Phagocytic and Oxidative Burst Activity of Neutrophils in Patients With Spinal Cord Injury

Abstract Objective To evaluate phagocytic activity and neutrophil oxidative burst functions in patients with spinal cord injury (SCI) because alterations in neutrophil metabolic activity can be one of the causes of immune mechanism damage contributing to repeated bacterial infections. Design A contr...

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Veröffentlicht in:Archives of physical medicine and rehabilitation 2013-02, Vol.94 (2), p.369-374
Hauptverfasser: Kanyilmaz, Selcen, MD, Hepguler, Simin, MD, Atamaz, Funda C., MD, Gokmen, Nihal M., MD, Ardeniz, Omur, MD, Sin, Aytul, MD
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container_end_page 374
container_issue 2
container_start_page 369
container_title Archives of physical medicine and rehabilitation
container_volume 94
creator Kanyilmaz, Selcen, MD
Hepguler, Simin, MD
Atamaz, Funda C., MD
Gokmen, Nihal M., MD
Ardeniz, Omur, MD
Sin, Aytul, MD
description Abstract Objective To evaluate phagocytic activity and neutrophil oxidative burst functions in patients with spinal cord injury (SCI) because alterations in neutrophil metabolic activity can be one of the causes of immune mechanism damage contributing to repeated bacterial infections. Design A controlled and cross-sectional study. Setting Departments of physical medicine and rehabilitation and immunology. Participants Patients with SCI (N=34) and 28 healthy controls. Interventions Phagocytosis and oxidative burst in whole-blood neutrophils were assessed by flow cytometry. The percentage of phagocytizing cells after in vitro incubation with Escherichia coli , phagocytic activity (mean intensity of fluorescence [MIF]) and the percentage of neutrophiloxidative burst, and the MIF value of the production of reactive oxygen intermediates (ROIs) were analyzed. In addition, clinical assessment including the level of injury, American Spinal Injury Association scores, and functional status were carried out. Main Outcome Measures Not applicable. Results Although the percentage of E. coli phagocytizing neutrophils was not different between groups, the MIF value of absorbed E. coli was significantly lower in patients with SCI than in controls ( P
doi_str_mv 10.1016/j.apmr.2012.09.015
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Design A controlled and cross-sectional study. Setting Departments of physical medicine and rehabilitation and immunology. Participants Patients with SCI (N=34) and 28 healthy controls. Interventions Phagocytosis and oxidative burst in whole-blood neutrophils were assessed by flow cytometry. The percentage of phagocytizing cells after in vitro incubation with Escherichia coli , phagocytic activity (mean intensity of fluorescence [MIF]) and the percentage of neutrophiloxidative burst, and the MIF value of the production of reactive oxygen intermediates (ROIs) were analyzed. In addition, clinical assessment including the level of injury, American Spinal Injury Association scores, and functional status were carried out. Main Outcome Measures Not applicable. Results Although the percentage of E. coli phagocytizing neutrophils was not different between groups, the MIF value of absorbed E. coli was significantly lower in patients with SCI than in controls ( P &lt;.05). The MIF value of ROI production by neutrophils with both stimulator of phorbol 12-myristate 13-acetate and E. coli was significantly higher in patients with SCI ( P &lt;.05). Conclusions In patients with SCI, decreased phagocytic activity of neutrophils may be a result of a regulatory mechanism to minimize the deleterious effects of increased neutrophil burst activity.