In Vitro Approaches To Evaluate Toxicity Induced by Organotin Compounds Tributyltin (TBT), Dibutyltin (DBT), and Monobutyltin (MBT) in Neuroblastoma Cells
The toxic effects of the organotin compounds (OTCs) monobutyltin (MBT), dibutyltin (DBT), and tributyltin (TBT) were evaluated in vitro in a neuroblastoma human cell line. Mechanisms of cell death, apoptosis versus necrosis, were studied by using several markers: inhibition of cell viability and pro...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2013-05, Vol.61 (17), p.4195-4203 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Ferreira, Martiña Blanco, Lucía Garrido, Alejandro Vieites, Juan M Cabado, Ana G |
| description | The toxic effects of the organotin compounds (OTCs) monobutyltin (MBT), dibutyltin (DBT), and tributyltin (TBT) were evaluated in vitro in a neuroblastoma human cell line. Mechanisms of cell death, apoptosis versus necrosis, were studied by using several markers: inhibition of cell viability and proliferation, F-actin, and mitochondrial membrane potential changes as well as reactive oxygen species (ROS) production and DNA fragmentation. The most toxic effects were detected with DBT and TBT even at very low concentrations (0.1–1 μM). In contrast, MBT induced lighter cytotoxic changes at the higher doses tested. None of the studied compounds stimulated propidium iodide uptake, although the most toxic chemical, TBT, caused lactate dehydrogenase release at the higher concentrations tested. These findings suggest that in neuroblastoma, OTC-induced cytotoxicity involves different pathways depending on the compound, concentration, and incubation time. A screening method for DBT and TBT quantification based on cell viability loss was developed, allowing a fast detection alternative to complex methodology. |
| doi_str_mv | 10.1021/jf3050186 |
| format | Article |
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Mechanisms of cell death, apoptosis versus necrosis, were studied by using several markers: inhibition of cell viability and proliferation, F-actin, and mitochondrial membrane potential changes as well as reactive oxygen species (ROS) production and DNA fragmentation. The most toxic effects were detected with DBT and TBT even at very low concentrations (0.1–1 μM). In contrast, MBT induced lighter cytotoxic changes at the higher doses tested. None of the studied compounds stimulated propidium iodide uptake, although the most toxic chemical, TBT, caused lactate dehydrogenase release at the higher concentrations tested. These findings suggest that in neuroblastoma, OTC-induced cytotoxicity involves different pathways depending on the compound, concentration, and incubation time. A screening method for DBT and TBT quantification based on cell viability loss was developed, allowing a fast detection alternative to complex methodology.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf3050186</identifier><identifier>PMID: 23534342</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>actin ; apoptosis ; Biological and medical sciences ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; cell viability ; cytotoxicity ; DNA fragmentation ; DNA Fragmentation - drug effects ; Food industries ; Fundamental and applied biological sciences. Psychology ; Humans ; L-Lactate Dehydrogenase - metabolism ; lactate dehydrogenase ; membrane potential ; Membrane Potential, Mitochondrial - drug effects ; necrosis ; Neuroblastoma - metabolism ; Neurotoxins - toxicity ; organotin compounds ; Organotin Compounds - toxicity ; propidium ; Reactive Oxygen Species ; Toxicity Tests - methods ; Trialkyltin Compounds - toxicity</subject><ispartof>Journal of agricultural and food chemistry, 2013-05, Vol.61 (17), p.4195-4203</ispartof><rights>Copyright © 2013 American Chemical Society</rights><rights>2014 