Separation of peptide mixtures by reversed-phase gradient elution. Use of flow rate changes for controlling band spacing and improving resolution

Separation of peptide mixtures by reversed-phase gradient elution. Use of flow rate changes for controlling band spacing and improving resolution

A general model has recently been proposed for the separation of peptides and proteins using reversed-phase gradient elution liquid chromatography. One application of this model suggests that flow rate, gradient time, or column configuration can be varied for band spacing control in the separation o...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Analytical chemistry (Washington) 1986-02, Vol.58 (2), p.280-285
Hauptverfasser: Glajch, J. L, Quarry, M. A, Vasta, J. F, Snyder, L. R
Format: Artikel
Sprache:eng
Schlagworte:
Applied sciences
chromatography
Exact sciences and technology
Other techniques and industries
proteins
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 285
container_issue 2
container_start_page 280
container_title Analytical chemistry (Washington)
container_volume 58
creator Glajch, J. L
Quarry, M. A
Vasta, J. F
Snyder, L. R
description A general model has recently been proposed for the separation of peptides and proteins using reversed-phase gradient elution liquid chromatography. One application of this model suggests that flow rate, gradient time, or column configuration can be varied for band spacing control in the separation of enzymatic digests of proteins. Here a systematic procedure is described that uses repeated separations with different flow rates to maximize the separation of individual peaks within the chromatogram. From these initial separations it is possible to choose and optimum flow rate for the separation of a given sample.
doi_str_mv 10.1021/ac00293a004
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_14616398</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>14616398</sourcerecordid><originalsourceid>FETCH-LOGICAL-a397t-848ef55f16780adf40c51835a03cc758a5bb5708579b462ae0e65949078cbad73</originalsourceid><addsrcrecordid>eNptkM1u1TAQhS0EEpfCihfwAsECpYyTOHaW6PIrVaLVbdlaE2dy65IbBzsp7WP0jesoVcWiq5mxv3NmdBh7K-BYQC4-oQXI6wIBymdsI2QOWaV1_pxtAKDIcgXwkr2K8QpACBDVht3taMSAk_MD9x0faZxcS_zgbqY5UOTNLQ90TSFSm42XGInvA7aOholTPy-yY36RXpO26_0_nqyI20sc9knc-cCtH6bg-94Ne97g0PI4ol2GpXeHMfjrZUq7_Or3mr3osI_05qEesYtvX8-3P7KTX99_bj-fZFjUasp0qamTshOV0oBtV4KVQhcSobBWSY2yaaQCLVXdlFWOBFTJuqxBadtgq4oj9n71TSf8nSlO5uCipb7HgfwcjSgrURW1TuDHFbTBxxioM2NwBwy3RoBZYjf_xZ7odw-2GC32XcDBuvgo0ZBrlS_bsxVzcaKbx28Mf0ylCiXN-enOgPxSbU9_n5ld4j-sPNporvwchpTNkwfcA_Mkn-Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14616398</pqid></control><display><type>article</type><title>Separation of peptide mixtures by reversed-phase gradient elution. Use of flow rate changes for controlling band spacing and improving resolution</title><source>American Chemical Society Journals</source><creator>Glajch, J. L ; Quarry, M. A ; Vasta, J. F ; Snyder, L. R</creator><creatorcontrib>Glajch, J. L ; Quarry, M. A ; Vasta, J. F ; Snyder, L. R</creatorcontrib><description>A general model has recently been proposed for the separation of peptides and proteins using reversed-phase gradient elution liquid chromatography. One application of this model suggests that flow rate, gradient time, or column configuration can be varied for band spacing control in the separation of enzymatic digests of proteins. Here a systematic procedure is described that uses repeated separations with different flow rates to maximize the separation of individual peaks within the chromatogram. From these initial separations it is possible to choose and optimum flow rate for the separation of a given sample.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac00293a004</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Applied sciences ; chromatography ; Exact sciences and technology ; Other techniques and industries ; proteins</subject><ispartof>Analytical chemistry (Washington), 1986-02, Vol.58 (2), p.280-285</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a397t-848ef55f16780adf40c51835a03cc758a5bb5708579b462ae0e65949078cbad73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac00293a004$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac00293a004$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=8028727$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Glajch, J. L</creatorcontrib><creatorcontrib>Quarry, M. A</creatorcontrib><creatorcontrib>Vasta, J. F</creatorcontrib><creatorcontrib>Snyder, L. R</creatorcontrib><title>Separation of peptide mixtures by reversed-phase gradient elution. Use of flow rate changes for controlling band spacing and improving resolution</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>A general model has recently been proposed for the separation of peptides and proteins using reversed-phase gradient elution liquid chromatography. One application of this model suggests that flow rate, gradient time, or column configuration can be varied for band spacing control in the separation of enzymatic digests of proteins. Here a systematic procedure is described that uses repeated separations with different flow rates to maximize the separation of individual peaks within the chromatogram. From these initial separations it is possible to choose and optimum flow rate for the separation of a given sample.