Rapid Determination of Amino Acids in Fruits of Ziziphus jujuba by Hydrophilic Interaction Ultra-High-Performance Liquid Chromatography Coupled with Triple-Quadrupole Mass Spectrometry
In this study, a sensitive and rapid method for the simultaneous determination of free amino acids without derivatization using hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry (HILIC–MS/MS) was developed. The method was performed on an ultra-high-performance liqui...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2013-03, Vol.61 (11), p.2709-2719 |
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| container_issue | 11 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Guo, Sheng Duan, Jin-ao Qian, Dawei Tang, Yuping Qian, Yefei Wu, Dawei Su, Shulan Shang, Erxin |
| description | In this study, a sensitive and rapid method for the simultaneous determination of free amino acids without derivatization using hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry (HILIC–MS/MS) was developed. The method was performed on an ultra-high-performance liquid chromatography (UHPLC) separation system coupled with a triple-quadrupole mass spectrometry (TQ-MS) instrument. Sufficient separation of 23 underivatized amino acids was achieved on an Acquity BEH Amide column (2.1 mm × 100 mm, 1.7 μm) in a single run of 12 min. Then the method was applied for the analysis of the free amino acids in 46 batches of Ziziphus jujuba fruits which comprised 39 cultivars from 26 cultivation regions. Multivariate statistical analysis was also used to investigate the differences in free amino acid profiles among the samples. This study showed that HILIC–UHPLC–TQ-MS is an effective technique to analyze underivatized amino acids in the food samples. |
| doi_str_mv | 10.1021/jf305497r |
| format | Article |
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The method was performed on an ultra-high-performance liquid chromatography (UHPLC) separation system coupled with a triple-quadrupole mass spectrometry (TQ-MS) instrument. Sufficient separation of 23 underivatized amino acids was achieved on an Acquity BEH Amide column (2.1 mm × 100 mm, 1.7 μm) in a single run of 12 min. Then the method was applied for the analysis of the free amino acids in 46 batches of Ziziphus jujuba fruits which comprised 39 cultivars from 26 cultivation regions. Multivariate statistical analysis was also used to investigate the differences in free amino acid profiles among the samples. This study showed that HILIC–UHPLC–TQ-MS is an effective technique to analyze underivatized amino acids in the food samples.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf305497r</identifier><identifier>PMID: 23413770</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Amino Acids - chemistry ; Biological and medical sciences ; Chromatography, High Pressure Liquid - methods ; Food industries ; Fruit - chemistry ; Fruit and vegetable industries ; Fundamental and applied biological sciences. Psychology ; Mass Spectrometry - methods ; Ziziphus - chemistry</subject><ispartof>Journal of agricultural and food chemistry, 2013-03, Vol.61 (11), p.2709-2719</ispartof><rights>Copyright © 2013 American Chemical Society</rights><rights>2014 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf305497r$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf305497r$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27247232$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23413770$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Sheng</creatorcontrib><creatorcontrib>Duan, Jin-ao</creatorcontrib><creatorcontrib>Qian, Dawei</creatorcontrib><creatorcontrib>Tang, Yuping</creatorcontrib><creatorcontrib>Qian, Yefei</creatorcontrib><creatorcontrib>Wu, Dawei</creatorcontrib><creatorcontrib>Su, Shulan</creatorcontrib><creatorcontrib>Shang, Erxin</creatorcontrib><title>Rapid Determination of Amino Acids in Fruits of Ziziphus jujuba by Hydrophilic Interaction Ultra-High-Performance Liquid Chromatography Coupled with Triple-Quadrupole Mass Spectrometry</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>In this study, a sensitive and rapid method for the simultaneous determination of free amino acids without derivatization using hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry (HILIC–MS/MS) was developed. The method was performed on an ultra-high-performance liquid chromatography (UHPLC) separation system coupled with a triple-quadrupole mass spectrometry (TQ-MS) instrument. Sufficient separation of 23 underivatized amino acids was achieved on an Acquity BEH Amide column (2.1 mm × 100 mm, 1.7 μm) in a single run of 12 min. Then the method was applied for the analysis of the free amino acids in 46 batches of Ziziphus jujuba fruits which comprised 39 cultivars from 26 cultivation regions. Multivariate statistical analysis was also used to investigate the differences in free amino acid profiles among the samples. This study showed that HILIC–UHPLC–TQ-MS is an effective technique to analyze underivatized amino acids in the food samples.</description><subject>Amino Acids - chemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Food industries</subject><subject>Fruit - chemistry</subject><subject>Fruit and vegetable industries</subject><subject>Fundamental and applied biological sciences. 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Agric. Food Chem</addtitle><date>2013-03-20</date><risdate>2013</risdate><volume>61</volume><issue>11</issue><spage>2709</spage><epage>2719</epage><pages>2709-2719</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>In this study, a sensitive and rapid method for the simultaneous determination of free amino acids without derivatization using hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry (HILIC–MS/MS) was developed. The method was performed on an ultra-high-performance liquid chromatography (UHPLC) separation system coupled with a triple-quadrupole mass spectrometry (TQ-MS) instrument. Sufficient separation of 23 underivatized amino acids was achieved on an Acquity BEH Amide column (2.1 mm × 100 mm, 1.7 μm) in a single run of 12 min. Then the method was applied for the analysis of the free amino acids in 46 batches of Ziziphus jujuba fruits which comprised 39 cultivars from 26 cultivation regions. 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| ispartof | Journal of agricultural and food chemistry, 2013-03, Vol.61 (11), p.2709-2719 |
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| language | eng |
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| source | ACS Publications; MEDLINE |
| subjects | Amino Acids - chemistry Biological and medical sciences Chromatography, High Pressure Liquid - methods Food industries Fruit - chemistry Fruit and vegetable industries Fundamental and applied biological sciences. Psychology Mass Spectrometry - methods Ziziphus - chemistry |
| title | Rapid Determination of Amino Acids in Fruits of Ziziphus jujuba by Hydrophilic Interaction Ultra-High-Performance Liquid Chromatography Coupled with Triple-Quadrupole Mass Spectrometry |
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