</description><identifier>ISSN: 0003-9993</identifier><identifier>EISSN: 1532-821X</identifier><identifier>DOI: 10.1016/j.apmr.2012.09.015</identifier><identifier>PMID: 23022452</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; Aged ; Case-Control Studies ; Cross-Sectional Studies ; Escherichia coli ; Female ; Flow Cytometry ; Humans ; Male ; Middle Aged ; Neutrophils ; Neutrophils - immunology ; Neutrophils - metabolism ; Phagocytosis ; Physical Medicine and Rehabilitation ; Reactive Oxygen Species - metabolism ; Rehabilitation ; Respiratory Burst ; Spinal cord injuries ; Spinal Cord Injuries - immunology ; Spinal Cord Injuries - metabolism ; Young Adult</subject><ispartof>Archives of physical medicine and rehabilitation, 2013-02, Vol.94 (2), p.369-374</ispartof><rights>American Congress of Rehabilitation Medicine</rights><rights>2013 American Congress of Rehabilitation Medicine</rights><rights>Copyright © 2013 American Congress of Rehabilitation Medicine. Published by Elsevier Inc. 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Design A controlled and cross-sectional study. Setting Departments of physical medicine and rehabilitation and immunology. Participants Patients with SCI (N=34) and 28 healthy controls. Interventions Phagocytosis and oxidative burst in whole-blood neutrophils were assessed by flow cytometry. The percentage of phagocytizing cells after in vitro incubation with Escherichia coli , phagocytic activity (mean intensity of fluorescence [MIF]) and the percentage of neutrophiloxidative burst, and the MIF value of the production of reactive oxygen intermediates (ROIs) were analyzed. In addition, clinical assessment including the level of injury, American Spinal Injury Association scores, and functional status were carried out. Main Outcome Measures Not applicable. Results Although the percentage of E. coli phagocytizing neutrophils was not different between groups, the MIF value of absorbed E. coli was significantly lower in patients with SCI than in controls ( P &lt;.05). The MIF value of ROI production by neutrophils with both stimulator of phorbol 12-myristate 13-acetate and E. coli was significantly higher in patients with SCI ( P &lt;.05). Conclusions In patients with SCI, decreased phagocytic activity of neutrophils may be a result of a regulatory mechanism to minimize the deleterious effects of increased neutrophil burst activity.</description><subject>Adult</subject><subject>Aged</subject><subject>Case-Control Studies</subject><subject>Cross-Sectional Studies</subject><subject>Escherichia coli</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neutrophils</subject><subject>Neutrophils - immunology</subject><subject>Neutrophils - metabolism</subject><subject>Phagocytosis</subject><subject>Physical Medicine and Rehabilitation</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Rehabilitation</subject><subject>Respiratory Burst</subject><subject>Spinal cord injuries</subject><subject>Spinal Cord Injuries - immunology</subject><subject>Spinal Cord Injuries - metabolism</subject><subject>Young Adult</subject><issn>0003-9993</issn><issn>1532-821X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk1rFTEUhoMo9rb6B1xIlm5mzNd8BESoF62FYgtVFDchk5x4c507MyaZ4vx7M9zqwoWuwoHnfSHPOQg9o6SkhNYv96WeDqFkhLKSyJLQ6gHa0IqzomX0y0O0IYTwQkrJT9BpjPs81hWnj9EJ44QxUbEN-nqz099GsyRvsB4svv7prU7-DvCbOcSEz00efFrw6PAHmFMYp53vI_YDvskcDCnizz7t8O3kB93j7Rgsvhz2c1ieoEdO9xGe3r9n6NO7tx-374ur64vL7flVYYQQqZCONdryCgTwznUGKmDGdk7ItpYtNFwIyVxda8e1s4R2zDne6k7QxjagDT9DL469Uxh_zBCTOvhooO_1AOMcFRW1qEjLK_p_lLWsoo2om4yyI2rCGGMAp6bgDzosihK16ld7tepXq35FpMr6c-j5ff_cHcD-ifz2nYFXRwCykDsPQUWTJRqwPoBJyo7-3_2v_4qb3g_e6P47LBD34xzyEvI_VMwZdbsewLp_ygiRraz5L2wSrAg</recordid><startdate>20130201</startdate><enddate>20130201</enddate><creator>Kanyilmaz, Selcen, MD</creator><creator>Hepguler, Simin, MD</creator><creator>Atamaz, Funda C., MD</creator><creator>Gokmen, Nihal M., MD</creator><creator>Ardeniz, Omur, MD</creator><creator>Sin, Aytul, MD</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>7TK</scope><scope>H94</scope></search><sort><creationdate>20130201</creationdate><title>Phagocytic and Oxidative Burst Activity of Neutrophils in Patients With Spinal Cord Injury</title><author>Kanyilmaz, Selcen, MD ; Hepguler, Simin, MD ; Atamaz, Funda C., MD ; Gokmen, Nihal M., MD ; Ardeniz, Omur, MD ; Sin, Aytul, MD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-9f27ad35e4e3bfbce5e2cdbf498698e734492f66af3afd01b2ff38ab417d7eac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Case-Control