Transcriptional Profiling of Rice Early Response to Magnaporthe oryzae Identified OsWRKYs as Important Regulators in Rice Blast Resistance. e59720
Rice blast disease is a major threat to rice production worldwide, but the mechanisms underlying rice resistance to the causal agent Magnaporthe oryzae remain elusive. Therefore, we carried out a transcriptome study on rice early defense response to M. oryzae. We found that the transcriptional profi...
Gespeichert in:
| Veröffentlicht in: | PloS one 2013-03, Vol.8 (3) |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 3 |
| container_start_page | |
| container_title | PloS one |
| container_volume | 8 |
| creator | Wei, Tong Ou, Bin Li, Jinbin Zhao, Yang Guo, Dongshu Zhu, Youyong Chen, Zhangliang Gu, Hongya Li, Chengyun Qin, Genji |
| description | Rice blast disease is a major threat to rice production worldwide, but the mechanisms underlying rice resistance to the causal agent Magnaporthe oryzae remain elusive. Therefore, we carried out a transcriptome study on rice early defense response to M. oryzae. We found that the transcriptional profiles of rice compatible and incompatible interactions with M. oryzae were mostly similar, with genes regulated more prominently in the incompatible interactions. The functional analysis showed that the genes involved in signaling and secondary metabolism were extensively up-regulated. In particular, WRKY transcription factor genes were significantly enriched among the up-regulated genes. Overexpressing one of these WRKY genes, OsWRKY47, in transgenic rice plants conferred enhanced resistance against rice blast fungus. Our results revealed the sophisticated transcriptional reprogramming of signaling and metabolic pathways during rice early response to M. oryzae and demonstrated the critical roles of WRKY transcription factors in rice blast resistance. |
| doi_str_mv | 10.1371/journal.pone.0059720 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_1464514431</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1464514431</sourcerecordid><originalsourceid>FETCH-proquest_miscellaneous_14645144313</originalsourceid><addsrcrecordid>eNqVjs1Kw0AUhQdBsP68gYu7dNM4k0lTu1UqFhElFEpX5ZLexCnTuXHuZFEfwyc2VV_A1Vmc7zscpa6Nzoydmtsd9zGgzzoOlGk9mU1zfaJGZmbzcZlre6bORXZDYe_KcqS-lhGD1NF1yfHgwVvkxnkXWuAGKlcTzDH6A1Qkw6QQJIYXbAN2HNM7AcfDJxIsthSSaxxt4VVW1fNaAAUW-yOFIQ1623tMHAVc-N299yjHQpwMSE0Z0M_bS3XaoBe6-ssLdfM4Xz48jbvIHz1J2uyd1OQ9BuJeNqYoi4kpCmvsP9BvdAdh2w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1464514431</pqid></control><display><type>article</type><title>Transcriptional Profiling of Rice Early Response to Magnaporthe oryzae Identified OsWRKYs as Important Regulators in Rice Blast Resistance. e59720</title><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Wei, Tong ; Ou, Bin ; Li, Jinbin ; Zhao, Yang ; Guo, Dongshu ; Zhu, Youyong ; Chen, Zhangliang ; Gu, Hongya ; Li, Chengyun ; Qin, Genji</creator><creatorcontrib>Wei, Tong ; Ou, Bin ; Li, Jinbin ; Zhao, Yang ; Guo, Dongshu ; Zhu, Youyong ; Chen, Zhangliang ; Gu, Hongya ; Li, Chengyun ; Qin, Genji</creatorcontrib><description>Rice blast disease is a major threat to rice production worldwide, but the mechanisms underlying rice resistance to the causal agent Magnaporthe oryzae remain elusive. Therefore, we carried out a transcriptome study on rice early defense response to M. oryzae. We found that the transcriptional profiles of rice compatible and incompatible interactions with M. oryzae were mostly similar, with genes regulated more prominently in the incompatible interactions. The functional analysis showed that the genes involved in signaling and secondary metabolism were extensively up-regulated. In particular, WRKY transcription factor genes were significantly enriched among the up-regulated genes. Overexpressing one of these WRKY genes, OsWRKY47, in transgenic rice plants conferred enhanced resistance against rice blast fungus. Our results revealed the sophisticated transcriptional reprogramming of signaling and metabolic pathways during rice early response to M. oryzae and demonstrated the critical roles of WRKY transcription factors in rice blast resistance.</description><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0059720</identifier><language>eng</language><subject>Gene expression ; Oryza sativa</subject><ispartof>PloS one, 2013-03, Vol.8 (3)</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids></links><search><creatorcontrib>Wei, Tong</creatorcontrib><creatorcontrib>Ou, Bin</creatorcontrib><creatorcontrib>Li, Jinbin</creatorcontrib><creatorcontrib>Zhao, Yang</creatorcontrib><creatorcontrib>Guo, Dongshu</creatorcontrib><creatorcontrib>Zhu, Youyong</creatorcontrib><creatorcontrib>Chen, Zhangliang</creatorcontrib><creatorcontrib>Gu, Hongya</creatorcontrib><creatorcontrib>Li, Chengyun</creatorcontrib><creatorcontrib>Qin, Genji</creatorcontrib><title>Transcriptional Profiling of Rice Early Response to Magnaporthe oryzae Identified OsWRKYs as Important Regulators in Rice Blast Resistance. e59720</title><title>PloS one</title><description>Rice blast disease is a major threat to rice production worldwide, but the mechanisms underlying rice resistance to the causal agent Magnaporthe oryzae remain elusive. Therefore, we carried out a transcriptome study on rice early defense response to