Suppression of cyclin D1 by plasmid-based short hairpin RNA ameliorated experimental pulmonary vascular remodeling
Our previous study has demonstrated that a plasmid-based short hairpin RNA (shRNA) against cyclin D1 could attenuate the pulmonary artery smooth muscle cell (PASMC) proliferation and pulmonary vascular remodeling in smoking rats. In this report, we examined the efficiency of this shRNA plasmid in mo...
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| Veröffentlicht in: | Microvascular research 2013-11, Vol.90, p.144-149 |
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| creator | Zeng, Da-xiong Xu, Guo-peng Lei, Wei Wang, Ran Wang, Chang-guo Huang, Jian-an |
| description | Our previous study has demonstrated that a plasmid-based short hairpin RNA (shRNA) against cyclin D1 could attenuate the pulmonary artery smooth muscle cell (PASMC) proliferation and pulmonary vascular remodeling in smoking rats. In this report, we examined the efficiency of this shRNA plasmid in monocrotaline-induced pulmonary vascular remodeling. A single injection of monocrotaline induced pulmonary vascular remodeling and cyclin D1 over-expression in pulmonary vascular smooth muscle. The shRNA successfully suppressed the up-regulation of cyclin D1 in pulmonary vessels of monocrotaline-treated rats. Moreover, this shRNA decreased the percentage of muscularized vessels and the wall thickness of pulmonary vessels. So, we concluded that plasmid-based shRNA against cyclin D1 ameliorated pulmonary vascular remodeling in monocrotaline-treated rats. Cyclin D1 might be a potential target for the therapy of pulmonary vascular remodeling and pulmonary hypertension.
•We constructed a plasmid vector for rat cyclin D1 shRNA expression.•Intratracheal injection of shRNA/EXGEN500 successfully infected pulmonary vessels.•Monocrotaline induced cyclin D1 up-regulation in pulmonary vessels.•Cyclin D1-shRNA ameliorated pulmonary vascular remodeling. |
| doi_str_mv | 10.1016/j.mvr.2013.07.012 |
| format | Article |
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•We constructed a plasmid vector for rat cyclin D1 shRNA expression.•Intratracheal injection of shRNA/EXGEN500 successfully infected pulmonary vessels.•Monocrotaline induced cyclin D1 up-regulation in pulmonary vessels.•Cyclin D1-shRNA ameliorated pulmonary vascular remodeling.</description><identifier>ISSN: 0026-2862</identifier><identifier>EISSN: 1095-9319</identifier><identifier>DOI: 10.1016/j.mvr.2013.07.012</identifier><identifier>PMID: 23948595</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Proliferation ; Cyclin D1 - genetics ; Cyclin D1 - metabolism ; Disease Models, Animal ; Down-Regulation ; Genetic Therapy - methods ; Genetic Vectors ; Hypertension, Pulmonary - chemically induced ; Hypertension, Pulmonary - genetics ; Hypertension, Pulmonary - metabolism ; Hypertension, Pulmonary - pathology ; Hypertension, Pulmonary - therapy ; Male ; Monocrotaline ; Muscle, Smooth, Vascular - metabolism ; Muscle, Smooth, Vascular - pathology ; Pulmonary Artery - metabolism ; Pulmonary Artery - pathology ; Rats ; Rats, Sprague-Dawley ; RNA Interference ; RNA, Small Interfering - genetics ; RNA, Small Interfering - metabolism ; Transfection</subject><ispartof>Microvascular research, 2013-11, Vol.90, p.144-149</ispartof><rights>2013 Elsevier Inc.</rights><rights>2013.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-d4a5459844d372c47c1d57895c0a0e1e90f157236ef9142340c918d73ed4fc4d3</citedby><cites>FETCH-LOGICAL-c353t-d4a5459844d372c47c1d57895c0a0e1e90f157236ef9142340c918d73ed4fc4d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mvr.2013.07.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23948595$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zeng, Da-xiong</creatorcontrib><creatorcontrib>Xu, Guo-peng</creatorcontrib><creatorcontrib>Lei, Wei</creatorcontrib><creatorcontrib>Wang, Ran</creatorcontrib><creatorcontrib>Wang, Chang-guo</creatorcontrib><creatorcontrib>Huang, Jian-an</creatorcontrib><title>Suppression of cyclin D1 by plasmid-based short hairpin RNA ameliorated experimental pulmonary vascular remodeling</title><title>Microvascular research</title><addtitle>Microvasc Res</addtitle><description>Our previous study has demonstrated that a plasmid-based short hairpin RNA (shRNA) against cyclin D1 could attenuate the pulmonary artery smooth muscle cell (PASMC) proliferation and pulmonary vascular remodeling in smoking rats. In this report, we examined the efficiency of this shRNA plasmid in monocrotaline-induced pulmonary vascular remodeling. A single injection of monocrotaline induced pulmonary vascular remodeling and cyclin D1 over-expression in pulmonary vascular smooth muscle. The shRNA successfully suppressed the up-regulation of cyclin D1 in pulmonary vessels of monocrotaline-treated rats. Moreover, this shRNA decreased the percentage of muscularized vessels and the wall thickness of pulmonary vessels. So, we concluded that plasmid-based shRNA against cyclin D1 ameliorated pulmonary vascular remodeling in monocrotaline-treated rats. Cyclin D1 might be a potential target for the therapy of pulmonary vascular remodeling and pulmonary hypertension.
