Bovine Milk Fat Globule Membrane Proteins Are Affected By Centrifugal Washing Processes
The impact of washing on the release of proteins from the milk fat globule membrane (MFGM) was examined by applying washing procedures with different degrees of stringency to milk fat globule (MFG) surfaces in simulated milk ultrafiltrate buffer solution. Three washing methods, M1 (3000g, 5 min, thr...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2013-09, Vol.61 (35), p.8403-8411 |
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| creator | Zheng, Haotian Jiménez-Flores, Rafael Everett, David W |
| description | The impact of washing on the release of proteins from the milk fat globule membrane (MFGM) was examined by applying washing procedures with different degrees of stringency to milk fat globule (MFG) surfaces in simulated milk ultrafiltrate buffer solution. Three washing methods, M1 (3000g, 5 min, three washes), M2 (3750g, 15 min, one wash), and M3 (15000g, 20 min, three washes) were chosen. MFG ζ-potential increased after M3 washing (P < 0.05), suggesting surface damage. For M1, in which the native MFG surface was least damaged, cluster of differentiation 36 (CD 36) and periodic acid schiff 6/7 proteins were more strongly bound to the MFGM compared with other major membrane proteins. For M3, CD 36 together with fatty acid-binding protein was more strongly bound to the MFGM. Washing by centrifugation and redispersal of the fat globules damaged the MFGM, with release into the aqueous phase of some membrane-associated proteins. The current results show the impact of washing processes on retention of functional MFGM proteins. |
| doi_str_mv | 10.1021/jf402591f |
| format | Article |
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Three washing methods, M1 (3000g, 5 min, three washes), M2 (3750g, 15 min, one wash), and M3 (15000g, 20 min, three washes) were chosen. MFG ζ-potential increased after M3 washing (P < 0.05), suggesting surface damage. For M1, in which the native MFG surface was least damaged, cluster of differentiation 36 (CD 36) and periodic acid schiff 6/7 proteins were more strongly bound to the MFGM compared with other major membrane proteins. For M3, CD 36 together with fatty acid-binding protein was more strongly bound to the MFGM. Washing by centrifugation and redispersal of the fat globules damaged the MFGM, with release into the aqueous phase of some membrane-associated proteins. The current results show the impact of washing processes on retention of functional MFGM proteins.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf402591f</identifier><identifier>PMID: 23931678</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animals ; Biological and medical sciences ; Cattle ; Centrifugation ; fat globules ; Fats - analysis ; fatty acid-binding proteins ; Food industries ; Fundamental and applied biological sciences. Psychology ; Glycolipids - chemistry ; Glycoproteins - chemistry ; Glycoproteins - ultrastructure ; Humans ; membrane proteins ; Membrane Proteins - chemistry ; Membrane Proteins - ultrastructure ; Microscopy, Confocal ; milk ; Milk - chemistry ; Milk and cheese industries. Ice creams ; milk fat ; Milk Proteins - analysis ; washing</subject><ispartof>Journal of agricultural and food chemistry, 2013-09, Vol.61 (35), p.8403-8411</ispartof><rights>Copyright © 2013 American Chemical Society</rights><rights>2014 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a369t-a9c4d294ea05587b75eeca5f29b234b5c482869545ed564c413fb3389b6d99003</citedby><cites>FETCH-LOGICAL-a369t-a9c4d294ea05587b75eeca5f29b234b5c482869545ed564c413fb3389b6d99003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf402591f$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf402591f$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27723035$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23931678$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zheng, Haotian</creatorcontrib><creatorcontrib>Jiménez-Flores, Rafael</creatorcontrib><creatorcontrib>Everett, David W</creatorcontrib><title>Bovine Milk Fat Globule Membrane Proteins Are Affected By Centrifugal Washing Processes</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>The impact of washing on the release of proteins from the milk fat globule membrane (MFGM) was examined by applying washing procedures with different degrees of stringency to milk fat globule (MFG) surfaces in simulated milk ultrafiltrate buffer solution. Three washing methods, M1 (3000g, 5 min, three washes), M2 (3750g, 15 min, one wash), and M3 (15000g, 20 min, three washes) were chosen. MFG ζ-potential increased after M3 washing (P < 0.05), suggesting surface damage. For M1, in which the native MFG surface was least damaged, cluster of differentiation 36 (CD 36) and periodic acid schiff 6/7 proteins were more strongly bound to the MFGM compared with other major membrane proteins. For M3, CD 36 together with fatty acid-binding protein was more strongly bound to the MFGM. Washing by centrifugation and redispersal of the fat globules damaged the MFGM, with release into the aqueous phase of some membrane-associated proteins. The current results show the impact of washing processes on retention of functional MFGM proteins.