Cell regeneration and sustained division of protoplasts from cotton (Gossypium hirsutum L.)
Protoplasts were isolated from 12 day old subcultured phytohormone habituated callus tissue of Gossypium hirsutum L. (0.5% cellulysin-Calbiochem, 0.6% macerase-Calbiochem, 0.7M mannitol, and pH 5.0). After separation and purification (0.35M sucrose floatation medium), the protoplasts were cultured (...
Gespeichert in:
| Veröffentlicht in: | Plant cell reports 1987-12, Vol.6 (6), p.470-472 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 472 |
|---|---|
| container_issue | 6 |
| container_start_page | 470 |
| container_title | Plant cell reports |
| container_volume | 6 |
| creator | Saka, K Katterman, F.R Thomas, J.C |
| description | Protoplasts were isolated from 12 day old subcultured phytohormone habituated callus tissue of Gossypium hirsutum L. (0.5% cellulysin-Calbiochem, 0.6% macerase-Calbiochem, 0.7M mannitol, and pH 5.0). After separation and purification (0.35M sucrose floatation medium), the protoplasts were cultured (K3 media of Kao et al., 1974 with 0.9 μM BAP, 5 μM IAA and 0.35M sucrose) in both liquid and solid medium at a density of 5×10(5) protoplasts/ml. Four weeks after isolation, cell regeneration and callus formation was observed. |
| doi_str_mv | 10.1007/BF00272785 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1459975680</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1459975680</sourcerecordid><originalsourceid>FETCH-LOGICAL-c338t-e1c973de492412abfc19f8897bad4bc68b020f411430683efcb4f4b02cb5b0003</originalsourceid><addsrcrecordid>eNpF0E1P3DAQBmALFbHbLRd-AM2hB4oUOv5IbB_LqkuRVuJAkSpxiBzHBqMkXmwHaf89Xu2WnmzNPBq9MwidYbjCAPzH9QqAcMJFdYTmmFFSEqB_P6E5cIJLzjGboc8xvgDkJq9P0IwwwoSk1Rw9Lk3fF8E8mdEElZwfCzV2RZxiUm40XdG5Nxd3ZW-LTfDJb3oVUyxs8EOhfUq5dXHjY9xu3DQUzy7EKeXP-ur7F3RsVR_N6eFdoIfVrz_L3-X67uZ2-XNdakpFKg3WktPOMEkYJqq1GksrhOSt6lira9ECActwDg-1oMbqllmWi7qtWgCgC3Sxn5vzvU4mpmZwUee91Gj8FBvMKil5VYsdvdxTHXLkYGyzCW5QYdtgaHbHbP4fM-Pzw9ypHUz3Qf9dL4NvB6CiVr0NatQufjheSywIyezrnlnlG_UUMnm4J4Bp3otTKQl9ByighOg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1459975680</pqid></control><display><type>article</type><title>Cell regeneration and sustained division of protoplasts from cotton (Gossypium hirsutum L.)</title><source>SpringerLink Journals</source><creator>Saka, K ; Katterman, F.R ; Thomas, J.C</creator><creatorcontrib>Saka, K ; Katterman, F.R ; Thomas, J.C</creatorcontrib><description>Protoplasts were isolated from 12 day old subcultured phytohormone habituated callus tissue of Gossypium hirsutum L. (0.5% cellulysin-Calbiochem, 0.6% macerase-Calbiochem, 0.7M mannitol, and pH 5.0). After separation and purification (0.35M sucrose floatation medium), the protoplasts were cultured (K3 media of Kao et al., 1974 with 0.9 μM BAP, 5 μM IAA and 0.35M sucrose) in both liquid and solid medium at a density of 5×10(5) protoplasts/ml. Four weeks after isolation, cell regeneration and callus formation was observed.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/BF00272785</identifier><identifier>PMID: 24248935</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Agronomy. Soil science and plant productions ; Biological and medical sciences ; Biotechnology ; callus ; Cell cultures. Hybridization. Fusion ; cell division ; cell growth ; Economic plant physiology ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; Gossypium hirsutum ; In vitro culture ; Methods. Procedures. Technologies ; Molecular and cellular biology ; Plant cells and fungal cells ; Protoplast preparation, cell fusion and regeneration ; protoplasts ; regeneration</subject><ispartof>Plant cell reports, 1987-12, Vol.6 (6), p.470-472</ispartof><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c338t-e1c973de492412abfc19f8897bad4bc68b020f411430683efcb4f4b02cb5b0003</citedby><cites>FETCH-LOGICAL-c338t-e1c973de492412abfc19f8897bad4bc68b020f411430683efcb4f4b02cb5b0003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7691822$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24248935$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saka, K</creatorcontrib><creatorcontrib>Katterman, F.R</creatorcontrib><creatorcontrib>Thomas, J.C</creatorcontrib><title>Cell regeneration and sustained division of protoplasts from cotton (Gossypium hirsutum L.)</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>Protoplasts were isolated from 12 day old subcultured phytohormone habituated callus tissue of Gossypium hirsutum L. (0.5% cellulysin-Calbiochem, 0.6% macerase-Calbiochem, 0.7M mannitol, and pH 5.0). After separation and purification (0.35M sucrose floatation medium), the protoplasts were cultured (K3 media of Kao et al., 1974 with 0.9 μM BAP, 5 μM IAA and 0.35M sucrose) in both liquid and solid medium at a density of 5×10(5) protoplasts/ml. Four weeks after isolation, cell regeneration and callus formation was observed.