•We constructed a plasmid vector for rat cyclin D1 shRNA expression.•Intratracheal injection of shRNA/EXGEN500 successfully infected pulmonary vessels.•Monocrotaline induced cyclin D1 up-regulation in pulmonary vessels.•Cyclin D1-shRNA ameliorated pulmonary vascular remodeling.</description><subject>Animals</subject><subject>Cell Proliferation</subject><subject>Cyclin D1 - genetics</subject><subject>Cyclin D1 - metabolism</subject><subject>Disease Models, Animal</subject><subject>Down-Regulation</subject><subject>Genetic Therapy - methods</subject><subject>Genetic Vectors</subject><subject>Hypertension, Pulmonary - chemically induced</subject><subject>Hypertension, Pulmonary - genetics</subject><subject>Hypertension, Pulmonary - metabolism</subject><subject>Hypertension, Pulmonary - pathology</subject><subject>Hypertension, Pulmonary - therapy</subject><subject>Male</subject><subject>Monocrotaline</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Muscle, Smooth, Vascular - pathology</subject><subject>Pulmonary Artery - metabolism</subject><subject>Pulmonary Artery - pathology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA Interference</subject><subject>RNA, Small Interfering - genetics</subject><subject>RNA, Small Interfering - metabolism</subject><subject>Transfection</subject><issn>0026-2862</issn><issn>1095-9319</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMuO1DAQRS0EYpqBD2CDvGST4PIjicVqNMNLGoHEY2257QrjlhMHO2nRf49HPbBkVYs696rqEPISWAsMujeHdjrmljMQLetbBvwR2QHTqtEC9GOyY4x3DR86fkGelXJgDEBp_pRccKHloLTakfxtW5aMpYQ00zRSd3IxzPQG6P5El2jLFHyztwU9LXcpr_TOhrxU4uvnK2onjCFlu9Yt_l4whwnn1Ua6bHFKs80nerTFbdFmmnFKvuLzz-fkyWhjwRcP85L8eP_u-_XH5vbLh0_XV7eNE0qsjZdWSaUHKb3ouZO9A6_6QSvHLENAzUZQPRcdjhokF5I5DYPvBXo5uhq6JK_PvUtOvzYsq5lCcRijnTFtxYDs5KC7YRAVhTPqciol42iW-ku93wAz96rNwVTV5l61Yb2pqmvm1UP9tp_Q_0v8dVuBt2cA65PHgNkUF3B26ENGtxqfwn_q_wAq8JA_</recordid><startdate>201311</startdate><enddate>201311</enddate><creator>Zeng, Da-xiong</creator><creator>Xu, Guo-peng</creator><creator>Lei, Wei</creator><creator>Wang, Ran</creator><creator>Wang, Chang-guo</creator><creator>Huang, Jian-an</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201311</creationdate><title>Suppression of cyclin D1 by plasmid-based short hairpin RNA ameliorated experimental pulmonary vascular remodeling</title><author>Zeng, Da-xiong ; Xu, Guo-peng ; Lei, Wei ; Wang, Ran ; Wang, Chang-guo ; Huang, Jian-an</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-d4a5459844d372c47c1d57895c0a0e1e90f157236ef9142340c918d73ed4fc4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Cell Proliferation</topic><topic>Cyclin D1 - genetics</topic><topic>Cyclin D1 - metabolism</topic><topic>Disease Models, Animal</topic><topic>Down-Regulation</topic><topic>Genetic Therapy - methods</topic><topic>Genetic Vectors</topic><topic>Hypertension, Pulmonary - chemically induced</topic><topic>Hypertension, Pulmonary - genetics</topic><topic>Hypertension, Pulmonary - metabolism</topic><topic>Hypertension, Pulmonary - pathology</topic><topic>Hypertension, Pulmonary - therapy</topic><topic>Male</topic><topic>Monocrotaline</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Muscle, Smooth, Vascular - pathology</topic><topic>Pulmonary Artery - metabolism</topic><topic>Pulmonary Artery - pathology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA Interference</topic><topic>RNA, Small Interfering - genetics</topic><topic>RNA, Small Interfering - metabolism</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zeng, Da-xiong</creatorcontrib><creatorcontrib>Xu, Guo-peng</creatorcontrib><creatorcontrib>Lei, Wei</creatorcontrib><creatorcontrib>Wang, Ran</creatorcontrib><creatorcontrib>Wang, Chang-guo</creatorcontrib><creatorcontrib>Huang, Jian-an</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microvascular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zeng, Da-xiong</au><au>Xu, Guo-peng</au><au>Lei, Wei</au><au>Wang, Ran</au><au>Wang, Chang-guo</au><au>Huang, Jian-an</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Suppression of cyclin D1 by plasmid-based short hairpin RNA ameliorated experimental pulmonary vascular remodeling</atitle><jtitle>Microvascular research</jtitle><addtitle>Microvasc Res</addtitle><date>2013-11</date><risdate>2013</risdate><volume>90</volume><spage>144</spage><epage>149</epage><pages>144-149</pages><issn>0026-2862</issn><eissn>1095-9319</eissn><abstract>Our previous study has demonstrated that a plasmid-based short hairpin RNA (shRNA) against cyclin D1 could attenuate the pulmonary artery smooth muscle cell (PASMC) proliferation and pulmonary vascular remodeling in smoking rats. In this report, we examined the efficiency of this shRNA plasmid in monocrotaline-induced pulmonary vascular remodeling. A single injection of monocrotaline induced pulmonary vascular remodeling and cyclin D1 over-expression in pulmonary vascular smooth muscle. The shRNA successfully suppressed the up-regulation of cyclin D1 in pulmonary vessels of monocrotaline-treated rats. Moreover, this shRNA decreased the percentage of muscularized vessels and the wall thickness of pulmonary vessels. So, we concluded that plasmid-based shRNA against cyclin D1 ameliorated pulmonary vascular remodeling in monocrotaline-treated rats. Cyclin D1 might be a potential target for the therapy of pulmonary vascular remodeling and pulmonary hypertension.
•We constructed a plasmid vector for rat cyclin D1 shRNA expression.•Intratracheal injection of shRNA/EXGEN500 successfully infected pulmonary vessels.•Monocrotaline induced cyclin D1 up-regulation in pulmonary vessels.•Cyclin D1-shRNA ameliorated pulmonary vascular remodeling.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>23948595</pmid><doi>10.1016/j.mvr.2013.07.012</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Cell Proliferation Cyclin D1 - genetics Cyclin D1 - metabolism Disease Models, Animal Down-Regulation Genetic Therapy - methods Genetic Vectors Hypertension, Pulmonary - chemically induced Hypertension, Pulmonary - genetics Hypertension, Pulmonary - metabolism Hypertension, Pulmonary - pathology Hypertension, Pulmonary - therapy Male Monocrotaline Muscle, Smooth, Vascular - metabolism Muscle, Smooth, Vascular - pathology Pulmonary Artery - metabolism Pulmonary Artery - pathology Rats Rats, Sprague-Dawley RNA Interference RNA, Small Interfering - genetics RNA, Small Interfering - metabolism Transfection |
| title | Suppression of cyclin D1 by plasmid-based short hairpin RNA ameliorated experimental pulmonary vascular remodeling |
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