Antioxidant Activity of Selected Stilbenoids and Their Bioproduction in Hairy Root Cultures of Muscadine Grape (Vitis rotundifolia Michx.)
Stilbenoids are polyphenolic phytoalexins with health-related properties in humans. Muscadine grape (Vitis rotundifolia) hairy root cultures were established via Agrobacterium rhizogenes-mediated transformation, and the effects of growth regulators (3-indolebutyric acid and 6-benzylaminopurine) and...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2013-12, Vol.61 (48), p.11744-11758 |
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| creator | Nopo-Olazabal, Cesar Hubstenberger, John Nopo-Olazabal, Luis Medina-Bolivar, Fabricio |
| description | Stilbenoids are polyphenolic phytoalexins with health-related properties in humans. Muscadine grape (Vitis rotundifolia) hairy root cultures were established via Agrobacterium rhizogenes-mediated transformation, and the effects of growth regulators (3-indolebutyric acid and 6-benzylaminopurine) and methyl jasmonate (MeJA) on stilbenoid production were studied. Twenty-one-day-old hairy root cultures were treated with 100 μM MeJA for 24 h, and then the stilbenoids were extracted from the medium and tissue with ethyl acetate and analyzed by HPLC. Resveratrol, piceid, and ε-viniferin were observed preferentially in tissue, whereas piceatannol was observed only in medium. Growth regulators did not affect the yield of stilbenoids, whereas higher levels were found upon treatment with MeJA. Stilbenoids identified in the hairy root cultures were analyzed for their radical scavenging capacity showing piceatannol and ε-viniferin as the strongest antioxidants. Muscadine grape hairy root cultures were demonstrated to be amenable systems to study stilbenoid biosynthesis and a sustainable source of these bioactive compounds. |
| doi_str_mv | 10.1021/jf400760k |
| format | Article |
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Muscadine grape (Vitis rotundifolia) hairy root cultures were established via Agrobacterium rhizogenes-mediated transformation, and the effects of growth regulators (3-indolebutyric acid and 6-benzylaminopurine) and methyl jasmonate (MeJA) on stilbenoid production were studied. Twenty-one-day-old hairy root cultures were treated with 100 μM MeJA for 24 h, and then the stilbenoids were extracted from the medium and tissue with ethyl acetate and analyzed by HPLC. Resveratrol, piceid, and ε-viniferin were observed preferentially in tissue, whereas piceatannol was observed only in medium. Growth regulators did not affect the yield of stilbenoids, whereas higher levels were found upon treatment with MeJA. Stilbenoids identified in the hairy root cultures were analyzed for their radical scavenging capacity showing piceatannol and ε-viniferin as the strongest antioxidants. Muscadine grape hairy root cultures were demonstrated to be amenable systems to study stilbenoid biosynthesis and a sustainable source of these bioactive compounds.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf400760k</identifier><identifier>PMID: 23668830</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Agrobacterium ; antioxidant activity ; antioxidants ; Antioxidants - analysis ; Antioxidants - metabolism ; benzyladenine ; biosynthesis ; Cell Culture Techniques ; Chromatography, High Pressure Liquid ; ethyl acetate ; growth regulators ; high performance liquid chromatography ; humans ; methyl jasmonate ; phytoalexins ; Plant Extracts - analysis ; Plant Extracts - metabolism ; Plant Roots - chemistry ; Plant Roots - growth & development ; Plant Roots - metabolism ; resveratrol ; Stilbenes - analysis ; Stilbenes - metabolism ; viniferins ; Vitis - chemistry ; Vitis - growth & development ; Vitis - metabolism ; Vitis rotundifolia</subject><ispartof>Journal of agricultural and food chemistry, 2013-12, Vol.61 (48), p.11744-11758</ispartof><rights>Copyright © 2013 