Expression and purification of ecdysteroid-regulated protein from Chinese mitten crab Eriocheir sinensis in E. coli

The glutathione S-transferase (GST) fusion protein system is widely used for high-level expression and efficient purification of recombinant proteins from bacteria. The goal of this study was to clone, efficiently express and purify the ecdysteroid-regulated protein (ERP) in the form of a GST fusion...

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Veröffentlicht in:	Molecular biology reports 2013-12, Vol.40 (12), p.6987-6995
Hauptverfasser:	He, Chongbo, Chen, Panhai, Gao, Xianggang, Gao, Lei, Li, Le
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Arthropod Proteins - chemistry Arthropod Proteins - isolation & purification Arthropod Proteins - metabolism Base Sequence Brachyura - metabolism China Chromatography, Liquid Cloning, Molecular DNA - metabolism DNA, Complementary - genetics Ecdysteroids - metabolism Escherichia coli - metabolism Genetic Vectors - metabolism Mass Spectrometry Molecular Sequence Data Phylogeny Protein Structure, Tertiary Recombinant Fusion Proteins - isolation & purification Recombinant Fusion Proteins - metabolism Sequence Alignment Sequence Analysis, DNA
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