Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana)

Opening of apical and axillary buds of mature Douglas-fir and sugar pine trees was obtained on a newly formulated basal medium (DCR) without growth regulators. Elongation of buds was observed on 1/2 strength DCR with 0.3% activated charcoal (DCR-1). In sugar pine, multiple shoots were obtained when...

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Veröffentlicht in:Plant cell reports 1985-08, Vol.4 (4), p.177-179
Hauptverfasser: Gupta, P.K, Durzan, D.J
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description Opening of apical and axillary buds of mature Douglas-fir and sugar pine trees was obtained on a newly formulated basal medium (DCR) without growth regulators. Elongation of buds was observed on 1/2 strength DCR with 0.3% activated charcoal (DCR-1). In sugar pine, multiple shoots were obtained when explants on DCR with 0.5 mg/1 BAP for 5-6 weeks were transferred to DCR-1 medium. On subculture, axillary buds again developed when shoots were cultured on DCR with 0.2 mg/1 BAP for Douglas-fir and 0.5 mg/1 BAP for sugar pine. These buds were again elongated on DCR-1 medium. By subculturing 7-10 shoots of Douglas-fir and 2-3 shoots of sugar pine, over 100 shoots can be obtained in a year.
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source SpringerNature Journals
subjects Artificial regeneration. Forest nurseries. Planting
Biological and medical sciences
Biotechnology
Eukaryotic cell cultures
Forestry
Fundamental and applied biological sciences. Psychology
In vitro propagation: entire plant regeneration from tissues and cell cultures
Methods. Procedures. Technologies
Pinus lambertiana
Plant cells and fungal cells
Plant physiology and development
plant propagation
Pseudotsuga menziesii
shoots
Tissue cultures, protoplasts
Vegetative propagation. Micropropagation
title Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana)
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