Efficient expression of truncated recombinant cadmium-metallothionein gene of a ciliate, Tetrahymena tropicalis lahorensis in Escherichia coli

Truncated recombinant metallothionein GST–fusion protein has been successfully expressed in Escherichia coli . The previously identified novel Cd-inducible metallothionein ( TMCd1 ) gene from the locally isolated ciliate, Tetrahymena tropicalis lahorensis , was inserted into a pET-41a vector, in fra...

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Veröffentlicht in:	Molecular biology reports 2013-12, Vol.40 (12), p.7061-7068
Hauptverfasser:	Shuja, Rukhsana Nighat, Taimuri, Shuja Uddin Ahmad, Shakoori, Farah Rauf, Shakoori, Abdul Rauf
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Anatomy Animal Biochemistry Biomedical and Life Sciences Cadmium Chromatography E coli Electrophoresis, Polyacrylamide Gel Escherichia coli - metabolism Gene Expression Histology Life Sciences Metallothionein - chemistry Metallothionein - genetics Models, Molecular Molecular biology Morphology Polymerase Chain Reaction Proteins Recombinant Fusion Proteins - metabolism Tetrahymena - genetics
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