Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana)
Opening of apical and axillary buds of mature Douglas-fir and sugar pine trees was obtained on a newly formulated basal medium (DCR) without growth regulators. Elongation of buds was observed on 1/2 strength DCR with 0.3% activated charcoal (DCR-1). In sugar pine, multiple shoots were obtained when...
Gespeichert in:
Veröffentlicht in: | Plant cell reports 1985-08, Vol.4 (4), p.177-179 |
---|---|
Hauptverfasser: | , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 179 |
---|---|
container_issue | 4 |
container_start_page | 177 |
container_title | Plant cell reports |
container_volume | 4 |
creator | Gupta, P.K Durzan, D.J |
description | Opening of apical and axillary buds of mature Douglas-fir and sugar pine trees was obtained on a newly formulated basal medium (DCR) without growth regulators. Elongation of buds was observed on 1/2 strength DCR with 0.3% activated charcoal (DCR-1). In sugar pine, multiple shoots were obtained when explants on DCR with 0.5 mg/1 BAP for 5-6 weeks were transferred to DCR-1 medium. On subculture, axillary buds again developed when shoots were cultured on DCR with 0.2 mg/1 BAP for Douglas-fir and 0.5 mg/1 BAP for sugar pine. These buds were again elongated on DCR-1 medium. By subculturing 7-10 shoots of Douglas-fir and 2-3 shoots of sugar pine, over 100 shoots can be obtained in a year. |
doi_str_mv | 10.1007/bf00269282 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1461344096</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1461344096</sourcerecordid><originalsourceid>FETCH-LOGICAL-c445t-8a33fb5c00a8b0334218acaee3fe1d0523aae88fafb48ae8f66ba6a70fa9b6b03</originalsourceid><addsrcrecordid>eNo90U1v1DAQBmALgehSuPADwAcOLVJg_JHYe4RCAakSSKUSt2iSHS9GSZz64wC_Hle77cmjmWfm8JqxlwLeCQDzfnAAsttKKx-xjdBKNhLUr8dsA0aKxhihT9izlP4A1KHpnrITqWWrrGk3bL3-HULmc5myXyc_YvZh4S6Gmc-YSySeI1HiwfFPoewnTI3zkZ_9SFR2IaeyRz7T8s9T8v6c47Ljd73IV79QZX4piU84DxSzxwXPn7MnDqdEL47vKbu5_Pzz4mtz9f3Lt4sPV82odZsbi0q5oR0B0A6glJbC4ohEypHYQSsVIlnr0A3a1sp13YAdGnC4Hbq6ccrODnfXGG4LpdzPPo00TbhQKKkXuhNKa9h2lb490DGGlCK5fo1-xvi3F9DfJdx_vLxPuOJXx7tlmGn3QO8jreDNEWAacXIRl9GnB2fbFrTWlb0-MIehx32s5OZaglD1k7Q0BtR_PPqNig</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1461344096</pqid></control><display><type>article</type><title>Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana)</title><source>SpringerNature Journals</source><creator>Gupta, P.K ; Durzan, D.J</creator><creatorcontrib>Gupta, P.K ; Durzan, D.J</creatorcontrib><description>Opening of apical and axillary buds of mature Douglas-fir and sugar pine trees was obtained on a newly formulated basal medium (DCR) without growth regulators. Elongation of buds was observed on 1/2 strength DCR with 0.3% activated charcoal (DCR-1). In sugar pine, multiple shoots were obtained when explants on DCR with 0.5 mg/1 BAP for 5-6 weeks were transferred to DCR-1 medium. On subculture, axillary buds again developed when shoots were cultured on DCR with 0.2 mg/1 BAP for Douglas-fir and 0.5 mg/1 BAP for sugar pine. These buds were again elongated on DCR-1 medium. By subculturing 7-10 shoots of Douglas-fir and 2-3 shoots of sugar pine, over 100 shoots can be obtained in a year.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/bf00269282</identifier><identifier>PMID: 24253875</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Artificial regeneration. Forest nurseries. Planting ; Biological and medical sciences ; Biotechnology ; Eukaryotic cell cultures ; Forestry ; Fundamental and applied biological sciences. Psychology ; In vitro propagation: entire plant regeneration from tissues and cell cultures ; Methods. Procedures. Technologies ; Pinus lambertiana ; Plant cells and fungal cells ; Plant physiology and development ; plant propagation ; Pseudotsuga menziesii ; shoots ; Tissue cultures, protoplasts ; Vegetative propagation. Micropropagation</subject><ispartof>Plant cell reports, 1985-08, Vol.4 (4), p.177-179</ispartof><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-8a33fb5c00a8b0334218acaee3fe1d0523aae88fafb48ae8f66ba6a70fa9b6b03</citedby><cites>FETCH-LOGICAL-c445t-8a33fb5c00a8b0334218acaee3fe1d0523aae88fafb48ae8f66ba6a70fa9b6b03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8550444$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24253875$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gupta, P.K</creatorcontrib><creatorcontrib>Durzan, D.J</creatorcontrib><title>Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana)</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>Opening of apical and axillary buds of mature Douglas-fir and sugar pine trees was obtained on a newly formulated basal medium (DCR) without growth regulators. Elongation of buds was observed on 1/2 strength DCR with 0.3% activated charcoal (DCR-1). In sugar pine, multiple shoots were obtained when explants on DCR with 0.5 mg/1 BAP for 5-6 weeks were transferred to DCR-1 medium. On subculture, axillary buds again developed when shoots were cultured on DCR with 0.2 mg/1 BAP for Douglas-fir and 0.5 mg/1 BAP for sugar pine. These buds were again elongated on DCR-1 medium. By subculturing 7-10 shoots of Douglas-fir and 2-3 shoots of sugar pine, over 100 shoots can be obtained in a year.