Chemically stimulated feeding behavior in marine animals. Importance of chemical mixtures and involvement of mixture interactions

A review is provided of the chemical components in tissue extracts that elicit feeding behavior in marine fish and crustaceans. For most species, the major stimulants of feeding behavior in excitatory extracts are an assemblage of common metabolites of low molecular weight including amino acids, quaternary ammonium compounds, nucleosides and nucleotides, and organic acids. It is often mixtures of substances rather than individual components that account for the stimulatory capacity of a natural extract. Recent studies using a shrimp,Palaemonetes pugio, are described in which behavioral bioassays were conducted with complex synthetic mixtures formulated on the basis of the composition of four tissue extracts. These results indicate that synergistic interactions occur among the mixture components. The neural mechanisms whereby marine crustaceans receive and code information about chemical mixtures are also reviewed. Narrowly tuned receptor cells, excited only by particular components of food extracts such as specific amino acids, nucleotides, quaternary ammonium compounds, and ammonium ions, are common in lobsters and could transmit information about mixtures as a labeled-line code. However, since physiological recordings indicate that most higher-level neurons in the brain each transmit information about many components of mixtures, rather than about a single component, it is suggested that information about a complex food odor is transmitted as an across-fiber pattern, instead of a labeled-line code. Electrophysiological recordings of responses of peripheral and central neurons of lobsters to odor mixtures and their components reveal that suppressive interactions occur, rather than the synergistic interactions noted earlier in the behavioral studies. Possible reasons for these differences are discussed. Evidence from the behavioral study indicates that the "direction" of a mixture interaction can be concentration-dependent and the synergism may occur at low mixture concentrations, while suppression may occur at high concentrations.