Bean lectins. IV. Genetic variation in the non-denatured structure of lectins from different Phaseolus vulgaris L. cultivars

Variation in the native conformation of bean lectins was examined using electrophoresis of non-denatured total protein extracts and purified albumin and globulin lectin. The observed variation was related to the genetic variation reported previously for lectin polypeptide composition as revealed by...

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Veröffentlicht in:Theoretical and applied genetics 1984-04, Vol.67 (6), p.547-552
Hauptverfasser: Osborn, T.C, Manen, J.F, Brown, J.W.S, Bliss, F.A
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Bliss, F.A
description Variation in the native conformation of bean lectins was examined using electrophoresis of non-denatured total protein extracts and purified albumin and globulin lectin. The observed variation was related to the genetic variation reported previously for lectin polypeptide composition as revealed by two-dimensional isoelectricfocusing-sodium dodecyl sulfate polyacrylamide gel electrophoresis (IEF-SDS/PAGE). When eleven cultivars with different IEF-SDS/PAGE lectin polypeptide compositions were compared, eight had unique non-denatured lectin patterns and three had identical patterns. For some cultivars differences in non-denatured lectin patterns were observed between the purified albumin and globulin lectin preparations.
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subjects Phaseolus vulgaris
plant breeding
plant genetics
title Bean lectins. IV. Genetic variation in the non-denatured structure of lectins from different Phaseolus vulgaris L. cultivars
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