Eicosapentaenoic acid modifies cytokine activity and inhibits cell proliferation in an oesophageal cancer cell line

The present study investigated the effect of eicosapentaenoic acid (EPA) on nuclear factor-kappa B (NF-κB) activation, inflammatory interleukin-6 (IL-6) production, and cell proliferation in a human oesophageal carcinoma cell line (TE-1). Lipopolysaccharide (LPS)-induced IL-6 production in TE-1 cell...

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Veröffentlicht in:	Anticancer research 2013-10, Vol.33 (10), p.4319-4324
Hauptverfasser:	Kubota, Hisako, Matsumoto, Hideo, Higashida, Masaharu, Murakami, Haruaki, Nakashima, Hiroshi, Oka, Yasuo, Okumura, Hideo, Yamamura, Masahiro, Nakamura, Masahumi, Hirai, Toshihiro
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Schlagworte:	Anti-Inflammatory Agents - pharmacology Antineoplastic Agents - pharmacology Apoptosis Apoptosis - drug effects Apoptosis - immunology Cell Line, Tumor Cell Nucleus - metabolism Cell Proliferation - drug effects Cell Shape - drug effects Eicosapentaenoic Acid - pharmacology Esophageal Neoplasms Humans Interleukin-6 - metabolism Lipopolysaccharides - pharmacology NF-kappa B - metabolism Protein Transport
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container_title	Anticancer research
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creator	Kubota, Hisako Matsumoto, Hideo Higashida, Masaharu Murakami, Haruaki Nakashima, Hiroshi Oka, Yasuo Okumura, Hideo Yamamura, Masahiro Nakamura, Masahumi Hirai, Toshihiro
description	The present study investigated the effect of eicosapentaenoic acid (EPA) on nuclear factor-kappa B (NF-κB) activation, inflammatory interleukin-6 (IL-6) production, and cell proliferation in a human oesophageal carcinoma cell line (TE-1). Lipopolysaccharide (LPS)-induced IL-6 production in TE-1 cells in the presence or absence of EPA was determined using enzyme-linked immunosorbent assay (ELISA). The proliferation of TE-1 cells was determined by the WST-1 assay. TE-1 cells were stained with Hoechst 33342 and propidium iodide to observe apoptosis. Immunohistochemical staining of NF-κB in TE-1 cells was performed. LPS increased IL-6 production in TE-1 cells, and EPA treatment prevented this effect. EPA treatment inhibited NF-κB activation and induced apoptosis of TE-1 cells. EPA inhibits NF-κB activation and IL-6 production in oesophageal cancer cells, their inducing apoptosis. These effects of EPA may be of benefit in improving the outcome of cancer surgery.
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These effects of EPA may be of benefit in improving the outcome of cancer surgery.</description><subject>Anti-Inflammatory Agents - pharmacology</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis - immunology</subject><subject>Cell Line, Tumor</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Shape - drug effects</subject><subject>Eicosapentaenoic Acid - pharmacology</subject><subject>Esophageal Neoplasms</subject><subject>Humans</subject><subject>Interleukin-6 - metabolism</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>NF-kappa B - metabolism</subject><subject>Protein Transport</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtLAzEUhYMotlb_gmTpZiCZyXMppT6g4EbXQyZzY6MzyThJhf57I617Vwfu-Ticc8_QkkpNK8kbco6WpOakkoTwBbpK6YMQIbRqLtGiZrSutVZLlDbexmQmCNlAiN5iY32Px9h75yFhe8jx0wco5-y_fT5gE3rsw853PhcbhgFPcxy8g9lkH0PxCoIjpDjtzDuYAVsTLMxHdihZ1-jCmSHBzUlX6O1h87p-qrYvj8_r-2011YzlygneU-OUqhsCyjppue47TZWjndZUAgHqbFP2EZAchGRGqF5q2_FOdY41K3R3zC0Fv_aQcjv69NvCBIj71FImGKdcEP4PlDWsJkSKgt6e0H03Qt9Osx_NfGj_ftr8AJ5sdWw</recordid><startdate>201310</startdate><enddate>201310</enddate><creator>Kubota, Hisako</creator><creator>Matsumoto, Hideo</creator><creator>Higashida, Masaharu</creator><creator>Murakami, Haruaki</creator><creator>Nakashima, Hiroshi</creator><creator>Oka, Yasuo</creator><creator>Okumura, Hideo</creator><creator>Yamamura, Masahiro</creator><creator>Nakamura, Masahumi</creator><creator>Hirai, Toshihiro</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>201310</creationdate><title>Eicosapentaenoic