Multistep DNA-Templated Synthesis Using a Universal Template
We report a DNA-templated synthesis method that allows construction of the entire DNA-encoded library with a single DNA template. Taking advantage of deoxyinosine’s indiscriminate base-pairing property, we designed a “universal template” that is capable of directing chemical reactions with multiple...
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| Veröffentlicht in: | Journal of the American Chemical Society 2013-11, Vol.135 (47), p.17727-17730 |
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| container_end_page | 17730 |
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| container_issue | 47 |
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| container_title | Journal of the American Chemical Society |
| container_volume | 135 |
| creator | Li, Yizhou Zhao, Peng Zhang, Mingda Zhao, Xianyuan Li, Xiaoyu |
| description | We report a DNA-templated synthesis method that allows construction of the entire DNA-encoded library with a single DNA template. Taking advantage of deoxyinosine’s indiscriminate base-pairing property, we designed a “universal template” that is capable of directing chemical reactions with multiple reactant DNAs with different sequences. In combination with other design features including photocleavable linkers and direct encoding by the reactant DNA, we demonstrated the capabilities of the universal template in library synthesis, target selection, and hit decoding. Our method can be generally and straightforwardly applied to prepare a variety of chemically diverse DNA-encoded libraries. |
| doi_str_mv | 10.1021/ja409936r |
| format | Article |
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| ispartof | Journal of the American Chemical Society, 2013-11, Vol.135 (47), p.17727-17730 |
| issn | 0002-7863 1520-5126 |
| language | eng |
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| source | MEDLINE; ACS Publications |
| subjects | Base Pairing Base Sequence DNA - chemical synthesis DNA - chemistry Gene Library Molecular Sequence Data |
| title | Multistep DNA-Templated Synthesis Using a Universal Template |
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