MR Detection of LPS-Induced Neutrophil Activation using Mannan-Coated Superparamagnetic Iron Oxide Nanoparticles
Purpose The purpose of this study was to investigate the potential of the phagocytic activity of lipopolysaccharide (LPS) induced neutrophils that are mannan coated with superparamagnetic iron oxide nanoparticles (M-SPION). Procedures Human neutrophils were divided into control and LPS groups. The n...
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| Veröffentlicht in: | Molecular imaging and biology 2013-12, Vol.15 (6), p.685-692 |
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| creator | Shanhua, Han Huijing, Han Moon, Myeong Ju Heo, Suk Hee Lim, Hyo Soon Park, In-Kyu Cho, Chong-Su Kwak, Sang Hyun Jeong, Yong Yeon |
| description | Purpose
The purpose of this study was to investigate the potential of the phagocytic activity of lipopolysaccharide (LPS) induced neutrophils that are mannan coated with superparamagnetic iron oxide nanoparticles (M-SPION).
Procedures
Human neutrophils were divided into control and LPS groups. The neutrophils were labeled with M-SPION and dextran-coated SPION. After labeling of M-SPION, the mean signal intensity (SI) of the LPS group was significantly lower than that of the control group.
Results
The labeling of both control and LPS groups with M-SPION showed significantly lower SI than those labeled with D-SPION. After labeling with M-SPION, the intracellular iron uptake of neutrophil in Prussian blue staining was markedly demonstrated in the LPS group, but not in the control group. M-SPION was more effective than D-SPION in the labeling of neutrophils
in vitro
.
Conclusions
The
in vitro
labeling technique of LPS neutrophil with M-SPION on MR imaging could be developed into a diagnostic method of LPS-induced neutrophils. |
| doi_str_mv | 10.1007/s11307-013-0643-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1464511027</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3124815651</sourcerecordid><originalsourceid>FETCH-LOGICAL-c405t-beb85f0d3897b1c6ac1fe5973fd27d31bd489cf14732a5d5afdd9c5a3dc4a3f93</originalsourceid><addsrcrecordid>eNp10V1vFCEUBmBiNLZWf4A3ZhJvvEHPgQF2LputH5tsW2P1mjB8rNPMwggzZv33UrcaY-IVhPOcA-El5DnCawRQbwoiB0UBOQXZcnp4QE5xJYEyAPaw7gWXFCVnJ-RJKbcAqJDxx-SEcamAC35KpstPzYWfvZ2HFJsUmu3HG7qJbrHeNVd-mXOavg5jc17Bd_MLLWWIu-bSxGgiXSczV3mzTD5PJpu92UU_D7bZ5EqvD4PzzZWJqdbq6ejLU_IomLH4Z_frGfny7u3n9Qe6vX6_WZ9vqW1BzLT3_UoEcHzVqR6tNBaDF53iwTHlOPauXXU2YKs4M8IJE5zrrDDc2dbw0PEz8uo4d8rp2-LLrPdDsX4cTfRpKRpb2QpEYKrSl__Q27TkWF9XlQCUTApeFR6VzamU7IOe8rA3-YdG0Hdx6GMcusah7-LQh9rz4n7y0u-9-9Px-_8rYEdQainufP7r6v9O_Ql7Qpa6</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1450162653</pqid></control><display><type>article</type><title>MR Detection of LPS-Induced Neutrophil Activation using Mannan-Coated Superparamagnetic Iron Oxide Nanoparticles</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Shanhua, Han ; Huijing, Han ; Moon, Myeong Ju ; Heo, Suk Hee ; Lim, Hyo Soon ; Park, In-Kyu ; Cho, Chong-Su ; Kwak, Sang Hyun ; Jeong, Yong Yeon</creator><creatorcontrib>Shanhua, Han ; Huijing, Han ; Moon, Myeong Ju ; Heo, Suk Hee ; Lim, Hyo Soon ; Park, In-Kyu ; Cho, Chong-Su ; Kwak, Sang Hyun ; Jeong, Yong Yeon</creatorcontrib><description>Purpose
The purpose of this study was to investigate the potential of the phagocytic activity of lipopolysaccharide (LPS) induced neutrophils that are mannan coated with superparamagnetic iron oxide nanoparticles (M-SPION).
