effect of some environmental factors on the production of L-DOPA by alginate-entrapped cells of Mucuna pruriens
In-vitro-grown cells of Mucuna pruriens, immobilized in calcium-alginate gels, were able to transform the precursor L-tyrosine into L-dihydroxyphenylalanine (L-DOPA). After the immobilization in alginate the plant cells released 90% of the produced L-DOPA into the medium; supplementation of the medi...
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| Veröffentlicht in: | Planta 1983-08, Vol.158 (6), p.482-486 |
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| creator | Wichers, H.J Malingre, T.M Huizing, H.J |
| description | In-vitro-grown cells of Mucuna pruriens, immobilized in calcium-alginate gels, were able to transform the precursor L-tyrosine into L-dihydroxyphenylalanine (L-DOPA). After the immobilization in alginate the plant cells released 90% of the produced L-DOPA into the medium; supplementation of the medium with calcium inhibited both the transformation of L-tyrosine into L-DOPA and the release of L-DOPA into the medium. Continuous illumination of the beads had a slight beneficial effect on the synthesis of L-DOPA. A simple production medium for the transformation of L-tyrosine into L-DOPA was designed. This medium contained only sucrose and sodium chloride as osmotic stabilizers, a low concentration of calcium chloride for stabilization of the alginate beads, and L-tyrosine as the precursor. |
| doi_str_mv | 10.1007/bf00397239 |
| format | Article |
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After the immobilization in alginate the plant cells released 90% of the produced L-DOPA into the medium; supplementation of the medium with calcium inhibited both the transformation of L-tyrosine into L-DOPA and the release of L-DOPA into the medium. Continuous illumination of the beads had a slight beneficial effect on the synthesis of L-DOPA. A simple production medium for the transformation of L-tyrosine into L-DOPA was designed. This medium contained only sucrose and sodium chloride as osmotic stabilizers, a low concentration of calcium chloride for stabilization of the alginate beads, and L-tyrosine as the precursor.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/bf00397239</identifier><identifier>PMID: 24264921</identifier><language>eng</language><publisher>Germany: Springer-Verlag</publisher><subject>Alginates ; Calcium ; Chemical suspensions ; Chlorides ; Cultured cells ; dihydroxyphenylalanine ; dopamine ; Immobilized cells ; Mucuna pruriens ; Plant cells ; Sodium ; Stem cells ; Viability</subject><ispartof>Planta, 1983-08, Vol.158 (6), p.482-486</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-bda84be702f6e88cc1195810b6c7c3c7bcc5496a58f76e6894049e91e81099233</citedby><cites>FETCH-LOGICAL-c428t-bda84be702f6e88cc1195810b6c7c3c7bcc5496a58f76e6894049e91e81099233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23376448$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23376448$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>315,781,785,804,27926,27927,58019,58252</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24264921$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wichers, H.J</creatorcontrib><creatorcontrib>Malingre, T.M</creatorcontrib><creatorcontrib>Huizing, H.J</creatorcontrib><title>effect of some environmental factors on the production of L-DOPA by alginate-entrapped cells of Mucuna pruriens</title><title>Planta</title><addtitle>Planta</addtitle><description>In-vitro-grown cells of Mucuna pruriens, immobilized in calcium-alginate gels, were able to transform the precursor L-tyrosine into L-dihydroxyphenylalanine (L-DOPA). 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This medium contained only sucrose and sodium chloride as osmotic stabilizers, a low concentration of calcium chloride for stabilization of the alginate beads, and L-tyrosine as the precursor.