Transplants of embryonic brainstem containing the locus coeruleus into spinal cord enhance the hindlimb flexion reflex in adult rats
Cell suspensions of embryonic brainstem containing the locus coeruleus were injected intervertebrally into the lumbar spinal cord of adult rats whose descending catecholamine (CA) fibers had been lesioned with intracisternal injection of 6-hydroxydopamine. Up to 1100 CA cells were found 2 and 4 mont...
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| Veröffentlicht in: | Brain research 1986-09, Vol.381 (2), p.225-236 |
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| creator | Buchanan, James T. Nornes, Howard O. |
| description | Cell suspensions of embryonic brainstem containing the locus coeruleus were injected intervertebrally into the lumbar spinal cord of adult rats whose descending catecholamine (CA) fibers had been lesioned with intracisternal injection of 6-hydroxydopamine. Up to 1100 CA cells were found 2 and 4 months later, and these cells grew processes which produced histologically detectable reinnervation of the lumbar gray matter on the injected side of the cord. To assess the functional activity of the transplanted CA cells, the force of the hindlimb flexion reflex was measured in acute spinal rats. This reflex has been shown previously to be strongly enhanced by catecholamines. The flexion reflexes were significantly stronger in the transplanted rats than in the controls. Further, the flexion reflexes were significantly reduced by phenoxybenzamine, an α-adrenergic blocker, in the transplanted rats while the reflexes of controls were not significantly changed. These results demonstrate that cell suspension transplants of embryonic brainstem containing the locus coeruleus into the adult rat spinal cord survive, grow reinnervating catecholamine processes, and can affect the functional activity of the spinal cord. |
| doi_str_mv | 10.1016/0006-8993(86)90071-5 |
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Up to 1100 CA cells were found 2 and 4 months later, and these cells grew processes which produced histologically detectable reinnervation of the lumbar gray matter on the injected side of the cord. To assess the functional activity of the transplanted CA cells, the force of the hindlimb flexion reflex was measured in acute spinal rats. This reflex has been shown previously to be strongly enhanced by catecholamines. The flexion reflexes were significantly stronger in the transplanted rats than in the controls. Further, the flexion reflexes were significantly reduced by phenoxybenzamine, an α-adrenergic blocker, in the transplanted rats while the reflexes of controls were not significantly changed. These results demonstrate that cell suspension transplants of embryonic brainstem containing the locus coeruleus into the adult rat spinal cord survive, grow reinnervating catecholamine processes, and can affect the functional activity of the spinal cord.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/0006-8993(86)90071-5</identifier><identifier>PMID: 3092991</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>6-hydroxydopamine ; Animals ; Biological and medical sciences ; brainstem ; catecholamine reinnervation ; Catecholamines - metabolism ; cell suspension ; Female ; Fundamental and applied biological sciences. Psychology ; General aspects. Models. Methods ; Hindlimb - innervation ; hindlimb flexion reflex ; Hydroxydopamines ; locus coeruleus ; Locus Coeruleus - transplantation ; lumbar spinal cord ; neural transplant ; Oxidopamine ; Rats ; Rats, Inbred Strains ; Reflex, Abnormal - therapy ; Spinal Cord - metabolism ; Spinal Cord Diseases - chemically induced ; Spinal Cord Diseases - metabolism ; Spinal Cord Diseases - therapy ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research, 1986-09, Vol.381 (2), p.225-236</ispartof><rights>1986 Elsevier Science Publishers B.V. (Biomedical Division)</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-997328f57970fbbacddfad37a9aa2028c20de23f76068659fc35633a519e41bd3</citedby><cites>FETCH-LOGICAL-c332t-997328f57970fbbacddfad37a9aa2028c20de23f76068659fc35633a519e41bd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-8993(86)90071-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7911485$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3092991$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Buchanan, James T.</creatorcontrib><creatorcontrib>Nornes, Howard O.</creatorcontrib><title>Transplants of embryonic brainstem containing the locus coeruleus into spinal cord enhance the hindlimb flexion reflex in adult rats</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>Cell suspensions of embryonic brainstem containing the locus coeruleus were injected intervertebrally into the lumbar spinal cord of adult rats whose descending catecholamine (CA) fibers had been lesioned with intracisternal injection of 6-hydroxydopamine. Up to 1100 CA cells were found 2 and 4 months later, and these cells grew processes which produced histologically detectable reinnervation of the lumbar gray matter on the injected side of the cord. To assess the functional activity of the transplanted CA cells, the force of the hindlimb flexion reflex was measured in acute spinal rats. This reflex has been shown previously to be strongly enhanced by catecholamines. The flexion reflexes were significantly stronger in the transplanted rats than in the controls. Further, the flexion reflexes were significantly reduced by phenoxybenzamine, an α-adrenergic blocker, in the transplanted rats while the reflexes of controls were not significantly changed. These results demonstrate that cell suspension transplants of embryonic brainstem containing the locus coeruleus into the adult rat spinal cord survive, grow reinnervating catecholamine processes, and can affect the functional activity of the spinal cord.