physical map of the sorghum chloroplast genome
The chloroplast genome of the IS1112C cytoplasm of sorghum was mapped by the construction of a Bam-HI library in pUC8, and hybridization with BamHI, SalI, and PstI digests of chloroplast DNA (ctDNA) of sorghum and maize. The molecules are extensively colinear, with only one of 13 SalI fragments diff...
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Veröffentlicht in: | Plant molecular biology 1986-01, Vol.6 (2), p.119-123 |
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description | The chloroplast genome of the IS1112C cytoplasm of sorghum was mapped by the construction of a Bam-HI library in pUC8, and hybridization with BamHI, SalI, and PstI digests of chloroplast DNA (ctDNA) of sorghum and maize. The molecules are extensively colinear, with only one of 13 SalI fragments differing slightly from maize. Seven of 70 restriction sites differed in the two species. A total molecular size of ca. 138 kb was estimated for sorghum. The inverted repeat was not conserved between sorghum and maize, as revealed by a slightly larger BamHI 16S rDNA fragment in sorghum. Homology of a sequence adjacent to the γbcl gene and one end of the inverted repeat was detected. These homologies were also observed in maize, and suggest that the ctDNA genomes of sorghum and maize share small reiterations of sequences of the inverted repeat. |
doi_str_mv | 10.1007/BF00027305 |
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The molecules are extensively colinear, with only one of 13 SalI fragments differing slightly from maize. Seven of 70 restriction sites differed in the two species. A total molecular size of ca. 138 kb was estimated for sorghum. The inverted repeat was not conserved between sorghum and maize, as revealed by a slightly larger BamHI 16S rDNA fragment in sorghum. Homology of a sequence adjacent to the γbcl gene and one end of the inverted repeat was detected. These homologies were also observed in maize, and suggest that the ctDNA genomes of sorghum and maize share small reiterations of sequences of the inverted repeat.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/BF00027305</identifier><identifier>PMID: 24307228</identifier><identifier>CODEN: PMBIDB</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Agronomy. 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The molecules are extensively colinear, with only one of 13 SalI fragments differing slightly from maize. Seven of 70 restriction sites differed in the two species. A total molecular size of ca. 138 kb was estimated for sorghum. The inverted repeat was not conserved between sorghum and maize, as revealed by a slightly larger BamHI 16S rDNA fragment in sorghum. Homology of a sequence adjacent to the γbcl gene and one end of the inverted repeat was detected. These homologies were also observed in maize, and suggest that the ctDNA genomes of sorghum and maize share small reiterations of sequences of the inverted repeat.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>chloroplast DNA</subject><subject>chromosome mapping</subject><subject>Classical and quantitative genetics. Population genetics. Molecular genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities. Genetics. Plant material</subject><subject>Genes. 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Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>chloroplast DNA</topic><topic>chromosome mapping</topic><topic>Classical and quantitative genetics. Population genetics. Molecular genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Generalities. Genetics. Plant material</topic><topic>Genes. Genome</topic><topic>Genetics and breeding of economic plants</topic><topic>genome</topic><topic>genomics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Sorghum bicolor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dang, L.H</creatorcontrib><creatorcontrib>Pring, D.R</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dang, L.H</au><au>Pring, D.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>physical map of the sorghum chloroplast genome</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1986-01-01</date><risdate>1986</risdate><volume>6</volume><issue>2</issue><spage>119</spage><epage>123</epage><pages>119-123</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><coden>PMBIDB</coden><abstract>The chloroplast genome of the IS1112C cytoplasm of sorghum was mapped by the construction of a Bam-HI library in pUC8, and hybridization with BamHI, SalI, and PstI digests of chloroplast DNA (ctDNA) of sorghum and maize. The molecules are extensively colinear, with only one of 13 SalI fragments differing slightly from maize. Seven of 70 restriction sites differed in the two species. A total molecular size of ca. 138 kb was estimated for sorghum. The inverted repeat was not conserved between sorghum and maize, as revealed by a slightly larger BamHI 16S rDNA fragment in sorghum. Homology of a sequence adjacent to the γbcl gene and one end of the inverted repeat was detected. These homologies were also observed in maize, and suggest that the ctDNA genomes of sorghum and maize share small reiterations of sequences of the inverted repeat.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>24307228</pmid><doi>10.1007/BF00027305</doi><tpages>5</tpages></addata></record> |
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subjects | Agronomy. Soil science and plant productions Biological and medical sciences chloroplast DNA chromosome mapping Classical and quantitative genetics. Population genetics. Molecular genetics Fundamental and applied biological sciences. Psychology Generalities. Genetics. Plant material Genes. Genome Genetics and breeding of economic plants genome genomics Molecular and cellular biology Molecular genetics Sorghum bicolor |
title | physical map of the sorghum chloroplast genome |
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