Studies</topic><topic>Cross-Sectional Studies</topic><topic>Escherichia coli</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neutrophils</topic><topic>Neutrophils - immunology</topic><topic>Neutrophils - metabolism</topic><topic>Phagocytosis</topic><topic>Physical Medicine and Rehabilitation</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Rehabilitation</topic><topic>Respiratory Burst</topic><topic>Spinal cord injuries</topic><topic>Spinal Cord Injuries - immunology</topic><topic>Spinal Cord Injuries - metabolism</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kanyilmaz, Selcen, MD</creatorcontrib><creatorcontrib>Hepguler, Simin, MD</creatorcontrib><creatorcontrib>Atamaz, Funda C., MD</creatorcontrib><creatorcontrib>Gokmen, Nihal M., MD</creatorcontrib><creatorcontrib>Ardeniz, Omur, MD</creatorcontrib><creatorcontrib>Sin, Aytul, MD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Archives of physical medicine and rehabilitation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kanyilmaz, Selcen, MD</au><au>Hepguler, Simin, MD</au><au>Atamaz, Funda C., MD</au><au>Gokmen, Nihal M., MD</au><au>Ardeniz, Omur, MD</au><au>Sin, Aytul, MD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phagocytic and Oxidative Burst Activity of Neutrophils in Patients With Spinal Cord Injury</atitle><jtitle>Archives of physical medicine and rehabilitation</jtitle><addtitle>Arch Phys Med Rehabil</addtitle><date>2013-02-01</date><risdate>2013</risdate><volume>94</volume><issue>2</issue><spage>369</spage><epage>374</epage><pages>369-374</pages><issn>0003-9993</issn><eissn>1532-821X</eissn><abstract>Abstract Objective To evaluate phagocytic activity and neutrophil oxidative burst functions in patients with spinal cord injury (SCI) because alterations in neutrophil metabolic activity can be one of the causes of immune mechanism damage contributing to repeated bacterial infections. Design A controlled and cross-sectional study. Setting Departments of physical medicine and rehabilitation and immunology. Participants Patients with SCI (N=34) and 28 healthy controls. Interventions Phagocytosis and oxidative burst in whole-blood neutrophils were assessed by flow cytometry. The percentage of phagocytizing cells after in vitro incubation with Escherichia coli , phagocytic activity (mean intensity of fluorescence [MIF]) and the percentage of neutrophiloxidative burst, and the MIF value of the production of reactive oxygen intermediates (ROIs) were analyzed. In addition, clinical assessment including the level of injury, American Spinal Injury Association scores, and functional status were carried out. Main Outcome Measures Not applicable. Results Although the percentage of E. coli phagocytizing neutrophils was not different between groups, the MIF value of absorbed E. coli was significantly lower in patients with SCI than in controls ( P &lt;.05). The MIF value of ROI production by neutrophils with both stimulator of phorbol 12-myristate 13-acetate and E. coli was significantly higher in patients with SCI ( P &lt;.05). Conclusions In patients with SCI, decreased phagocytic activity of neutrophils may be a result of a regulatory mechanism to minimize the deleterious effects of increased neutrophil burst activity.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>23022452</pmid><doi>10.1016/j.apmr.2012.09.015</doi><tpages>6</tpages></addata></record>
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subjects Adult
Aged
Case-Control Studies
Cross-Sectional Studies
Escherichia coli
Female
Flow Cytometry
Humans
Male
Middle Aged
Neutrophils
Neutrophils - immunology
Neutrophils - metabolism
Phagocytosis
Physical Medicine and Rehabilitation
Reactive Oxygen Species - metabolism
Rehabilitation
Respiratory Burst
Spinal cord injuries
Spinal Cord Injuries - immunology
Spinal Cord Injuries - metabolism
Young Adult
title Phagocytic and Oxidative Burst Activity of Neutrophils in Patients With Spinal Cord Injury
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