Control of Photosystem II in spinach leaves by continuous light and by light pulses given in the dark
The light-induced induction of components of non-photochemical quenching of chlorophyll fluorescence which are distinguished by different rates of dark relaxation (qNf, rapidly relaxing and qNs, slowly relaxing or not relaxing at all in the presence brief saturating light pulses which interrupt dark...
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Veröffentlicht in: | Photosynthesis research 1996-11, Vol.50 (2), p.181-191 |
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description | The light-induced induction of components of non-photochemical quenching of chlorophyll fluorescence which are distinguished by different rates of dark relaxation (qNf, rapidly relaxing and qNs, slowly relaxing or not relaxing at all in the presence brief saturating light pulses which interrupt darkness at low frequencies) was studied in leaves of spinach.After dark adaptation of the leaves, a fast relaxing component developed in low light only after a lag phase. Quenching increased towards a maximum with increasing photon flux density. This 'fast' component of quenching was identified as energy-dependent quenching qE. It required formation of an appreciable transthylakoid ΔpH and was insignificant when darkened spinach leaves received 1 s pulses of light every 30 s even though zeaxanthin was formed from violaxanthin under these conditions.Another quenching component termed qNs developed in low light without a lag phase. It was not dependent on a transthylakoid pH gradient, decayed exponentially with a long half time of relaxation and was about 20% of total quenching irrespective of light intensity. When darkened leaves were flashed at frequencies higher than 0.004 Hz with 1 s light pulses, this quenching also appeared. Its extent was very considerable, and it did not require formation of zeaxanthin. Relaxation was accelerated by far-red light, and this acceleration was abolished by NaF.We suggest that qNs is the result of a so-called state transition, in which LHC II moves after its phosphorylation from fluorescent PS II to nonfluorescent PS I. This state transition was capable of decreasing in darkened leaves the potential maximum quantum efficiency of electron flow through Photosystem II by about 20%. |
doi_str_mv | 10.1007/BF00014888 |
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(Wuerzburg Univ. (Germany). Julius-von-Sachs-Inst. of Biological Sciences) ; Wiese, C ; Neimanis, S ; Heber, U</creator><creatorcontrib>Bukhov, N.G. (Wuerzburg Univ. (Germany). Julius-von-Sachs-Inst. of Biological Sciences) ; Wiese, C ; Neimanis, S ; Heber, U</creatorcontrib><description>The light-induced induction of components of non-photochemical quenching of chlorophyll fluorescence which are distinguished by different rates of dark relaxation (qNf, rapidly relaxing and qNs, slowly relaxing or not relaxing at all in the presence brief saturating light pulses which interrupt darkness at low frequencies) was studied in leaves of spinach.After dark adaptation of the leaves, a fast relaxing component developed in low light only after a lag phase. Quenching increased towards a maximum with increasing photon flux density. This 'fast' component of quenching was identified as energy-dependent quenching qE. It required formation of an appreciable transthylakoid ΔpH and was insignificant when darkened spinach leaves received 1 s pulses of light every 30 s even though zeaxanthin was formed from violaxanthin under these conditions.Another quenching component termed qNs developed in low light without a lag phase. It was not dependent on a transthylakoid pH gradient, decayed exponentially with a long half time of relaxation and was about 20% of total quenching irrespective of light intensity. When darkened leaves were flashed at frequencies higher than 0.004 Hz with 1 s light pulses, this quenching also appeared. Its extent was very considerable, and it did not require formation of zeaxanthin. Relaxation was accelerated by far-red light, and this acceleration was abolished by NaF.We suggest that qNs is the result of a so-called state transition, in which LHC II moves after its phosphorylation from fluorescent PS II to nonfluorescent PS I. This state transition was capable of decreasing in darkened leaves the potential maximum quantum efficiency of electron flow through Photosystem II by about 20%.