Role of recombinant plasmid pEGFP-N1-IGF-1 transfection in alleviating osteoporosis in ovariectomized rats
Decreased levels of serum insulin-like growth factor-1 (IGF-1) have been proven to cause osteoporosis. Gene transfer of IGF-1 offers an attractive technology to treat skeletal metabolic disorders including osteoporosis, but the viral vectors are limited by their high antigenicity and immune response...
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| Veröffentlicht in: | Journal of molecular histology 2013-10, Vol.44 (5), p.535-544 |
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| creator | Liu, Hai Chun Zhao, Hua Chen, Jian Wu, Wen Liang Wang, Hong Liang Jiao, Gang Jun Chen, Yun Zhen |
| description | Decreased levels of serum insulin-like growth factor-1 (IGF-1) have been proven to cause osteoporosis. Gene transfer of IGF-1 offers an attractive technology to treat skeletal metabolic disorders including osteoporosis, but the viral vectors are limited by their high antigenicity and immune response. Our purpose was to investigate the expression of a non-invasive vector, recombinant plasmid enhanced green fluorescent protein-N1 (pEGFP-N1) that transferred IGF-1 gene into ovariectomized (OVX) rats in vivo and evaluate the effect of this therapy on osteoporosis. OVX or sham operations were performed in 60 female, 7-month-old unmated SD rats. 12 weeks after OVX operation, the vectors were transfected to the 10-month-old rats and experimental data were detected from 48 h to 7 week after transfection. Our results showed that remarkable expression of fluorescence and serum IGF-1 was observed in the rats transfected by recombinant plasmids, indicating that IGF-1 gene was successfully transferred to OVX rats by injecting the vector through hydrodynamic method via the tail vein. The bone metabolism index including serum alkaline phosphatase, the histomorphometric parameters of lumbar vertebra including trabecular area percentage, trabecular thickness, trabecular number and trabecular separation, and the bone mineral density (BMD) and biomechanical parameters of lumbar vertebra including BMD, maximum condensing force, crushing strength in OVX rats transfected by pEGFP-N1-IGF-1 were improved remarkably compared with OVX+pEGFP-N1 rats, indicating that the transfection of recombinant plasmid pEGFP-N1-IGF-1 played a significant role in alleviating osteoporosis in rats induced by OVX. This encouraged a potential approach of IGF-1 gene therapy to the treatment of osteoporosis. |
| doi_str_mv | 10.1007/s10735-013-9498-3 |
| format | Article |
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Gene transfer of IGF-1 offers an attractive technology to treat skeletal metabolic disorders including osteoporosis, but the viral vectors are limited by their high antigenicity and immune response. Our purpose was to investigate the expression of a non-invasive vector, recombinant plasmid enhanced green fluorescent protein-N1 (pEGFP-N1) that transferred IGF-1 gene into ovariectomized (OVX) rats in vivo and evaluate the effect of this therapy on osteoporosis. OVX or sham operations were performed in 60 female, 7-month-old unmated SD rats. 12 weeks after OVX operation, the vectors were transfected to the 10-month-old rats and experimental data were detected from 48 h to 7 week after transfection. Our results showed that remarkable expression of fluorescence and serum IGF-1 was observed in the rats transfected by recombinant plasmids, indicating that IGF-1 gene was successfully transferred to OVX rats by injecting the vector through hydrodynamic method via the tail vein. The bone metabolism index including serum alkaline phosphatase, the histomorphometric parameters of lumbar vertebra including trabecular area percentage, trabecular thickness, trabecular number and trabecular separation, and the bone mineral density (BMD) and biomechanical parameters of lumbar vertebra including BMD, maximum condensing force, crushing strength in OVX rats transfected by pEGFP-N1-IGF-1 were improved remarkably compared with OVX+pEGFP-N1 rats, indicating that the transfection of recombinant plasmid pEGFP-N1-IGF-1 played a significant role in alleviating osteoporosis in rats induced by OVX. This encouraged a potential approach of IGF-1 gene therapy to the treatment of osteoporosis.