ; Köhnemann, Stephan ; Bravo, María Luisa ; Builes, Juan Jose ; Pfeiffer, Heidi ; Herrera, René J ; de Pancorbo, Marian M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-aedc9288fcbf55b7d66680c5cd58cf0353774ed9bbbbd75fd2356832a72608ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Databases, Genetic</topic><topic>Deoxyribonucleases - genetics</topic><topic>Heterozygote</topic><topic>Humans</topic><topic>Microsatellite Repeats</topic><topic>Pathology</topic><topic>Polymerase Chain Reaction</topic><topic>Reproducibility of Results</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aznar, Jose María</creatorcontrib><creatorcontrib>Celorrio, David</creatorcontrib><creatorcontrib>Odriozola, Adrian</creatorcontrib><creatorcontrib>Köhnemann, Stephan</creatorcontrib><creatorcontrib>Bravo, María Luisa</creatorcontrib><creatorcontrib>Builes, Juan Jose</creatorcontrib><creatorcontrib>Pfeiffer, Heidi</creatorcontrib><creatorcontrib>Herrera, René J</creatorcontrib><creatorcontrib>de Pancorbo, Marian M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Forensic science international : genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aznar, Jose María</au><au>Celorrio, David</au><au>Odriozola, Adrian</au><au>Köhnemann, Stephan</au><au>Bravo, María Luisa</au><au>Builes, Juan Jose</au><au>Pfeiffer, Heidi</au><au>Herrera, René J</au><au>de Pancorbo, Marian M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>I-DNASE21 system: Development and SWGDAM validation of a new STR 21-plex reaction</atitle><jtitle>Forensic science international : genetics</jtitle><addtitle>Forensic Sci Int Genet</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>8</volume><issue>1</issue><spage>10</spage><epage>19</epage><pages>10-19</pages><issn>1872-4973</issn><eissn>1878-0326</eissn><abstract>Abstract I-DNASE21 is a new STR multiplex system that amplifies 21 STR autosomal loci, plus the amelogenin locus in one reaction. 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| issn | 1872-4973 1878-0326 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Databases, Genetic Deoxyribonucleases - genetics Heterozygote Humans Microsatellite Repeats Pathology Polymerase Chain Reaction Reproducibility of Results Species Specificity |
| title | I-DNASE21 system: Development and SWGDAM validation of a new STR 21-plex reaction |
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