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a339t-181fc480277b14d6e35bcc713413ba90f011d2922bceb8942d636700d49585d03</citedby><cites>FETCH-LOGICAL-a339t-181fc480277b14d6e35bcc713413ba90f011d2922bceb8942d636700d49585d03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf400760k$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf400760k$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23668830$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nopo-Olazabal, Cesar</creatorcontrib><creatorcontrib>Hubstenberger, John</creatorcontrib><creatorcontrib>Nopo-Olazabal, Luis</creatorcontrib><creatorcontrib>Medina-Bolivar, Fabricio</creatorcontrib><title>Antioxidant Activity of Selected Stilbenoids and Their Bioproduction in Hairy Root Cultures of Muscadine Grape (Vitis rotundifolia Michx.)</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Stilbenoids are polyphenolic phytoalexins with health-related properties in humans. Muscadine grape (Vitis rotundifolia) hairy root cultures were established via Agrobacterium rhizogenes-mediated transformation, and the effects of growth regulators (3-indolebutyric acid and 6-benzylaminopurine) and methyl jasmonate (MeJA) on stilbenoid production were studied. Twenty-one-day-old hairy root cultures were treated with 100 μM MeJA for 24 h, and then the stilbenoids were extracted from the medium and tissue with ethyl acetate and analyzed by HPLC. Resveratrol, piceid, and ε-viniferin were observed preferentially in tissue, whereas piceatannol was observed only in medium. Growth regulators did not affect the yield of stilbenoids, whereas higher levels were found upon treatment with MeJA. Stilbenoids identified in the hairy root cultures were analyzed for their radical scavenging capacity showing piceatannol and ε-viniferin as the strongest antioxidants. Muscadine grape hairy root cultures were demonstrated to be amenable systems to study stilbenoid biosynthesis and a sustainable source of these bioactive compounds.</description><subject>Agrobacterium</subject><subject>antioxidant activity</subject><subject>antioxidants</subject><subject>Antioxidants - analysis</subject><subject>Antioxidants - metabolism</subject><subject>benzyladenine</subject><subject>biosynthesis</subject><subject>Cell Culture Techniques</subject><subject>Chromatography, High Pressure Liquid</subject><subject>ethyl acetate</subject><subject>growth regulators</subject><subject>high performance liquid chromatography</subject><subject>humans</subject><subject>methyl jasmonate</subject><subject>phytoalexins</subject><subject>Plant Extracts - analysis</subject><subject>Plant Extracts - metabolism</subject><subject>Plant Roots - chemistry</subject><subject>Plant Roots - growth & development</subject><subject>Plant Roots - metabolism</subject><subject>resveratrol</subject><subject>Stilbenes - analysis</subject><subject>Stilbenes - metabolism</subject><subject>viniferins</subject><subject>Vitis - chemistry</subject><subject>Vitis - growth & development</subject><subject>Vitis - metabolism</subject><subject>Vitis rotundifolia</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkMFu1DAQQC0EokvhwA-AL0jtIWXGSRznuKygRWqFxLZcI8d26CxZe2s7qPsLfDWptvTEyYd580Z-jL1FOEMQ-HEzVACNhF_P2AJrAUWNqJ6zBczDQtUSj9irlDYAoOoGXrIjUUqpVAkL9mfpM4V7stpnvjSZflPe8zDwtRudyc7ydaaxdz6QTVx7y69vHUX-icIuBjvNG8Fz8vxCU9zz7yFkvprGPEWXHjRXUzLaknf8POqd4yc_KFPiMeTJWxrCSJpfkbm9Pzt9zV4MekzuzeN7zG6-fL5eXRSX386_rpaXhS7LNheocDCVAtE0PVZWurLujWmwrLDsdQsDIFrRCtEb16u2ElaWsgGwVVur2kJ5zE4O3vkDd5NLudtSMm4ctXdhSh1WssZG1qqd0dMDamJIKbqh20Xa6rjvELqH9N1T-pl996id-q2zT-S_1jPw_gAMOnT6Z6TU3awF4CxAqVpRzcSHA6FN6jZhin7u8J9TfwGpR5Pl</recordid><startdate>20131204</startdate><enddate>20131204</enddate><creator>Nopo-Olazabal, Cesar</creator><creator>Hubstenberger, John</creator><creator>Nopo-Olazabal, Luis</creator><creator>Medina-Bolivar, Fabricio</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20131204</creationdate><title>Antioxidant Activity of Selected Stilbenoids and Their Bioproduction in Hairy Root Cultures of Muscadine Grape (Vitis rotundifolia Michx.)