M. oryzae. We found that the transcriptional profiles of rice compatible and incompatible interactions with M. oryzae were mostly similar, with genes regulated more prominently in the incompatible interactions. The functional analysis showed that the genes involved in signaling and secondary metabolism were extensively up-regulated. In particular, WRKY transcription factor genes were significantly enriched among the up-regulated genes. Overexpressing one of these WRKY genes, OsWRKY47, in transgenic rice plants conferred enhanced resistance against rice blast fungus. Our results revealed the sophisticated transcriptional reprogramming of signaling and metabolic pathways during rice early response to M. oryzae and demonstrated the critical roles of WRKY transcription factors in rice blast resistance.</description><subject>Gene expression</subject><subject>Oryza sativa</subject><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqVjs1Kw0AUhQdBsP68gYu7dNM4k0lTu1UqFhElFEpX5ZLexCnTuXHuZFEfwyc2VV_A1Vmc7zscpa6Nzoydmtsd9zGgzzoOlGk9mU1zfaJGZmbzcZlre6bORXZDYe_KcqS-lhGD1NF1yfHgwVvkxnkXWuAGKlcTzDH6A1Qkw6QQJIYXbAN2HNM7AcfDJxIsthSSaxxt4VVW1fNaAAUW-yOFIQ1623tMHAVc-N299yjHQpwMSE0Z0M_bS3XaoBe6-ssLdfM4Xz48jbvIHz1J2uyd1OQ9BuJeNqYoi4kpCmvsP9BvdAdh2w</recordid><startdate>20130301</startdate><enddate>20130301</enddate><creator>Wei, Tong</creator><creator>Ou, Bin</creator><creator>Li, Jinbin</creator><creator>Zhao, Yang</creator><creator>Guo, Dongshu</creator><creator>Zhu, Youyong</creator><creator>Chen, Zhangliang</creator><creator>Gu, Hongya</creator><creator>Li, Chengyun</creator><creator>Qin, Genji</creator><scope>M7N</scope></search><sort><creationdate>20130301</creationdate><title>Transcriptional Profiling of Rice Early Response to Magnaporthe oryzae Identified OsWRKYs as Important Regulators in Rice Blast Resistance. e59720</title><author>Wei, Tong ; Ou, Bin ; Li, Jinbin ; Zhao, Yang ; Guo, Dongshu ; Zhu, Youyong ; Chen, Zhangliang ; Gu, Hongya ; Li, Chengyun ; Qin, Genji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_14645144313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Gene expression</topic><topic>Oryza sativa</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wei, Tong</creatorcontrib><creatorcontrib>Ou, Bin</creatorcontrib><creatorcontrib>Li, Jinbin</creatorcontrib><creatorcontrib>Zhao, Yang</creatorcontrib><creatorcontrib>Guo, Dongshu</creatorcontrib><creatorcontrib>Zhu, Youyong</creatorcontrib><creatorcontrib>Chen, Zhangliang</creatorcontrib><creatorcontrib>Gu, Hongya</creatorcontrib><creatorcontrib>Li, Chengyun</creatorcontrib><creatorcontrib>Qin, Genji</creatorcontrib><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wei, Tong</au><au>Ou, Bin</au><au>Li, Jinbin</au><au>Zhao, Yang</au><au>Guo, Dongshu</au><au>Zhu, Youyong</au><au>Chen, Zhangliang</au><au>Gu, Hongya</au><au>Li, Chengyun</au><au>Qin, Genji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptional Profiling of Rice Early Response to Magnaporthe oryzae Identified OsWRKYs as Important Regulators in Rice Blast Resistance. e59720</atitle><jtitle>PloS one</jtitle><date>2013-03-01</date><risdate>2013</risdate><volume>8</volume><issue>3</issue><eissn>1932-6203</eissn><abstract>Rice blast disease is a major threat to rice production worldwide, but the mechanisms underlying rice resistance to the causal agent Magnaporthe oryzae remain elusive. Therefore, we carried out a transcriptome study on rice early defense response to M. oryzae. We found that the transcriptional profiles of rice compatible and incompatible interactions with M. oryzae were mostly similar, with genes regulated more prominently in the incompatible interactions. The functional analysis showed that the genes involved in signaling and secondary metabolism were extensively up-regulated. In particular, WRKY transcription factor genes were significantly enriched among the up-regulated genes. Overexpressing one of these WRKY genes, OsWRKY47, in transgenic rice plants conferred enhanced resistance against rice blast fungus. Our results revealed the sophisticated transcriptional reprogramming of signaling and metabolic pathways during rice early response to M. oryzae and demonstrated the critical roles of WRKY transcription factors in rice blast resistance.</abstract><doi>10.1371/journal.pone.0059720</doi></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 1932-6203 |
| ispartof | PloS one, 2013-03, Vol.8 (3) |
| issn | 1932-6203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1464514431 |
| source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Gene expression Oryza sativa |
| title | Transcriptional Profiling of Rice Early Response to Magnaporthe oryzae Identified OsWRKYs as Important Regulators in Rice Blast Resistance. e59720 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T16%3A46%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Transcriptional%20Profiling%20of%20Rice%20Early%20Response%20to%20Magnaporthe%20oryzae%20Identified%20OsWRKYs%20as%20Important%20Regulators%20in%20Rice%20Blast%20Resistance.%20e59720&rft.jtitle=PloS%20one&rft.au=Wei,%20Tong&rft.date=2013-03-01&rft.volume=8&rft.issue=3&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0059720&rft_dat=%3Cproquest%3E1464514431%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1464514431&rft_id=info:pmid/&rfr_iscdi=true |