Procedures
Human neutrophils were divided into control and LPS groups. The neutrophils were labeled with M-SPION and dextran-coated SPION. After labeling of M-SPION, the mean signal intensity (SI) of the LPS group was significantly lower than that of the control group.
Results
The labeling of both control and LPS groups with M-SPION showed significantly lower SI than those labeled with D-SPION. After labeling with M-SPION, the intracellular iron uptake of neutrophil in Prussian blue staining was markedly demonstrated in the LPS group, but not in the control group. M-SPION was more effective than D-SPION in the labeling of neutrophils
in vitro
.
Conclusions
The
in vitro
labeling technique of LPS neutrophil with M-SPION on MR imaging could be developed into a diagnostic method of LPS-induced neutrophils.</description><identifier>ISSN: 1536-1632</identifier><identifier>EISSN: 1860-2002</identifier><identifier>DOI: 10.1007/s11307-013-0643-x</identifier><identifier>PMID: 23670353</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Cells, Cultured ; Humans ; Imaging ; Intracellular Space - chemistry ; Intracellular Space - metabolism ; Lectins, C-Type - chemistry ; Lectins, C-Type - metabolism ; Lipopolysaccharides - toxicity ; Magnetic Resonance Imaging ; Magnetite Nanoparticles - analysis ; Magnetite Nanoparticles - chemistry ; Mannans - metabolism ; Mannose-Binding Lectins - chemistry ; Mannose-Binding Lectins - metabolism ; Medicine ; Medicine & Public Health ; Neutrophil Activation - drug effects ; Neutrophil Activation - physiology ; Neutrophils - chemistry ; Neutrophils - cytology ; Neutrophils - metabolism ; Radiology ; Receptors, Cell Surface - chemistry ; Receptors, Cell Surface - metabolism ; Research Article</subject><ispartof>Molecular imaging and biology, 2013-12, Vol.15 (6), p.685-692</ispartof><rights>World Molecular Imaging Society 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-beb85f0d3897b1c6ac1fe5973fd27d31bd489cf14732a5d5afdd9c5a3dc4a3f93</citedby><cites>FETCH-LOGICAL-c405t-beb85f0d3897b1c6ac1fe5973fd27d31bd489cf14732a5d5afdd9c5a3dc4a3f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11307-013-0643-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11307-013-0643-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27911,27912,41475,42544,51306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23670353$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shanhua, Han</creatorcontrib><creatorcontrib>Huijing, Han</creatorcontrib><creatorcontrib>Moon, Myeong Ju</creatorcontrib><creatorcontrib>Heo, Suk Hee</creatorcontrib><creatorcontrib>Lim, Hyo Soon</creatorcontrib><creatorcontrib>Park, In-Kyu</creatorcontrib><creatorcontrib>Cho, Chong-Su</creatorcontrib><creatorcontrib>Kwak, Sang Hyun</creatorcontrib><creatorcontrib>Jeong, Yong Yeon</creatorcontrib><title>MR Detection of LPS-Induced Neutrophil Activation using Mannan-Coated Superparamagnetic Iron Oxide Nanoparticles</title><title>Molecular imaging and biology</title><addtitle>Mol Imaging Biol</addtitle><addtitle>Mol Imaging Biol</addtitle><description>Purpose
The purpose of this study was to investigate the potential of the phagocytic activity of lipopolysaccharide (LPS) induced neutrophils that are mannan coated with superparamagnetic iron oxide nanoparticles (M-SPION).
Procedures
Human neutrophils were divided into control and LPS groups. The neutrophils were labeled with M-SPION and dextran-coated SPION. After labeling of M-SPION, the mean signal intensity (SI) of the LPS group was significantly lower than that of the control group.
Results
The labeling of both control and LPS groups with M-SPION showed significantly lower SI than those labeled with D-SPION. After labeling with M-SPION, the intracellular iron uptake of neutrophil in Prussian blue staining was markedly demonstrated in the LPS group, but not in the control group. M-SPION was more effective than D-SPION in the labeling of neutrophils
in vitro
.