</description><identifier>ISSN: 1567-2379</identifier><identifier>EISSN: 1567-2387</identifier><identifier>DOI: 10.1007/s10735-013-9498-3</identifier><identifier>PMID: 23508541</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Alkaline phosphatase ; Alkaline Phosphatase - blood ; Alkaline Phosphatase - genetics ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Bone Density - genetics ; Cell Biology ; Developmental Biology ; Female ; Genetic Therapy - methods ; Green Fluorescent Proteins - blood ; Green Fluorescent Proteins - genetics ; Injections, Intravenous ; Insulin-Like Growth Factor I - genetics ; Insulin-Like Growth Factor I - metabolism ; Life Sciences ; Lumbar Vertebrae - metabolism ; Lumbar Vertebrae - pathology ; Original Paper ; Osteoporosis - genetics ; Osteoporosis - pathology ; Osteoporosis - therapy ; Ovariectomy ; Plasmids ; Rats ; Rats, Sprague-Dawley ; Recombinant Fusion Proteins - blood ; Recombinant Fusion Proteins - genetics ; Transfection</subject><ispartof>Journal of molecular histology, 2013-10, Vol.44 (5), p.535-544</ispartof><rights>Springer Science+Business Media Dordrecht 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c541t-8c61ccad8e8a2b493ff118d7d9f68f94543aaf9fbae98dd691e67282d5136513</citedby><cites>FETCH-LOGICAL-c541t-8c61ccad8e8a2b493ff118d7d9f68f94543aaf9fbae98dd691e67282d5136513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10735-013-9498-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10735-013-9498-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27928,27929,41492,42561,51323</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23508541$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Hai Chun</creatorcontrib><creatorcontrib>Zhao, Hua</creatorcontrib><creatorcontrib>Chen, Jian</creatorcontrib><creatorcontrib>Wu, Wen Liang</creatorcontrib><creatorcontrib>Wang, Hong Liang</creatorcontrib><creatorcontrib>Jiao, Gang Jun</creatorcontrib><creatorcontrib>Chen, Yun Zhen</creatorcontrib><title>Role of recombinant plasmid pEGFP-N1-IGF-1 transfection in alleviating osteoporosis in ovariectomized rats</title><title>Journal of molecular histology</title><addtitle>J Mol Hist</addtitle><addtitle>J Mol Histol</addtitle><description>Decreased levels of serum insulin-like growth factor-1 (IGF-1) have been proven to cause osteoporosis. Gene transfer of IGF-1 offers an attractive technology to treat skeletal metabolic disorders including osteoporosis, but the viral vectors are limited by their high antigenicity and immune response. Our purpose was to investigate the expression of a non-invasive vector, recombinant plasmid enhanced green fluorescent protein-N1 (pEGFP-N1) that transferred IGF-1 gene into ovariectomized (OVX) rats in vivo and evaluate the effect of this therapy on osteoporosis. OVX or sham operations were performed in 60 female, 7-month-old unmated SD rats. 12 weeks after OVX operation, the vectors were transfected to the 10-month-old rats and experimental data were detected from 48 h to 7 week after transfection. Our results showed that remarkable expression of fluorescence and serum IGF-1 was observed in the rats transfected by recombinant plasmids, indicating that IGF-1 gene was successfully transferred to OVX rats by injecting the vector through hydrodynamic method via the tail vein. The bone metabolism index including serum alkaline phosphatase, the histomorphometric parameters of lumbar vertebra including trabecular area percentage, trabecular thickness, trabecular number and trabecular separation, and the bone mineral density (BMD) and biomechanical parameters of lumbar vertebra including BMD, maximum condensing force, crushing strength in OVX rats transfected by pEGFP-N1-IGF-1 were improved remarkably compared with OVX+pEGFP-N1 rats, indicating that the transfection of recombinant plasmid pEGFP-N1-IGF-1 played a significant role in alleviating osteoporosis in rats induced by OVX. This encouraged a potential approach of IGF-1 gene therapy to the treatment of osteoporosis.