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>callus</subject><subject>Cell cultures. Hybridization. Fusion</subject><subject>cell division</subject><subject>cell growth</subject><subject>Economic plant physiology</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gossypium hirsutum</subject><subject>In vitro culture</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular and cellular biology</subject><subject>Plant cells and fungal cells</subject><subject>Protoplast preparation, cell fusion and regeneration</subject><subject>protoplasts</subject><subject>regeneration</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNpF0E1P3DAQBmALFbHbLRd-AM2hB4oUOv5IbB_LqkuRVuJAkSpxiBzHBqMkXmwHaf89Xu2WnmzNPBq9MwidYbjCAPzH9QqAcMJFdYTmmFFSEqB_P6E5cIJLzjGboc8xvgDkJq9P0IwwwoSk1Rw9Lk3fF8E8mdEElZwfCzV2RZxiUm40XdG5Nxd3ZW-LTfDJb3oVUyxs8EOhfUq5dXHjY9xu3DQUzy7EKeXP-ur7F3RsVR_N6eFdoIfVrz_L3-X67uZ2-XNdakpFKg3WktPOMEkYJqq1GksrhOSt6lira9ECActwDg-1oMbqllmWi7qtWgCgC3Sxn5vzvU4mpmZwUee91Gj8FBvMKil5VYsdvdxTHXLkYGyzCW5QYdtgaHbHbP4fM-Pzw9ypHUz3Qf9dL4NvB6CiVr0NatQufjheSywIyezrnlnlG_UUMnm4J4Bp3otTKQl9ByighOg</recordid><startdate>19871201</startdate><enddate>19871201</enddate><creator>Saka, K</creator><creator>Katterman, F.R</creator><creator>Thomas, J.C</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19871201</creationdate><title>Cell regeneration and sustained division of protoplasts from cotton (Gossypium hirsutum L.)</title><author>Saka, K ; Katterman, F.R ; Thomas, J.C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-e1c973de492412abfc19f8897bad4bc68b020f411430683efcb4f4b02cb5b0003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>callus</topic><topic>Cell cultures. Hybridization. Fusion</topic><topic>cell division</topic><topic>cell growth</topic><topic>Economic plant physiology</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gossypium hirsutum</topic><topic>In vitro culture</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular and cellular biology</topic><topic>Plant cells and fungal cells</topic><topic>Protoplast preparation, cell fusion and regeneration</topic><topic>protoplasts</topic><topic>regeneration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saka, K</creatorcontrib><creatorcontrib>Katterman, F.R</creatorcontrib><creatorcontrib>Thomas, J.C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saka, K</au><au>Katterman, F.R</au><au>Thomas, J.C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell regeneration and sustained division of protoplasts from cotton (Gossypium hirsutum L.)</atitle><jtitle>Plant cell reports</jtitle><addtitle>Plant Cell Rep</addtitle><date>1987-12-01</date><risdate>1987</risdate><volume>6</volume><issue>6</issue><spage>470</spage><epage>472</epage><pages>470-472</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>Protoplasts were isolated from 12 day old subcultured phytohormone habituated callus tissue of Gossypium hirsutum L. (0.5% cellulysin-Calbiochem, 0.6% macerase-Calbiochem, 0.7M mannitol, and pH 5.0). After separation and purification (0.35M sucrose floatation medium), the protoplasts were cultured (K3 media of Kao et al., 1974 with 0.9 μM BAP, 5 μM IAA and 0.35M sucrose) in both liquid and solid medium at a density of 5×10(5) protoplasts/ml. Four weeks after isolation, cell regeneration and callus formation was observed.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>24248935</pmid><doi>10.1007/BF00272785</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0721-7714 |
| ispartof | Plant cell reports, 1987-12, Vol.6 (6), p.470-472 |
| issn | 0721-7714 1432-203X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1459975680 |
| source | SpringerLink Journals |
| subjects | Agronomy. Soil science and plant productions Biological and medical sciences Biotechnology callus Cell cultures. Hybridization. Fusion cell division cell growth Economic plant physiology Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Gossypium hirsutum In vitro culture Methods. Procedures. Technologies Molecular and cellular biology Plant cells and fungal cells Protoplast preparation, cell fusion and regeneration protoplasts regeneration |
| title | Cell regeneration and sustained division of protoplasts from cotton (Gossypium hirsutum L.) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T11%3A30%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cell%20regeneration%20and%20sustained%20division%20of%20protoplasts%20from%20cotton%20(Gossypium%20hirsutum%20L.)&rft.jtitle=Plant%20cell%20reports&rft.au=Saka,%20K&rft.date=1987-12-01&rft.volume=6&rft.issue=6&rft.spage=470&rft.epage=472&rft.pages=470-472&rft.issn=0721-7714&rft.eissn=1432-203X&rft.coden=PCRPD8&rft_id=info:doi/10.1007/BF00272785&rft_dat=%3Cproquest_cross%3E1459975680%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1459975680&rft_id=info:pmid/24248935&rfr_iscdi=true |