Conclusions
The
in vitro
labeling technique of LPS neutrophil with M-SPION on MR imaging could be developed into a diagnostic method of LPS-induced neutrophils.</description><subject>Cells, Cultured</subject><subject>Humans</subject><subject>Imaging</subject><subject>Intracellular Space - chemistry</subject><subject>Intracellular Space - metabolism</subject><subject>Lectins, C-Type - chemistry</subject><subject>Lectins, C-Type - metabolism</subject><subject>Lipopolysaccharides - toxicity</subject><subject>Magnetic Resonance Imaging</subject><subject>Magnetite Nanoparticles - analysis</subject><subject>Magnetite Nanoparticles - chemistry</subject><subject>Mannans - metabolism</subject><subject>Mannose-Binding Lectins - chemistry</subject><subject>Mannose-Binding Lectins - metabolism</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Neutrophil Activation - drug effects</subject><subject>Neutrophil Activation - physiology</subject><subject>Neutrophils - chemistry</subject><subject>Neutrophils - cytology</subject><subject>Neutrophils - metabolism</subject><subject>Radiology</subject><subject>Receptors, Cell Surface - chemistry</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Research Article</subject><issn>1536-1632</issn><issn>1860-2002</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp10V1vFCEUBmBiNLZWf4A3ZhJvvEHPgQF2LputH5tsW2P1mjB8rNPMwggzZv33UrcaY-IVhPOcA-El5DnCawRQbwoiB0UBOQXZcnp4QE5xJYEyAPaw7gWXFCVnJ-RJKbcAqJDxx-SEcamAC35KpstPzYWfvZ2HFJsUmu3HG7qJbrHeNVd-mXOavg5jc17Bd_MLLWWIu-bSxGgiXSczV3mzTD5PJpu92UU_D7bZ5EqvD4PzzZWJqdbq6ejLU_IomLH4Z_frGfny7u3n9Qe6vX6_WZ9vqW1BzLT3_UoEcHzVqR6tNBaDF53iwTHlOPauXXU2YKs4M8IJE5zrrDDc2dbw0PEz8uo4d8rp2-LLrPdDsX4cTfRpKRpb2QpEYKrSl__Q27TkWF9XlQCUTApeFR6VzamU7IOe8rA3-YdG0Hdx6GMcusah7-LQh9rz4n7y0u-9-9Px-_8rYEdQainufP7r6v9O_Ql7Qpa6</recordid><startdate>20131201</startdate><enddate>20131201</enddate><creator>Shanhua, Han</creator><creator>Huijing, Han</creator><creator>Moon, Myeong Ju</creator><creator>Heo, Suk Hee</creator><creator>Lim, Hyo Soon</creator><creator>Park, In-Kyu</creator><creator>Cho, Chong-Su</creator><creator>Kwak, Sang Hyun</creator><creator>Jeong, Yong Yeon</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>L6V</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope></search><sort><creationdate>20131201</creationdate><title>MR Detection of LPS-Induced Neutrophil Activation using Mannan-Coated Superparamagnetic Iron Oxide Nanoparticles</title><author>Shanhua, Han ; Huijing, Han ; Moon, Myeong Ju ; Heo, Suk Hee ; Lim, Hyo Soon ; Park, In-Kyu ; Cho, Chong-Su ; Kwak, Sang Hyun ; Jeong, Yong Yeon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-beb85f0d3897b1c6ac1fe5973fd27d31bd489cf14732a5d5afdd9c5a3dc4a3f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Cells, Cultured</topic><topic>Humans</topic><topic>Imaging</topic><topic>Intracellular Space - chemistry</topic><topic>Intracellular Space - metabolism</topic><topic>Lectins, C-Type - chemistry</topic><topic>Lectins, C-Type - metabolism</topic><topic>Lipopolysaccharides - toxicity</topic><topic>Magnetic Resonance Imaging</topic><topic>Magnetite Nanoparticles - analysis</topic><topic>Magnetite Nanoparticles - chemistry</topic><topic>Mannans - metabolism</topic><topic>Mannose-Binding Lectins - chemistry</topic><topic>Mannose-Binding Lectins - metabolism</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Neutrophil Activation - drug effects</topic><topic>Neutrophil Activation - physiology</topic><topic>Neutrophils - chemistry</topic><topic>Neutrophils - cytology</topic><topic>Neutrophils - metabolism</topic><topic>Radiology</topic><topic>Receptors, Cell