</description><subject>Alginates</subject><subject>Calcium</subject><subject>Chemical suspensions</subject><subject>Chlorides</subject><subject>Cultured cells</subject><subject>dihydroxyphenylalanine</subject><subject>dopamine</subject><subject>Immobilized cells</subject><subject>Mucuna pruriens</subject><subject>Plant cells</subject><subject>Sodium</subject><subject>Stem cells</subject><subject>Viability</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><recordid>eNp90ctP3DAQB2CrKioL9NJ7wSeEkALjR2L7SHlLi0CinCPHO4agJF7spBL_PV4tj1tPlv37xhrNEPKLwREDUMeNBxBGcWG-kRmTghccpP5OZvmZF2BEuUm2UnoGyKFSP8gml7yShrMZCeg9upEGT1PokeLwr41h6HEYbUe9dWOIiYaBjk9IlzEsJje2-Zr9vDi7vTuhzSu13WM72BGLXBXtcokL6rDr0krdTG4abC6dYotD2iEb3nYJf76f2-Th4vzv6VUxv728Pj2ZF05yPRbNwmrZoALuK9TaOcZMqRk0lVNOONU4V0pT2VJ7VWGljQRp0DDMxhguxDY5WP-be36ZMI1136ZVU3bAMKWayYppVfKSZ7r_fyqULhWHDA_X0MWQUkRfL2Pb2_haM6hXm6j_XHxsIuPd91-npsfFJ_0YfQa_1-A55Rl_5UKoSkqd87117m2o7WNsU_1wz4EJ4Hm9YEC8AUI9lWk</recordid><startdate>198308</startdate><enddate>198308</enddate><creator>Wichers, H.J</creator><creator>Malingre, T.M</creator><creator>Huizing, H.J</creator><general>Springer-Verlag</general><scope>FBQ</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>198308</creationdate><title>effect of some environmental factors on the production of L-DOPA by alginate-entrapped cells of Mucuna pruriens</title><author>Wichers, H.J ; Malingre, T.M ; Huizing, H.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-bda84be702f6e88cc1195810b6c7c3c7bcc5496a58f76e6894049e91e81099233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Alginates</topic><topic>Calcium</topic><topic>Chemical suspensions</topic><topic>Chlorides</topic><topic>Cultured cells</topic><topic>dihydroxyphenylalanine</topic><topic>dopamine</topic><topic>Immobilized cells</topic><topic>Mucuna pruriens</topic><topic>Plant cells</topic><topic>Sodium</topic><topic>Stem cells</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wichers, H.J</creatorcontrib><creatorcontrib>Malingre, T.M</creatorcontrib><creatorcontrib>Huizing, H.J</creatorcontrib><collection>AGRIS</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wichers, H.J</au><au>Malingre, T.M</au><au>Huizing, H.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>effect of some environmental factors on the production of L-DOPA by alginate-entrapped cells of Mucuna pruriens</atitle><jtitle>Planta</jtitle><addtitle>Planta</addtitle><date>1983-08</date><risdate>1983</risdate><volume>158</volume><issue>6</issue><spage>482</spage><epage>486</epage><pages>482-486</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><abstract>In-vitro-grown cells of Mucuna pruriens, immobilized in calcium-alginate gels, were able to transform the precursor L-tyrosine into L-dihydroxyphenylalanine (L-DOPA). After the immobilization in alginate the plant cells released 90% of the produced L-DOPA into the medium; supplementation of the medium with calcium inhibited both the transformation of L-tyrosine into L-DOPA and the release of L-DOPA into the medium. Continuous illumination of the beads had a slight beneficial effect on the synthesis of L-DOPA. A simple production medium for the transformation of L-tyrosine into L-DOPA was designed. This medium contained only sucrose and sodium chloride as osmotic stabilizers, a low concentration of calcium chloride for stabilization of the alginate beads, and L-tyrosine as the precursor.</abstract><cop>Germany</cop><pub>Springer-Verlag</pub><pmid>24264921</pmid><doi>10.1007/bf00397239</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0935 |
| ispartof | Planta, 1983-08, Vol.158 (6), p.482-486 |
| issn | 0032-0935 1432-2048 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1461875252 |
| source | Springer Journals; JSTOR |
| subjects | Alginates Calcium Chemical suspensions Chlorides Cultured cells dihydroxyphenylalanine dopamine Immobilized cells Mucuna pruriens Plant cells Sodium Stem cells Viability |
| title | effect of some environmental factors on the production of L-DOPA by alginate-entrapped cells of Mucuna pruriens |
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