</description><identifier>ISSN: 0166-8595</identifier><identifier>EISSN: 1573-5079</identifier><identifier>DOI: 10.1007/BF00014888</identifier><identifier>PMID: 24271935</identifier><language>eng</language><publisher>Netherlands</publisher><subject>CHLOROPHYLLE ; CHLOROPHYLLS ; CLOROFILAS ; DARKNESS ; FEUILLE ; FLUORESCENCE ; FLUORESCENCIA ; FOTOSINTESIS ; FOTOSISTEMAS ; HOJAS ; LEAVES ; LIGHT ; LIGHT REGIMES ; LUMIERE ; LUZ ; OBSCURIDAD ; OBSCURITE ; PHOTOSYNTHESE ; PHOTOSYNTHESIS ; PHOTOSYSTEME ; PHOTOSYSTEMS ; REGIME LUMINEUX ; REGIMENES DE LUZ ; SPINACIA OLERACEA</subject><ispartof>Photosynthesis research, 1996-11, Vol.50 (2), p.181-191</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c307t-c1fd0aec1c730752342ccb94553aa36177d77341170dec33cb84ddac9355fe2f3</citedby><cites>FETCH-LOGICAL-c307t-c1fd0aec1c730752342ccb94553aa36177d77341170dec33cb84ddac9355fe2f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24271935$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bukhov, N.G. 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This 'fast' component of quenching was identified as energy-dependent quenching qE. It required formation of an appreciable transthylakoid ΔpH and was insignificant when darkened spinach leaves received 1 s pulses of light every 30 s even though zeaxanthin was formed from violaxanthin under these conditions.Another quenching component termed qNs developed in low light without a lag phase. It was not dependent on a transthylakoid pH gradient, decayed exponentially with a long half time of relaxation and was about 20% of total quenching irrespective of light intensity. When darkened leaves were flashed at frequencies higher than 0.004 Hz with 1 s light pulses, this quenching also appeared. Its extent was very considerable, and it did not require formation of zeaxanthin. Relaxation was accelerated by far-red light, and this acceleration was abolished by NaF.We suggest that qNs is the result of a so-called state transition, in which LHC II moves after its phosphorylation from fluorescent PS II to nonfluorescent PS I. 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(Wuerzburg Univ. (Germany). Julius-von-Sachs-Inst. of Biological Sciences)</creatorcontrib><creatorcontrib>Wiese, C</creatorcontrib><creatorcontrib>Neimanis, S</creatorcontrib><creatorcontrib>Heber, U</creatorcontrib><collection>AGRIS</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Photosynthesis research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bukhov, N.G. (Wuerzburg Univ. (Germany). 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Quenching increased towards a maximum with increasing photon flux density. This 'fast' component of quenching was identified as energy-dependent quenching qE. It required formation of an appreciable transthylakoid ΔpH and was insignificant when darkened spinach leaves received 1 s pulses of light every 30 s even though zeaxanthin was formed from violaxanthin under these conditions.Another quenching component termed qNs developed in low light without a lag phase. It was not dependent on a transthylakoid pH gradient, decayed exponentially with a long half time of relaxation and was about 20% of total quenching irrespective of light intensity. When darkened leaves were flashed at frequencies higher than 0.004 Hz with 1 s light pulses, this quenching also appeared. Its extent was very considerable, and it did not require formation of zeaxanthin. Relaxation was accelerated by far-red light, and this acceleration was abolished by NaF.We suggest that qNs is the result of a so-called state transition, in which LHC II moves after its phosphorylation from fluorescent PS II to nonfluorescent PS I. This state transition was capable of decreasing in darkened leaves the potential maximum quantum efficiency of electron flow through Photosystem II by about 20%.</abstract><cop>Netherlands</cop><pmid>24271935</pmid><doi>10.1007/BF00014888</doi><tpages>11</tpages></addata></record> |
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subjects | CHLOROPHYLLE CHLOROPHYLLS CLOROFILAS DARKNESS FEUILLE FLUORESCENCE FLUORESCENCIA FOTOSINTESIS FOTOSISTEMAS HOJAS LEAVES LIGHT LIGHT REGIMES LUMIERE LUZ OBSCURIDAD OBSCURITE PHOTOSYNTHESE PHOTOSYNTHESIS PHOTOSYSTEME PHOTOSYSTEMS REGIME LUMINEUX REGIMENES DE LUZ SPINACIA OLERACEA |
title | Control of Photosystem II in spinach leaves by continuous light and by light pulses given in the dark |
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