</title><author>Nopo-Olazabal, Cesar ; Hubstenberger, John ; Nopo-Olazabal, Luis ; Medina-Bolivar, Fabricio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a339t-181fc480277b14d6e35bcc713413ba90f011d2922bceb8942d636700d49585d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Agrobacterium</topic><topic>antioxidant activity</topic><topic>antioxidants</topic><topic>Antioxidants - analysis</topic><topic>Antioxidants - metabolism</topic><topic>benzyladenine</topic><topic>biosynthesis</topic><topic>Cell Culture Techniques</topic><topic>Chromatography, High Pressure Liquid</topic><topic>ethyl acetate</topic><topic>growth regulators</topic><topic>high performance liquid chromatography</topic><topic>humans</topic><topic>methyl jasmonate</topic><topic>phytoalexins</topic><topic>Plant Extracts - analysis</topic><topic>Plant Extracts - metabolism</topic><topic>Plant Roots - chemistry</topic><topic>Plant Roots - growth & development</topic><topic>Plant Roots - metabolism</topic><topic>resveratrol</topic><topic>Stilbenes - analysis</topic><topic>Stilbenes - metabolism</topic><topic>viniferins</topic><topic>Vitis - chemistry</topic><topic>Vitis - growth & development</topic><topic>Vitis - metabolism</topic><topic>Vitis rotundifolia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nopo-Olazabal, Cesar</creatorcontrib><creatorcontrib>Hubstenberger, John</creatorcontrib><creatorcontrib>Nopo-Olazabal, Luis</creatorcontrib><creatorcontrib>Medina-Bolivar, Fabricio</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nopo-Olazabal, Cesar</au><au>Hubstenberger, John</au><au>Nopo-Olazabal, Luis</au><au>Medina-Bolivar, Fabricio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antioxidant Activity of Selected Stilbenoids and Their Bioproduction in Hairy Root Cultures of Muscadine Grape (Vitis rotundifolia Michx.)</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2013-12-04</date><risdate>2013</risdate><volume>61</volume><issue>48</issue><spage>11744</spage><epage>11758</epage><pages>11744-11758</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>Stilbenoids are polyphenolic phytoalexins with health-related properties in humans. Muscadine grape (Vitis rotundifolia) hairy root cultures were established via Agrobacterium rhizogenes-mediated transformation, and the effects of growth regulators (3-indolebutyric acid and 6-benzylaminopurine) and methyl jasmonate (MeJA) on stilbenoid production were studied. Twenty-one-day-old hairy root cultures were treated with 100 μM MeJA for 24 h, and then the stilbenoids were extracted from the medium and tissue with ethyl acetate and analyzed by HPLC. Resveratrol, piceid, and ε-viniferin were observed preferentially in tissue, whereas piceatannol was observed only in medium. Growth regulators did not affect the yield of stilbenoids, whereas higher levels were found upon treatment with MeJA. Stilbenoids identified in the hairy root cultures were analyzed for their radical scavenging capacity showing piceatannol and ε-viniferin as the strongest antioxidants. Muscadine grape hairy root cultures were demonstrated to be amenable systems to study stilbenoid biosynthesis and a sustainable source of these bioactive compounds.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>23668830</pmid><doi>10.1021/jf400760k</doi><tpages>15</tpages></addata></record> |
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| subjects | Agrobacterium antioxidant activity antioxidants Antioxidants - analysis Antioxidants - metabolism benzyladenine biosynthesis Cell Culture Techniques Chromatography, High Pressure Liquid ethyl acetate growth regulators high performance liquid chromatography humans methyl jasmonate phytoalexins Plant Extracts - analysis Plant Extracts - metabolism Plant Roots - chemistry Plant Roots - growth & development Plant Roots - metabolism resveratrol Stilbenes - analysis Stilbenes - metabolism viniferins Vitis - chemistry Vitis - growth & development Vitis - metabolism Vitis rotundifolia |
| title | Antioxidant Activity of Selected Stilbenoids and Their Bioproduction in Hairy Root Cultures of Muscadine Grape (Vitis rotundifolia Michx.) |
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