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a435t-9ff54e8a4530b3d8216dc5650fcd1482003ad43615efcf500476270ef6a9fbf33</citedby><cites>FETCH-LOGICAL-a435t-9ff54e8a4530b3d8216dc5650fcd1482003ad43615efcf500476270ef6a9fbf33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf3050186$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf3050186$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27322193$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23534342$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ferreira, Martiña</creatorcontrib><creatorcontrib>Blanco, Lucía</creatorcontrib><creatorcontrib>Garrido, Alejandro</creatorcontrib><creatorcontrib>Vieites, Juan M</creatorcontrib><creatorcontrib>Cabado, Ana G</creatorcontrib><title>In Vitro Approaches To Evaluate Toxicity Induced by Organotin Compounds Tributyltin (TBT), Dibutyltin (DBT), and Monobutyltin (MBT) in Neuroblastoma Cells</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>The toxic effects of the organotin compounds (OTCs) monobutyltin (MBT), dibutyltin (DBT), and tributyltin (TBT) were evaluated in vitro in a neuroblastoma human cell line. Mechanisms of cell death, apoptosis versus necrosis, were studied by using several markers: inhibition of cell viability and proliferation, F-actin, and mitochondrial membrane potential changes as well as reactive oxygen species (ROS) production and DNA fragmentation. The most toxic effects were detected with DBT and TBT even at very low concentrations (0.1–1 μM). In contrast, MBT induced lighter cytotoxic changes at the higher doses tested. None of the studied compounds stimulated propidium iodide uptake, although the most toxic chemical, TBT, caused lactate dehydrogenase release at the higher concentrations tested. These findings suggest that in neuroblastoma, OTC-induced cytotoxicity involves different pathways depending on the compound, concentration, and incubation time. A screening method for DBT and TBT quantification based on cell viability loss was developed, allowing a fast detection alternative to complex methodology.</description><subject>actin</subject><subject>apoptosis</subject><subject>Biological and medical sciences</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>cell viability</subject><subject>cytotoxicity</subject><subject>DNA fragmentation</subject><subject>DNA Fragmentation - drug effects</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>lactate dehydrogenase</subject><subject>membrane potential</subject><subject>Membrane Potential, Mitochondrial - drug effects</subject><subject>necrosis</subject><subject>Neuroblastoma - metabolism</subject><subject>Neurotoxins - toxicity</subject><subject>organotin compounds</subject><subject>Organotin Compounds - toxicity</subject><subject>propidium</subject><subject>Reactive Oxygen Species</subject><subject>Toxicity Tests - methods</subject><subject>Trialkyltin Compounds - toxicity</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkc1uEzEUhS1ERUNhwQuAN0it1IHrv_lZlrRApJYuSNmO7njsMtGMndoziLwKT4tDQsOCla-OPx8dn0vIKwbvGHD2fmUFKGBl_oTMmOKQKcbKp2QG6TIrVc6OyfMYVwBQqgKekWMulJBC8hn5tXD0WzcGTy_W6-BRfzeRLj29-oH9hKNJ889Od-OGLlw7adPSZkNvwz06P3aOzv2w9pNr05vQNdO46bfq6fLD8uycXv6jXP5R0LX0xjt_0G-STtPwxUzBNz3G0Q9I56bv4wtyZLGP5uX-PCF3H6-W88_Z9e2nxfziOkMp1JhV1ippSpRKQCPakrO81SpXYHXLZMkBBLZS5EwZq60CkEXOCzA2x8o2VogTcrrzTf9_mEwc66GLOiVAZ_wUayZzVhap1zyhZztUBx9jMLZeh27AsKkZ1NtV1I-rSOzrve3UDKZ9JP92n4C3ewCjxt4GdLqLB64QnLNqm-_NjrPoa7wPibn7yoFJAMarqqgOTqhjvfJTcKmv_0T6DUF7pLI</recordid><startdate>20130501</startdate><enddate>20130501</enddate><creator>Ferreira, Martiña</creator><creator>Blanco, Lucía</creator><creator>Garrido, Alejandro</creator><creator>Vieites, Juan M</creator><creator>Cabado, Ana G</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130501</creationdate><title>In Vitro Approaches To Evaluate Toxicity Induced by Organotin Compounds Tributyltin (TBT), Dibutyltin (DBT), and Monobutyltin (MBT) in Neuroblastoma Cells</title><author>Ferreira, Martiña ; Blanco, Lucía ; Garrido, Alejandro ; Vieites, Juan M ; Cabado, Ana