acid modifies cytokine activity and inhibits cell proliferation in an oesophageal cancer cell line</title><author>Kubota, Hisako ; Matsumoto, Hideo ; Higashida, Masaharu ; Murakami, Haruaki ; Nakashima, Hiroshi ; Oka, Yasuo ; Okumura, Hideo ; Yamamura, Masahiro ; Nakamura, Masahumi ; Hirai, Toshihiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p244t-f65d1af88230e8cf7c59db918f1b9917e0e1fc37000e75e674a68d79cb5b8bf43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Anti-Inflammatory Agents - pharmacology</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Apoptosis - immunology</topic><topic>Cell Line, Tumor</topic><topic>Cell Nucleus - metabolism</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Shape - drug effects</topic><topic>Eicosapentaenoic Acid - pharmacology</topic><topic>Esophageal Neoplasms</topic><topic>Humans</topic><topic>Interleukin-6 - metabolism</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>NF-kappa B - metabolism</topic><topic>Protein Transport</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kubota, Hisako</creatorcontrib><creatorcontrib>Matsumoto, Hideo</creatorcontrib><creatorcontrib>Higashida, Masaharu</creatorcontrib><creatorcontrib>Murakami, Haruaki</creatorcontrib><creatorcontrib>Nakashima, Hiroshi</creatorcontrib><creatorcontrib>Oka, Yasuo</creatorcontrib><creatorcontrib>Okumura, Hideo</creatorcontrib><creatorcontrib>Yamamura, Masahiro</creatorcontrib><creatorcontrib>Nakamura, Masahumi</creatorcontrib><creatorcontrib>Hirai, Toshihiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kubota, Hisako</au><au>Matsumoto, Hideo</au><au>Higashida, Masaharu</au><au>Murakami, Haruaki</au><au>Nakashima, Hiroshi</au><au>Oka, Yasuo</au><au>Okumura, Hideo</au><au>Yamamura, Masahiro</au><au>Nakamura, Masahumi</au><au>Hirai, Toshihiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Eicosapentaenoic acid modifies cytokine activity and inhibits cell proliferation in an oesophageal cancer cell line</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2013-10</date><risdate>2013</risdate><volume>33</volume><issue>10</issue><spage>4319</spage><epage>4324</epage><pages>4319-4324</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><abstract>The present study investigated the effect of eicosapentaenoic acid (EPA) on nuclear factor-kappa B (NF-κB) activation, inflammatory interleukin-6 (IL-6) production, and cell proliferation in a human oesophageal carcinoma cell line (TE-1). Lipopolysaccharide (LPS)-induced IL-6 production in TE-1 cells in the presence or absence of EPA was determined using enzyme-linked immunosorbent assay (ELISA). The proliferation of TE-1 cells was determined by the WST-1 assay. TE-1 cells were stained with Hoechst 33342 and propidium iodide to observe apoptosis. Immunohistochemical staining of NF-κB in TE-1 cells was performed. LPS increased IL-6 production in TE-1 cells, and EPA treatment prevented this effect. EPA treatment inhibited NF-κB activation and induced apoptosis of TE-1 cells. EPA inhibits NF-κB activation and IL-6 production in oesophageal cancer cells, their inducing apoptosis. These effects of EPA may be of benefit in improving the outcome of cancer surgery.</abstract><cop>Greece</cop><pmid>24122998</pmid><tpages>6</tpages></addata></record>
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subjects	Anti-Inflammatory Agents - pharmacology Antineoplastic Agents - pharmacology Apoptosis Apoptosis - drug effects Apoptosis - immunology Cell Line, Tumor Cell Nucleus - metabolism Cell Proliferation - drug effects Cell Shape - drug effects Eicosapentaenoic Acid - pharmacology Esophageal Neoplasms Humans Interleukin-6 - metabolism Lipopolysaccharides - pharmacology NF-kappa B - metabolism Protein Transport
title	Eicosapentaenoic acid modifies cytokine activity and inhibits cell proliferation in an oesophageal cancer cell line
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