Use of PCR-restriction fragment length polymorphism analysis for identification of yeast species isolated from bovine intramammary infection

This study reports a rapid PCR-based technique using a one-enzyme RFLP for discrimination of yeasts isolated from bovine clinical and subclinical mastitis milk samples. We analyzed a total of 1,486 milk samples collected over 1 yr in south Sardinia and northern Italy, and 142 yeast strains were prel...

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Veröffentlicht in:	Journal of dairy science 2013-12, Vol.96 (12), p.7692-7697
Hauptverfasser:	Fadda, M.E., Pisano, M.B., Scaccabarozzi, L., Mossa, V., Deplano, M., Moroni, P., Liciardi, M., Cosentino, S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence bovine milk Candida Candida - classification Candida - genetics Cattle computer software Cryptococcus - classification Cryptococcus - genetics DNA, Fungal - chemistry DNA, Ribosomal - analysis DNA, Ribosomal - genetics Female Filobasidium France Galactomyces geotrichum genetic variation Geotrichum - classification Geotrichum - genetics Geotrichum candidum Italy mastitis Mastitis, Bovine - microbiology milk Milk - microbiology molecular diagnosis PCR-RFLP Polymerase Chain Reaction Polymorphism, Restriction Fragment Length restriction fragment length polymorphism Rhodotorula - classification Rhodotorula - genetics Rhodotorula glutinis ribosomal DNA Saccharomyces cerevisiae - classification Saccharomyces cerevisiae - genetics Sequence Analysis, DNA species identification Trichosporon Trichosporon - classification Trichosporon - genetics yeasts Yeasts - classification Yeasts - genetics Yeasts - isolation & purification
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