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Centrifugation</subject><subject>fat globules</subject><subject>Fats - analysis</subject><subject>fatty acid-binding proteins</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycolipids - chemistry</subject><subject>Glycoproteins - chemistry</subject><subject>Glycoproteins - ultrastructure</subject><subject>Humans</subject><subject>membrane proteins</subject><subject>Membrane Proteins - chemistry</subject><subject>Membrane Proteins - ultrastructure</subject><subject>Microscopy, Confocal</subject><subject>milk</subject><subject>Milk - chemistry</subject><subject>Milk and cheese industries. Ice creams</subject><subject>milk fat</subject><subject>Milk Proteins - analysis</subject><subject>washing</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0MtKxDAUBuAgio6XhS-g3Qi6qObaJsuZwRuMKKi4LGl6MnbsZUxawbc3ZcZx4ypwzsefw4_QMcGXBFNytbAcU6GI3UIjIiiOBSFyG41wWMZSJGQP7Xu_wBhLkeJdtEeZYiRJ5Qi9TdqvsoHooaw-ohvdRbdVm_dVGECdOx02T67toGx8NHYQja0F00ERTb6jKTSdK20_11X0pv172cwHbMB78Idox-rKw9H6PUCvN9cv07t49nh7Px3PYs0S1cVaGV5QxUFjIWSapwLAaGGpyinjuTBcUpkowQUUIuGGE2ZzxqTKk0IpjNkBOl_lLl372YPvsrr0BqoqnN72PiM8ITIRlA30YkWNa713YLOlK2vtvjOCs6HHbNNjsCfr2D6vodjI3-ICOFsD7Y2ubKjKlP7PpenwpQjudOWsbjM9d8G8PlNMOMaEUcLTvyRtfLZoe9eEvv456Qf9RI2p</recordid><startdate>20130904</startdate><enddate>20130904</enddate><creator>Zheng, Haotian</creator><creator>Jiménez-Flores, Rafael</creator><creator>Everett, David W</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130904</creationdate><title>Bovine Milk Fat Globule Membrane Proteins Are Affected By Centrifugal Washing Processes</title><author>Zheng, Haotian ; Jiménez-Flores, Rafael ; Everett, David W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a369t-a9c4d294ea05587b75eeca5f29b234b5c482869545ed564c413fb3389b6d99003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Centrifugation</topic><topic>fat globules</topic><topic>Fats - analysis</topic><topic>fatty acid-binding proteins</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycolipids - chemistry</topic><topic>Glycoproteins - chemistry</topic><topic>Glycoproteins - ultrastructure</topic><topic>Humans</topic><topic>membrane proteins</topic><topic>Membrane Proteins - chemistry</topic><topic>Membrane Proteins - ultrastructure</topic><topic>Microscopy, Confocal</topic><topic>milk</topic><topic>Milk - chemistry</topic><topic>Milk and cheese industries. Ice creams</topic><topic>milk fat</topic><topic>Milk Proteins - analysis</topic><topic>washing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zheng, Haotian</creatorcontrib><creatorcontrib>Jiménez-Flores, Rafael</creatorcontrib><creatorcontrib>Everett, David W</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zheng, Haotian</au><au>Jiménez-Flores, Rafael</au><au>Everett, David W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bovine Milk Fat Globule Membrane Proteins Are Affected By Centrifugal Washing Processes</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2013-09-04</date><risdate>2013</risdate><volume>61</volume><issue>35</issue><spage>8403</spage><epage>8411</epage><pages>8403-8411</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>The impact of washing on the release of proteins from the milk fat globule membrane (MFGM) was examined by applying washing procedures with different degrees of stringency to milk fat globule (MFG) surfaces in simulated milk ultrafiltrate buffer solution. Three washing methods, M1 (3000g, 5 min, three washes), M2 (3750g, 15 min, one wash), and M3 (15000g, 20 min, three washes) were chosen. MFG ζ-potential increased after M3 washing (P < 0.05), suggesting surface damage. For M1, in which the native MFG surface was least damaged, cluster of differentiation 36 (CD 36) and periodic acid schiff 6/7 proteins were more strongly bound to the MFGM compared with other major membrane proteins. For M3, CD 36 together with fatty acid-binding protein was more strongly bound to the MFGM. Washing by centrifugation and redispersal of the fat globules damaged the MFGM, with release into the aqueous phase of some membrane-associated proteins. The current results show the impact of washing processes on retention of functional MFGM proteins.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>23931678</pmid><doi>10.1021/jf402591f</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Cattle Centrifugation fat globules Fats - analysis fatty acid-binding proteins Food industries Fundamental and applied biological sciences. Psychology Glycolipids - chemistry Glycoproteins - chemistry Glycoproteins - ultrastructure Humans membrane proteins Membrane Proteins - chemistry Membrane Proteins - ultrastructure Microscopy, Confocal milk Milk - chemistry Milk and cheese industries. Ice creams milk fat Milk Proteins - analysis washing |
| title | Bovine Milk Fat Globule Membrane Proteins Are Affected By Centrifugal Washing Processes |
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