</description><subject>6-hydroxydopamine</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>brainstem</subject><subject>catecholamine reinnervation</subject><subject>Catecholamines - metabolism</subject><subject>cell suspension</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects. Models. Methods</subject><subject>Hindlimb - innervation</subject><subject>hindlimb flexion reflex</subject><subject>Hydroxydopamines</subject><subject>locus coeruleus</subject><subject>Locus Coeruleus - transplantation</subject><subject>lumbar spinal cord</subject><subject>neural transplant</subject><subject>Oxidopamine</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Reflex, Abnormal - therapy</subject><subject>Spinal Cord - metabolism</subject><subject>Spinal Cord Diseases - chemically induced</subject><subject>Spinal Cord Diseases - metabolism</subject><subject>Spinal Cord Diseases - therapy</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE-PFCEQxYnRrLOr30ATDsbooZU_3XRzMTEbV0028bKeCQ2Fg6FhBNq4dz-49M5kjp54VL16qfoh9IKSd5RQ8Z4QIrpJSv5mEm8lISPthkdoR6eRdYL15DHanS1P0WUpP9uXc0ku0AUnkklJd-jvXdaxHIKOteDkMCxzvk_RGzxn7WOpsGCTYm3axx-47gGHZNbSipDXAE35WBMuBx91aNVsMcS9jgYezHsfbfDLjF2APz5FnGFTbQhru4aKs67lGXridCjw_PReoe83n-6uv3S33z5_vf542xnOWe2kHDmb3DDKkbh51sZapy0ftdSaETYZRiww7kZBxCQG6QwfBOd6oBJ6Olt-hV4fcw85_VqhVLX4YiC06yGtRdFeMDYx3oz90WhyKqWtrA7ZLzrfK0rUBl9tZNVGVk1CPcBXQxt7ecpf5wXseehEu_Vfnfq6GB1cQ298OdtGSWk_bTEfjjZoLH57yKoYD42o9RlMVTb5_-_xDyHOovU</recordid><startdate>19860903</startdate><enddate>19860903</enddate><creator>Buchanan, James T.</creator><creator>Nornes, Howard O.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>19860903</creationdate><title>Transplants of embryonic brainstem containing the locus coeruleus into spinal cord enhance the hindlimb flexion reflex in adult rats</title><author>Buchanan, James T. ; Nornes, Howard O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-997328f57970fbbacddfad37a9aa2028c20de23f76068659fc35633a519e41bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>6-hydroxydopamine</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>brainstem</topic><topic>catecholamine reinnervation</topic><topic>Catecholamines - metabolism</topic><topic>cell suspension</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects. Models. Methods</topic><topic>Hindlimb - innervation</topic><topic>hindlimb flexion reflex</topic><topic>Hydroxydopamines</topic><topic>locus coeruleus</topic><topic>Locus Coeruleus - transplantation</topic><topic>lumbar spinal cord</topic><topic>neural transplant</topic><topic>Oxidopamine</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Reflex, Abnormal - therapy</topic><topic>Spinal Cord - metabolism</topic><topic>Spinal Cord Diseases - chemically induced</topic><topic>Spinal Cord Diseases - metabolism</topic><topic>Spinal Cord Diseases - therapy</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Buchanan, James T.</creatorcontrib><creatorcontrib>Nornes, Howard O.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Buchanan, James T.</au><au>Nornes, Howard O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transplants of embryonic brainstem containing the locus coeruleus into spinal cord enhance the hindlimb flexion reflex in adult rats</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1986-09-03</date><risdate>1986</risdate><volume>381</volume><issue>2</issue><spage>225</spage><epage>236</epage><pages>225-236</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>Cell suspensions of embryonic brainstem containing the locus coeruleus were injected intervertebrally into the lumbar spinal cord of adult rats whose descending catecholamine (CA) fibers had been lesioned with intracisternal injection of 6-hydroxydopamine. Up to 1100 CA cells were found 2 and 4 months later, and these cells grew processes which produced histologically detectable reinnervation of the lumbar gray matter on the injected side of the cord. To assess the functional activity of the transplanted CA cells, the force of the hindlimb flexion reflex was measured in acute spinal rats. This reflex has been shown previously to be strongly enhanced by catecholamines. The flexion reflexes were significantly stronger in the transplanted rats than in the controls. Further, the flexion reflexes were significantly reduced by phenoxybenzamine, an α-adrenergic blocker, in the transplanted rats while the reflexes of controls were not significantly changed. These results demonstrate that cell suspension transplants of embryonic brainstem containing the locus coeruleus into the adult rat spinal cord survive, grow reinnervating catecholamine processes, and can affect the functional activity of the spinal cord.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>3092991</pmid><doi>10.1016/0006-8993(86)90071-5</doi><tpages>12</tpages></addata></record> |
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| ispartof | Brain research, 1986-09, Vol.381 (2), p.225-236 |
| issn | 0006-8993 1872-6240 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | 6-hydroxydopamine Animals Biological and medical sciences brainstem catecholamine reinnervation Catecholamines - metabolism cell suspension Female Fundamental and applied biological sciences. Psychology General aspects. Models. Methods Hindlimb - innervation hindlimb flexion reflex Hydroxydopamines locus coeruleus Locus Coeruleus - transplantation lumbar spinal cord neural transplant Oxidopamine Rats Rats, Inbred Strains Reflex, Abnormal - therapy Spinal Cord - metabolism Spinal Cord Diseases - chemically induced Spinal Cord Diseases - metabolism Spinal Cord Diseases - therapy Vertebrates: nervous system and sense organs |
| title | Transplants of embryonic brainstem containing the locus coeruleus into spinal cord enhance the hindlimb flexion reflex in adult rats |
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