</description><subject>Artificial regeneration. Forest nurseries. Planting</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Eukaryotic cell cultures</subject><subject>Forestry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In vitro propagation: entire plant regeneration from tissues and cell cultures</subject><subject>Methods. Procedures. Technologies</subject><subject>Pinus lambertiana</subject><subject>Plant cells and fungal cells</subject><subject>Plant physiology and development</subject><subject>plant propagation</subject><subject>Pseudotsuga menziesii</subject><subject>shoots</subject><subject>Tissue cultures, protoplasts</subject><subject>Vegetative propagation. Micropropagation</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><recordid>eNo90U1v1DAQBmALgehSuPADwAcOLVJg_JHYe4RCAakSSKUSt2iSHS9GSZz64wC_Hle77cmjmWfm8JqxlwLeCQDzfnAAsttKKx-xjdBKNhLUr8dsA0aKxhihT9izlP4A1KHpnrITqWWrrGk3bL3-HULmc5myXyc_YvZh4S6Gmc-YSySeI1HiwfFPoewnTI3zkZ_9SFR2IaeyRz7T8s9T8v6c47Ljd73IV79QZX4piU84DxSzxwXPn7MnDqdEL47vKbu5_Pzz4mtz9f3Lt4sPV82odZsbi0q5oR0B0A6glJbC4ohEypHYQSsVIlnr0A3a1sp13YAdGnC4Hbq6ccrODnfXGG4LpdzPPo00TbhQKKkXuhNKa9h2lb490DGGlCK5fo1-xvi3F9DfJdx_vLxPuOJXx7tlmGn3QO8jreDNEWAacXIRl9GnB2fbFrTWlb0-MIehx32s5OZaglD1k7Q0BtR_PPqNig</recordid><startdate>198508</startdate><enddate>198508</enddate><creator>Gupta, P.K</creator><creator>Durzan, D.J</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198508</creationdate><title>Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana)</title><author>Gupta, P.K ; Durzan, D.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-8a33fb5c00a8b0334218acaee3fe1d0523aae88fafb48ae8f66ba6a70fa9b6b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Artificial regeneration. Forest nurseries. Planting</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Eukaryotic cell cultures</topic><topic>Forestry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In vitro propagation: entire plant regeneration from tissues and cell cultures</topic><topic>Methods. Procedures. Technologies</topic><topic>Pinus lambertiana</topic><topic>Plant cells and fungal cells</topic><topic>Plant physiology and development</topic><topic>plant propagation</topic><topic>Pseudotsuga menziesii</topic><topic>shoots</topic><topic>Tissue cultures, protoplasts</topic><topic>Vegetative propagation. Micropropagation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gupta, P.K</creatorcontrib><creatorcontrib>Durzan, D.J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gupta, P.K</au><au>Durzan, D.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana)</atitle><jtitle>Plant cell reports</jtitle><addtitle>Plant Cell Rep</addtitle><date>1985-08</date><risdate>1985</risdate><volume>4</volume><issue>4</issue><spage>177</spage><epage>179</epage><pages>177-179</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>Opening of apical and axillary buds of mature Douglas-fir and sugar pine trees was obtained on a newly formulated basal medium (DCR) without growth regulators. Elongation of buds was observed on 1/2 strength DCR with 0.3% activated charcoal (DCR-1). In sugar pine, multiple shoots were obtained when explants on DCR with 0.5 mg/1 BAP for 5-6 weeks were transferred to DCR-1 medium. On subculture, axillary buds again developed when shoots were cultured on DCR with 0.2 mg/1 BAP for Douglas-fir and 0.5 mg/1 BAP for sugar pine. These buds were again elongated on DCR-1 medium. By subculturing 7-10 shoots of Douglas-fir and 2-3 shoots of sugar pine, over 100 shoots can be obtained in a year.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>24253875</pmid><doi>10.1007/bf00269282</doi><tpages>3</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0721-7714 |
ispartof | Plant cell reports, 1985-08, Vol.4 (4), p.177-179 |
issn | 0721-7714 1432-203X |
language | eng |
recordid | cdi_proquest_miscellaneous_1461344096 |
source | SpringerNature Journals |
subjects | Artificial regeneration. Forest nurseries. Planting Biological and medical sciences Biotechnology Eukaryotic cell cultures Forestry Fundamental and applied biological sciences. Psychology In vitro propagation: entire plant regeneration from tissues and cell cultures Methods. Procedures. Technologies Pinus lambertiana Plant cells and fungal cells Plant physiology and development plant propagation Pseudotsuga menziesii shoots Tissue cultures, protoplasts Vegetative propagation. Micropropagation |
title | Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana) |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T18%3A34%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Shoot%20multiplication%20from%20mature%20trees%20of%20Douglas-fir%20(Pseudotsuga%20menziesii)%20and%20sugar%20pine%20(Pinus%20lambertiana)&rft.jtitle=Plant%20cell%20reports&rft.au=Gupta,%20P.K&rft.date=1985-08&rft.volume=4&rft.issue=4&rft.spage=177&rft.epage=179&rft.pages=177-179&rft.issn=0721-7714&rft.eissn=1432-203X&rft.coden=PCRPD8&rft_id=info:doi/10.1007/bf00269282&rft_dat=%3Cproquest_cross%3E1461344096%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1461344096&rft_id=info:pmid/24253875&rfr_iscdi=true |