effect of right terminal repeat deletion on the oncogenicity of the T-region of pTiT37
A modified pTiT37 plasmid was constructed by deleting a 103 base fragment between an AhaIII and a Bc/I site. This fragment, located to the right of the nopaline synthase gene contains the right terminal 25 base pair repeat sequence which defines the right limit of the T-Region. The effect of this de...
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| Veröffentlicht in: | Plant molecular biology 1985, Vol.5 (1), p.3-11 |
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| container_title | Plant molecular biology |
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| creator | Hepburn, A.G White, J |
| description | A modified pTiT37 plasmid was constructed by deleting a 103 base fragment between an AhaIII and a Bc/I site. This fragment, located to the right of the nopaline synthase gene contains the right terminal 25 base pair repeat sequence which defines the right limit of the T-Region. The effect of this deletion was determined on a number of host plants. In contrast to previous reports, the deletion does not destroy tumorigenicity on all plant species. It had no effect on tumorigenicity when Linum usitatissimum was used as the test species and an attenuating effect when Kalanchoë tubiflora was used. Only when Nicotiana tabacum was used did the mutant appear avirulent. We propose from these data and the phenotype of those tumours that form, that a pseudo border located in the 3' untranslated region of the ipt locus has been used to provide the right hand limit of the T-Region in the absence of the normal border. |
| doi_str_mv | 10.1007/BF00017868 |
| format | Article |
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This fragment, located to the right of the nopaline synthase gene contains the right terminal 25 base pair repeat sequence which defines the right limit of the T-Region. The effect of this deletion was determined on a number of host plants. In contrast to previous reports, the deletion does not destroy tumorigenicity on all plant species. It had no effect on tumorigenicity when Linum usitatissimum was used as the test species and an attenuating effect when Kalanchoë tubiflora was used. Only when Nicotiana tabacum was used did the mutant appear avirulent. We propose from these data and the phenotype of those tumours that form, that a pseudo border located in the 3' untranslated region of the ipt locus has been used to provide the right hand limit of the T-Region in the absence of the normal border.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/BF00017868</identifier><identifier>PMID: 24306535</identifier><identifier>CODEN: PMBIDB</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Agrobacterium tumefaciens ; Agronomy. Soil science and plant productions ; Bacteriology ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; Bryophyllum tubiflorum ; Fundamental and applied biological sciences. Psychology ; Generalities ; Generalities. Genetics. 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This fragment, located to the right of the nopaline synthase gene contains the right terminal 25 base pair repeat sequence which defines the right limit of the T-Region. The effect of this deletion was determined on a number of host plants. In contrast to previous reports, the deletion does not destroy tumorigenicity on all plant species. It had no effect on tumorigenicity when Linum usitatissimum was used as the test species and an attenuating effect when Kalanchoë tubiflora was used. Only when Nicotiana tabacum was used did the mutant appear avirulent. We propose from these data and the phenotype of those tumours that form, that a pseudo border located in the 3' untranslated region of the ipt locus has been used to provide the right hand limit of the T-Region in the absence of the normal border.</description><subject>Agrobacterium tumefaciens</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>Bryophyllum tubiflorum</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities</subject><subject>Generalities. Genetics. Plant material</subject><subject>Genetics</subject><subject>Genetics and breeding of economic plants</subject><subject>Linum usitatissimum</subject><subject>Microbiology</subject><subject>Mission oriented research</subject><subject>neoplasms</subject><subject>Nicotiana tabacum</subject><subject>Plant material</subject><subject>plasmids</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><recordid>eNqF0E1r3DAQBmBRWppN2kt_QONDKSHgdKSxJfnYhnxBIIdsejWSPdqoeO2tpD3k31fubpJjYGBgeDSMXsa-cDjjAOrHr0sA4EpL_Y4teK2wrEHo92wBXKqyqrg4YIcx_skIAOVHdiAqBFljvWC_yTnqUjG5IvjVYyoShbUfzVAE2pBJRU8DJT-NRa70SLl104pG3_n0NL-aZ8sy0Oq_ccVm6ZeoPrEPzgyRPu_7EXu4vFieX5e3d1c35z9vyw5lk0qBHIVV0GhshM03cdUbJAsNCY7OGqmNUrZBDQ0I6SRYUBZ6UYHurUA8Yie7vZsw_d1STO3ax46GwYw0bWPLK1lrybWCTL-_QVE1qpnh6Q52YYoxkGs3wa9NeGo5tHPg7WvgGX_db93aNfUv9DnhDL7tgYmdGVwwY-fji9N1jkDOHzneMWem1qxCJg_3AjhCvktXUuA_QluNhA</recordid><startdate>1985</startdate><enddate>1985</enddate><creator>Hepburn, A.G</creator><creator>White, J</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>1985</creationdate><title>effect of right terminal repeat deletion on the oncogenicity of the T-region of pTiT37</title><author>Hepburn, A.G ; White, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c369t-23132b7098392b30617da3eb09e213fba68a77b93809026f60b07b0d2408db233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Agrobacterium tumefaciens</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>Bryophyllum tubiflorum</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Generalities</topic><topic>Generalities. Genetics. Plant material</topic><topic>Genetics</topic><topic>Genetics and breeding of economic plants</topic><topic>Linum usitatissimum</topic><topic>Microbiology</topic><topic>Mission oriented research</topic><topic>neoplasms</topic><topic>Nicotiana tabacum</topic><topic>Plant material</topic><topic>plasmids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hepburn, A.G</creatorcontrib><creatorcontrib>White, J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hepburn, A.G</au><au>White, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>effect of right terminal repeat deletion on the oncogenicity of the T-region of pTiT37</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1985</date><risdate>1985</risdate><volume>5</volume><issue>1</issue><spage>3</spage><epage>11</epage><pages>3-11</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><coden>PMBIDB</coden><abstract>A modified pTiT37 plasmid was constructed by deleting a 103 base fragment between an AhaIII and a Bc/I site. This fragment, located to the right of the nopaline synthase gene contains the right terminal 25 base pair repeat sequence which defines the right limit of the T-Region. The effect of this deletion was determined on a number of host plants. In contrast to previous reports, the deletion does not destroy tumorigenicity on all plant species. It had no effect on tumorigenicity when Linum usitatissimum was used as the test species and an attenuating effect when Kalanchoë tubiflora was used. Only when Nicotiana tabacum was used did the mutant appear avirulent. We propose from these data and the phenotype of those tumours that form, that a pseudo border located in the 3' untranslated region of the ipt locus has been used to provide the right hand limit of the T-Region in the absence of the normal border.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>24306535</pmid><doi>10.1007/BF00017868</doi><tpages>9</tpages></addata></record> |
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| ispartof | Plant molecular biology, 1985, Vol.5 (1), p.3-11 |
| issn | 0167-4412 1573-5028 |
| language | eng |
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| subjects | Agrobacterium tumefaciens Agronomy. Soil science and plant productions Bacteriology Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Bryophyllum tubiflorum Fundamental and applied biological sciences. Psychology Generalities Generalities. Genetics. Plant material Genetics Genetics and breeding of economic plants Linum usitatissimum Microbiology Mission oriented research neoplasms Nicotiana tabacum Plant material plasmids |
| title | effect of right terminal repeat deletion on the oncogenicity of the T-region of pTiT37 |
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