Surface - chemistry</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Research Article</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shanhua, Han</creatorcontrib><creatorcontrib>Huijing, Han</creatorcontrib><creatorcontrib>Moon, Myeong Ju</creatorcontrib><creatorcontrib>Heo, Suk Hee</creatorcontrib><creatorcontrib>Lim, Hyo Soon</creatorcontrib><creatorcontrib>Park, In-Kyu</creatorcontrib><creatorcontrib>Cho, Chong-Su</creatorcontrib><creatorcontrib>Kwak, Sang Hyun</creatorcontrib><creatorcontrib>Jeong, Yong Yeon</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><jtitle>Molecular imaging and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shanhua, Han</au><au>Huijing, Han</au><au>Moon, Myeong Ju</au><au>Heo, Suk Hee</au><au>Lim, Hyo Soon</au><au>Park, In-Kyu</au><au>Cho, Chong-Su</au><au>Kwak, Sang Hyun</au><au>Jeong, Yong Yeon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MR Detection of LPS-Induced Neutrophil Activation using Mannan-Coated Superparamagnetic Iron Oxide Nanoparticles</atitle><jtitle>Molecular imaging and biology</jtitle><stitle>Mol Imaging Biol</stitle><addtitle>Mol Imaging Biol</addtitle><date>2013-12-01</date><risdate>2013</risdate><volume>15</volume><issue>6</issue><spage>685</spage><epage>692</epage><pages>685-692</pages><issn>1536-1632</issn><eissn>1860-2002</eissn><abstract>Purpose
The purpose of this study was to investigate the potential of the phagocytic activity of lipopolysaccharide (LPS) induced neutrophils that are mannan coated with superparamagnetic iron oxide nanoparticles (M-SPION).
Procedures
Human neutrophils were divided into control and LPS groups. The neutrophils were labeled with M-SPION and dextran-coated SPION. After labeling of M-SPION, the mean signal intensity (SI) of the LPS group was significantly lower than that of the control group.
Results
The labeling of both control and LPS groups with M-SPION showed significantly lower SI than those labeled with D-SPION. After labeling with M-SPION, the intracellular iron uptake of neutrophil in Prussian blue staining was markedly demonstrated in the LPS group, but not in the control group. M-SPION was more effective than D-SPION in the labeling of neutrophils
in vitro
.
Conclusions
The
in vitro
labeling technique of LPS neutrophil with M-SPION on MR imaging could be developed into a diagnostic method of LPS-induced neutrophils.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>23670353</pmid><doi>10.1007/s11307-013-0643-x</doi><tpages>8</tpages></addata></record> |
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| ispartof | Molecular imaging and biology, 2013-12, Vol.15 (6), p.685-692 |
| issn | 1536-1632 1860-2002 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Cells, Cultured Humans Imaging Intracellular Space - chemistry Intracellular Space - metabolism Lectins, C-Type - chemistry Lectins, C-Type - metabolism Lipopolysaccharides - toxicity Magnetic Resonance Imaging Magnetite Nanoparticles - analysis Magnetite Nanoparticles - chemistry Mannans - metabolism Mannose-Binding Lectins - chemistry Mannose-Binding Lectins - metabolism Medicine Medicine & Public Health Neutrophil Activation - drug effects Neutrophil Activation - physiology Neutrophils - chemistry Neutrophils - cytology Neutrophils - metabolism Radiology Receptors, Cell Surface - chemistry Receptors, Cell Surface - metabolism Research Article |
| title | MR Detection of LPS-Induced Neutrophil Activation using Mannan-Coated Superparamagnetic Iron Oxide Nanoparticles |
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