Bean lectins. IV. Genetic variation in the non-denatured structure of lectins from different Phaseolus vulgaris L. cultivars
Variation in the native conformation of bean lectins was examined using electrophoresis of non-denatured total protein extracts and purified albumin and globulin lectin. The observed variation was related to the genetic variation reported previously for lectin polypeptide composition as revealed by...
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Veröffentlicht in: | Theoretical and applied genetics 1984-04, Vol.67 (6), p.547-552 |
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creator | Osborn, T.C Manen, J.F Brown, J.W.S Bliss, F.A |
description | Variation in the native conformation of bean lectins was examined using electrophoresis of non-denatured total protein extracts and purified albumin and globulin lectin. The observed variation was related to the genetic variation reported previously for lectin polypeptide composition as revealed by two-dimensional isoelectricfocusing-sodium dodecyl sulfate polyacrylamide gel electrophoresis (IEF-SDS/PAGE). When eleven cultivars with different IEF-SDS/PAGE lectin polypeptide compositions were compared, eight had unique non-denatured lectin patterns and three had identical patterns. For some cultivars differences in non-denatured lectin patterns were observed between the purified albumin and globulin lectin preparations. |
doi_str_mv | 10.1007/BF00264902 |
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IV. Genetic variation in the non-denatured structure of lectins from different Phaseolus vulgaris L. cultivars</title><title>Theoretical and applied genetics</title><addtitle>Theor Appl Genet</addtitle><description>Variation in the native conformation of bean lectins was examined using electrophoresis of non-denatured total protein extracts and purified albumin and globulin lectin. The observed variation was related to the genetic variation reported previously for lectin polypeptide composition as revealed by two-dimensional isoelectricfocusing-sodium dodecyl sulfate polyacrylamide gel electrophoresis (IEF-SDS/PAGE). When eleven cultivars with different IEF-SDS/PAGE lectin polypeptide compositions were compared, eight had unique non-denatured lectin patterns and three had identical patterns. For some cultivars differences in non-denatured lectin patterns were observed between the purified albumin and globulin lectin preparations.</description><subject>Phaseolus vulgaris</subject><subject>plant breeding</subject><subject>plant genetics</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><recordid>eNp90U9LHDEcxvFQWnSrXvoC2pxKKcyav5PMsYpaYaGFqtchmfyiKbOJTTKC0BffyGp76ymBfPge8iD0jpI1JUQdn5wTwnoxEPYKrajgrGNMsNdoRYggnVSS7aO3pfwkjUnC99B-e5ZaC7lCv0_ARDzDVEMsa3x5s8YXEKGGCT-YHEwNKeIQcb0DHFPsHERTlwwOl5qX6emKk38JYJ_TFrvgPWSIFX-_MwXSvBT8sMy3rVfwZo2nZa6h1csheuPNXODo-TxA1-dnV6dfu823i8vTL5tuYlLWTg-8d8Iaqa21mg1CCqqBq144IoyyjjnlTU8VU1Y4owRYbomavNOMOcn4Afq0697n9GuBUsdtKBPMs4mQljJS0VOtJRlEox__T7nWvB9Ig593cMqplAx-vM9ha_LjSMn4NMv4b5aG3z9XF7sF95e-7NDAhx3wJo3mtn3UeP2DEcrbYoIyrfgfHd6Q-Q</recordid><startdate>198404</startdate><enddate>198404</enddate><creator>Osborn, T.C</creator><creator>Manen, J.F</creator><creator>Brown, J.W.S</creator><creator>Bliss, F.A</creator><scope>FBQ</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>198404</creationdate><title>Bean lectins. 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Genetic variation in the non-denatured structure of lectins from different Phaseolus vulgaris L. cultivars</title><author>Osborn, T.C ; Manen, J.F ; Brown, J.W.S ; Bliss, F.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c255t-8936d4ba58bbb82945418e3764d04a7bd2d7fa61727b4da74eb3b07cfd822d523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Phaseolus vulgaris</topic><topic>plant breeding</topic><topic>plant genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Osborn, T.C</creatorcontrib><creatorcontrib>Manen, J.F</creatorcontrib><creatorcontrib>Brown, J.W.S</creatorcontrib><creatorcontrib>Bliss, F.A</creatorcontrib><collection>AGRIS</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Theoretical and applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Osborn, T.C</au><au>Manen, J.F</au><au>Brown, J.W.S</au><au>Bliss, F.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bean lectins. 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For some cultivars differences in non-denatured lectin patterns were observed between the purified albumin and globulin lectin preparations.</abstract><cop>Germany</cop><pmid>24258845</pmid><doi>10.1007/BF00264902</doi><tpages>6</tpages></addata></record> |
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subjects | Phaseolus vulgaris plant breeding plant genetics |
title | Bean lectins. IV. Genetic variation in the non-denatured structure of lectins from different Phaseolus vulgaris L. cultivars |
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