</description><subject>Applied sciences</subject><subject>chromatography</subject><subject>Exact sciences and technology</subject><subject>Other techniques and industries</subject><subject>proteins</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><recordid>eNptkM1u1TAQhS0EEpfCihfwAsECpYyTOHaW6PIrVaLVbdlaE2dy65IbBzsp7WP0jesoVcWiq5mxv3NmdBh7K-BYQC4-oQXI6wIBymdsI2QOWaV1_pxtAKDIcgXwkr2K8QpACBDVht3taMSAk_MD9x0faZxcS_zgbqY5UOTNLQ90TSFSm42XGInvA7aOholTPy-yY36RXpO26_0_nqyI20sc9knc-cCtH6bg-94Ne97g0PI4ol2GpXeHMfjrZUq7_Or3mr3osI_05qEesYtvX8-3P7KTX99_bj-fZFjUasp0qamTshOV0oBtV4KVQhcSobBWSY2yaaQCLVXdlFWOBFTJuqxBadtgq4oj9n71TSf8nSlO5uCipb7HgfwcjSgrURW1TuDHFbTBxxioM2NwBwy3RoBZYjf_xZ7odw-2GC32XcDBuvgo0ZBrlS_bsxVzcaKbx28Mf0ylCiXN-enOgPxSbU9_n5ld4j-sPNporvwchpTNkwfcA_Mkn-Q</recordid><startdate>19860201</startdate><enddate>19860201</enddate><creator>Glajch, J. L</creator><creator>Quarry, M. A</creator><creator>Vasta, J. F</creator><creator>Snyder, L. R</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>19860201</creationdate><title>Separation of peptide mixtures by reversed-phase gradient elution. Use of flow rate changes for controlling band spacing and improving resolution</title><author>Glajch, J. L ; Quarry, M. A ; Vasta, J. F ; Snyder, L. R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a397t-848ef55f16780adf40c51835a03cc758a5bb5708579b462ae0e65949078cbad73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Applied sciences</topic><topic>chromatography</topic><topic>Exact sciences and technology</topic><topic>Other techniques and industries</topic><topic>proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Glajch, J. L</creatorcontrib><creatorcontrib>Quarry, M. A</creatorcontrib><creatorcontrib>Vasta, J. F</creatorcontrib><creatorcontrib>Snyder, L. R</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Glajch, J. L</au><au>Quarry, M. A</au><au>Vasta, J. F</au><au>Snyder, L. R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Separation of peptide mixtures by reversed-phase gradient elution. Use of flow rate changes for controlling band spacing and improving resolution</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>1986-02-01</date><risdate>1986</risdate><volume>58</volume><issue>2</issue><spage>280</spage><epage>285</epage><pages>280-285</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>A general model has recently been proposed for the separation of peptides and proteins using reversed-phase gradient elution liquid chromatography. One application of this model suggests that flow rate, gradient time, or column configuration can be varied for band spacing control in the separation of enzymatic digests of proteins. Here a systematic procedure is described that uses repeated separations with different flow rates to maximize the separation of individual peaks within the chromatogram. From these initial separations it is possible to choose and optimum flow rate for the separation of a given sample.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><doi>10.1021/ac00293a004</doi><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0003-2700
ispartof Analytical chemistry (Washington), 1986-02, Vol.58 (2), p.280-285
issn 0003-2700
1520-6882
language eng
recordid cdi_proquest_miscellaneous_14616398
source American Chemical Society Journals
subjects Applied sciences
chromatography
Exact sciences and technology
Other techniques and industries
proteins
title Separation of peptide mixtures by reversed-phase gradient elution. Use of flow rate changes for controlling band spacing and improving resolution
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T04%3A45%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Separation%20of%20peptide%20mixtures%20by%20reversed-phase%20gradient%20elution.%20Use%20of%20flow%20rate%20changes%20for%20controlling%20band%20spacing%20and%20improving%20resolution&rft.jtitle=Analytical%20chemistry%20(Washington)&rft.au=Glajch,%20J.%20L&rft.date=1986-02-01&rft.volume=58&rft.issue=2&rft.spage=280&rft.epage=285&rft.pages=280-285&rft.issn=0003-2700&rft.eissn=1520-6882&rft.coden=ANCHAM&rft_id=info:doi/10.1021/ac00293a004&rft_dat=%3Cproquest_cross%3E14616398%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14616398&rft_id=info:pmid/&rfr_iscdi=true