Effects of Salts from the Hofmeister Series on the Conformational Stability, Aggregation Propensity, and Local Flexibility of an IgG1 Monoclonal Antibody

This work examines the effect of three anions from the Hofmeister series (sulfate, chloride, and thiocyanate) on the conformational stability and aggregation rate of an IgG1 monoclonal antibody (mAb) and corresponding changes in the mAb’s backbone flexibility (at pH 6 and 25 °C). Compared to a 0.1 M...

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Veröffentlicht in:	Biochemistry (Easton) 2013-05, Vol.52 (19), p.3376-3389
Hauptverfasser:	Majumdar, Ranajoy, Manikwar, Prakash, Hickey, John M, Samra, Hardeep S, Sathish, Hasige A, Bishop, Steven M, Middaugh, C. Russell, Volkin, David B, Weis, David D
Format:	Artikel
Sprache:	eng
Schlagworte:	Anions Antibodies, Monoclonal - chemistry Humans Immunoglobulin G - chemistry Models, Molecular Protein Conformation Protein Multimerization Protein Stability Protein Structure, Tertiary Salts Thermodynamics
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