physical map of the sorghum chloroplast genome

The chloroplast genome of the IS1112C cytoplasm of sorghum was mapped by the construction of a Bam-HI library in pUC8, and hybridization with BamHI, SalI, and PstI digests of chloroplast DNA (ctDNA) of sorghum and maize. The molecules are extensively colinear, with only one of 13 SalI fragments diff...

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Veröffentlicht in:Plant molecular biology 1986-01, Vol.6 (2), p.119-123
Hauptverfasser: Dang, L.H, Pring, D.R
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description The chloroplast genome of the IS1112C cytoplasm of sorghum was mapped by the construction of a Bam-HI library in pUC8, and hybridization with BamHI, SalI, and PstI digests of chloroplast DNA (ctDNA) of sorghum and maize. The molecules are extensively colinear, with only one of 13 SalI fragments differing slightly from maize. Seven of 70 restriction sites differed in the two species. A total molecular size of ca. 138 kb was estimated for sorghum. The inverted repeat was not conserved between sorghum and maize, as revealed by a slightly larger BamHI 16S rDNA fragment in sorghum. Homology of a sequence adjacent to the γbcl gene and one end of the inverted repeat was detected. These homologies were also observed in maize, and suggest that the ctDNA genomes of sorghum and maize share small reiterations of sequences of the inverted repeat.
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subjects Agronomy. Soil science and plant productions
Biological and medical sciences
chloroplast DNA
chromosome mapping
Classical and quantitative genetics. Population genetics. Molecular genetics
Fundamental and applied biological sciences. Psychology
Generalities. Genetics. Plant material
Genes. Genome
Genetics and breeding of economic plants
genome
genomics
Molecular and cellular biology
Molecular genetics
Sorghum bicolor
title physical map of the sorghum chloroplast genome
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