</description><subject>Alkaline phosphatase</subject><subject>Alkaline Phosphatase - blood</subject><subject>Alkaline Phosphatase - genetics</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bone Density - genetics</subject><subject>Cell Biology</subject><subject>Developmental Biology</subject><subject>Female</subject><subject>Genetic Therapy - methods</subject><subject>Green Fluorescent Proteins - blood</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Injections, Intravenous</subject><subject>Insulin-Like Growth Factor I - genetics</subject><subject>Insulin-Like Growth Factor I - metabolism</subject><subject>Life Sciences</subject><subject>Lumbar Vertebrae - metabolism</subject><subject>Lumbar Vertebrae - pathology</subject><subject>Original Paper</subject><subject>Osteoporosis - genetics</subject><subject>Osteoporosis - pathology</subject><subject>Osteoporosis - therapy</subject><subject>Ovariectomy</subject><subject>Plasmids</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Recombinant Fusion Proteins - blood</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Transfection</subject><issn>1567-2379</issn><issn>1567-2387</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkU2LFDEQhoMo7rr6A7xIwIuXaCpJ5-Moy864sKjI3kOmkywZupM26VnQX2-GWRcRBA9FCuqptyr1IvQa6HugVH1oQBUfCAVOjDCa8CfoHAapCONaPX3MlTlDL1rbU8q0FOY5OmN8oHoQcI7238oUcIm4hrHMu5RdXvEyuTYnj5er7eYr-QzkershgNfqcothXFPJOGXspincJ7emfIdLW0NZSi0ttWOt3LuaOlrm9DN4XN3aXqJn0U0tvHp4L9Dt5ur28hO5-bK9vvx4Q8a-0Ur0KGEcnddBO7YThscIoL3yJkodjRgEdy6auHPBaO-lgSAV08wPwGWPC_TuJLvU8v0Q2mrn1MYwTS6HcmgWhBT9WJrp_0AF51qCkR19-xe6L4ea-z86xZXigtHjbDhRYz9EqyHapabZ1R8WqD1aZk-W2c7ao2WW9543D8qH3Rz8Y8dvjzrATkDrpXwX6h-j_6n6C3CqoMQ</recordid><startdate>20131001</startdate><enddate>20131001</enddate><creator>Liu, Hai Chun</creator><creator>Zhao, Hua</creator><creator>Chen, Jian</creator><creator>Wu, Wen Liang</creator><creator>Wang, Hong Liang</creator><creator>Jiao, Gang Jun</creator><creator>Chen, Yun Zhen</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20131001</creationdate><title>Role of recombinant plasmid pEGFP-N1-IGF-1 transfection in alleviating osteoporosis in ovariectomized rats</title><author>Liu, Hai Chun ; Zhao, Hua ; Chen, Jian ; Wu, Wen Liang ; Wang, Hong Liang ; Jiao, Gang Jun ; Chen, Yun Zhen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c541t-8c61ccad8e8a2b493ff118d7d9f68f94543aaf9fbae98dd691e67282d5136513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Alkaline phosphatase</topic><topic>Alkaline Phosphatase - blood</topic><topic>Alkaline Phosphatase - genetics</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bone Density - genetics</topic><topic>Cell Biology</topic><topic>Developmental Biology</topic><topic>Female</topic><topic>Genetic Therapy - methods</topic><topic>Green Fluorescent Proteins - blood</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Injections, Intravenous</topic><topic>Insulin-Like Growth Factor I - genetics</topic><topic>Insulin-Like Growth Factor I - metabolism</topic><topic>Life Sciences</topic><topic>Lumbar Vertebrae - metabolism</topic><topic>Lumbar Vertebrae - pathology</topic><topic>Original Paper</topic><topic>Osteoporosis - genetics</topic><topic>Osteoporosis - pathology</topic><topic>Osteoporosis - therapy</topic><topic>Ovariectomy</topic><topic>Plasmids</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Recombinant Fusion Proteins - blood</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Hai Chun</creatorcontrib><creatorcontrib>Zhao, Hua</creatorcontrib><creatorcontrib>Chen, Jian</creatorcontrib><creatorcontrib>Wu, Wen Liang</creatorcontrib><creatorcontrib>Wang, Hong Liang</creatorcontrib><creatorcontrib>Jiao, Gang Jun</creatorcontrib><creatorcontrib>Chen, Yun Zhen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of molecular histology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Hai Chun</au><au>Zhao, Hua</au><au>Chen, Jian</au><au>Wu, Wen Liang</au><au>Wang, Hong Liang</au><au>Jiao, Gang Jun</au><au>Chen, Yun Zhen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of recombinant plasmid pEGFP-N1-IGF-1 transfection in alleviating osteoporosis in ovariectomized rats</atitle><jtitle>Journal of molecular histology</jtitle><stitle>J Mol Hist</stitle><addtitle>J Mol Histol</addtitle><date>2013-10-01</date><risdate>2013</risdate><volume>44</volume><issue>5</issue><spage>535</spage><epage>544</epage><pages>535-544</pages><issn>1567-2379</issn><eissn>1567-2387</eissn><abstract>Decreased levels of serum insulin-like growth factor-1 (IGF-1) have been proven to cause osteoporosis. Gene transfer of IGF-1 offers an attractive technology to treat skeletal metabolic disorders including osteoporosis, but the viral vectors are limited by their high antigenicity and immune response. Our purpose was to investigate the expression of a non-invasive vector, recombinant plasmid enhanced green fluorescent protein-N1 (pEGFP-N1) that transferred IGF-1 gene into ovariectomized (OVX) rats in vivo and evaluate the effect of this therapy on osteoporosis. OVX or sham operations were performed in 60 female, 7-month-old unmated SD rats. 12 weeks after OVX operation, the vectors were transfected to the 10-month-old rats and experimental data were detected from 48 h to 7 week after transfection. Our results showed that remarkable expression of fluorescence and serum IGF-1 was observed in the rats transfected by recombinant plasmids, indicating that IGF-1 gene was successfully transferred to OVX rats by injecting the vector through hydrodynamic method via the tail vein. The bone metabolism index including serum alkaline phosphatase, the histomorphometric parameters of lumbar vertebra including trabecular area percentage, trabecular thickness, trabecular number and trabecular separation, and the bone mineral density (BMD) and biomechanical parameters of lumbar vertebra including BMD, maximum condensing force, crushing strength in OVX rats transfected by pEGFP-N1-IGF-1 were improved remarkably compared with OVX+pEGFP-N1 rats, indicating that the transfection of recombinant plasmid pEGFP-N1-IGF-1 played a significant role in alleviating osteoporosis in rats induced by OVX. This encouraged a potential approach of IGF-1 gene therapy to the treatment of osteoporosis.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>23508541</pmid><doi>10.1007/s10735-013-9498-3</doi><tpages>10</tpages></addata></record> |
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| subjects | Alkaline phosphatase Alkaline Phosphatase - blood Alkaline Phosphatase - genetics Animals Biomedical and Life Sciences Biomedicine Bone Density - genetics Cell Biology Developmental Biology Female Genetic Therapy - methods Green Fluorescent Proteins - blood Green Fluorescent Proteins - genetics Injections, Intravenous Insulin-Like Growth Factor I - genetics Insulin-Like Growth Factor I - metabolism Life Sciences Lumbar Vertebrae - metabolism Lumbar Vertebrae - pathology Original Paper Osteoporosis - genetics Osteoporosis - pathology Osteoporosis - therapy Ovariectomy Plasmids Rats Rats, Sprague-Dawley Recombinant Fusion Proteins - blood Recombinant Fusion Proteins - genetics Transfection |
| title | Role of recombinant plasmid pEGFP-N1-IGF-1 transfection in alleviating osteoporosis in ovariectomized rats |
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