G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a435t-9ff54e8a4530b3d8216dc5650fcd1482003ad43615efcf500476270ef6a9fbf33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>actin</topic><topic>apoptosis</topic><topic>Biological and medical sciences</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>cell viability</topic><topic>cytotoxicity</topic><topic>DNA fragmentation</topic><topic>DNA Fragmentation - drug effects</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>lactate dehydrogenase</topic><topic>membrane potential</topic><topic>Membrane Potential, Mitochondrial - drug effects</topic><topic>necrosis</topic><topic>Neuroblastoma - metabolism</topic><topic>Neurotoxins - toxicity</topic><topic>organotin compounds</topic><topic>Organotin Compounds - toxicity</topic><topic>propidium</topic><topic>Reactive Oxygen Species</topic><topic>Toxicity Tests - methods</topic><topic>Trialkyltin Compounds - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ferreira, Martiña</creatorcontrib><creatorcontrib>Blanco, Lucía</creatorcontrib><creatorcontrib>Garrido, Alejandro</creatorcontrib><creatorcontrib>Vieites, Juan M</creatorcontrib><creatorcontrib>Cabado, Ana G</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ferreira, Martiña</au><au>Blanco, Lucía</au><au>Garrido, Alejandro</au><au>Vieites, Juan M</au><au>Cabado, Ana G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Vitro Approaches To Evaluate Toxicity Induced by Organotin Compounds Tributyltin (TBT), Dibutyltin (DBT), and Monobutyltin (MBT) in Neuroblastoma Cells</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2013-05-01</date><risdate>2013</risdate><volume>61</volume><issue>17</issue><spage>4195</spage><epage>4203</epage><pages>4195-4203</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>The toxic effects of the organotin compounds (OTCs) monobutyltin (MBT), dibutyltin (DBT), and tributyltin (TBT) were evaluated in vitro in a neuroblastoma human cell line. Mechanisms of cell death, apoptosis versus necrosis, were studied by using several markers: inhibition of cell viability and proliferation, F-actin, and mitochondrial membrane potential changes as well as reactive oxygen species (ROS) production and DNA fragmentation. The most toxic effects were detected with DBT and TBT even at very low concentrations (0.1–1 μM). In contrast, MBT induced lighter cytotoxic changes at the higher doses tested. None of the studied compounds stimulated propidium iodide uptake, although the most toxic chemical, TBT, caused lactate dehydrogenase release at the higher concentrations tested. These findings suggest that in neuroblastoma, OTC-induced cytotoxicity involves different pathways depending on the compound, concentration, and incubation time. A screening method for DBT and TBT quantification based on cell viability loss was developed, allowing a fast detection alternative to complex methodology.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>23534342</pmid><doi>10.1021/jf3050186</doi><tpages>9</tpages></addata></record> |
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| subjects | actin apoptosis Biological and medical sciences Cell Line, Tumor Cell Proliferation - drug effects Cell Survival - drug effects cell viability cytotoxicity DNA fragmentation DNA Fragmentation - drug effects Food industries Fundamental and applied biological sciences. Psychology Humans L-Lactate Dehydrogenase - metabolism lactate dehydrogenase membrane potential Membrane Potential, Mitochondrial - drug effects necrosis Neuroblastoma - metabolism Neurotoxins - toxicity organotin compounds Organotin Compounds - toxicity propidium Reactive Oxygen Species Toxicity Tests - methods Trialkyltin Compounds - toxicity |
| title | In Vitro Approaches To Evaluate Toxicity Induced by Organotin Compounds Tributyltin (TBT), Dibutyltin (DBT), and Monobutyltin (MBT) in Neuroblastoma Cells |
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