Genetic transformation of flax (Linum usitatissimum) by Agrobacterium tumefaciens: regeneration of transformed shoots via a callus phase
Genetic transformation of flax (Linum usitatissimum) has been achieved using an A. tumefaciens strain carrying a non-oncogenic Ti plasmid-derived vector containing a chimaeric npt-II gene and a wild type nopaline synthase gene. Fertile, transformed shoots were most easily obtained from Km(r) callus...
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| Veröffentlicht in: | Plant cell reports 1987-10, Vol.6 (5), p.396-399 |
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| creator | Basiran, N Armitage, P Scott, R.J Draper, J |
| description | Genetic transformation of flax (Linum usitatissimum) has been achieved using an A. tumefaciens strain carrying a non-oncogenic Ti plasmid-derived vector containing a chimaeric npt-II gene and a wild type nopaline synthase gene. Fertile, transformed shoots were most easily obtained from Km(r) callus developing on hypocotyl sections. The totipotency of the Km(r) callus was dependent upon its origin. T-DNA was visualised by Southern blotting in all Km(r) tissues. Efficient expression of nopaline synthase and the chimaeric npt-II gene was found in transformed Km(r) callus and regenerated shoots. |
| doi_str_mv | 10.1007/bf00269571 |
| format | Article |
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Fertile, transformed shoots were most easily obtained from Km(r) callus developing on hypocotyl sections. The totipotency of the Km(r) callus was dependent upon its origin. T-DNA was visualised by Southern blotting in all Km(r) tissues. Efficient expression of nopaline synthase and the chimaeric npt-II gene was found in transformed Km(r) callus and regenerated shoots.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/bf00269571</identifier><identifier>PMID: 24248856</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Agrobacterium tumefaciens ; Agronomy. Soil science and plant productions ; Biological and medical sciences ; Biotechnology ; explants ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic engineering applications ; Genetic technics ; genetic transformation ; Genetics and breeding of economic plants ; Linum usitatissimum ; Methods. Procedures. Technologies ; Plant breeding: fundamental aspects and methodology ; tissue culture ; Vectors (cloning, transfer, expression). 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Fertile, transformed shoots were most easily obtained from Km(r) callus developing on hypocotyl sections. The totipotency of the Km(r) callus was dependent upon its origin. T-DNA was visualised by Southern blotting in all Km(r) tissues. Efficient expression of nopaline synthase and the chimaeric npt-II gene was found in transformed Km(r) callus and regenerated shoots.</description><subject>Agrobacterium tumefaciens</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>explants</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic engineering applications</subject><subject>Genetic technics</subject><subject>genetic transformation</subject><subject>Genetics and breeding of economic plants</subject><subject>Linum usitatissimum</subject><subject>Methods. Procedures. Technologies</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>tissue culture</subject><subject>Vectors (cloning, transfer, expression). Insertion sequences and transposons</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNo9kMFu1DAQhi0EotvChQcAHzgUpIDtOHHCra1oQVqJA1TiFo29461REm89DqJvwGNjtNs9zeH_9EnzMfZKig9SCPPReiFU2zdGPmErqWtVKVH_fMpWwihZGSP1CTsl-iVEGU37nJ0orXTXNe2K_b3BGXNwPCeYycc0QQ5x5tFzP8Iffr4O8zLxhUIuA1GYlukdtw_8YpuiBZcxhbLnZUIPLuBMn3jCbZGmo-ioxg2nuxgz8d8BOHAH47gQ390B4Qv2zMNI-PJwz9jt9ecfV1-q9bebr1cX68ppoXMF0Hu00JnOWWm0BLdBp2uL4HxtTQ3grNrUGo1zDQjbtN6CMtI6lFZ2XX3GzvfeXYr3C1IepkAOxxFmjAsNUjd9b7TWsqDv96hLkSihH3YpTJAeBimG_-WHy-vH8gV-ffAutjx6RB9TF-DtAQAqj_sSxQU6csYIJXpTsDd7zEMcYJsKcvtdCVmXuTWlQf0PaIOYYw</recordid><startdate>19871001</startdate><enddate>19871001</enddate><creator>Basiran, N</creator><creator>Armitage, P</creator><creator>Scott, R.J</creator><creator>Draper, J</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19871001</creationdate><title>Genetic transformation of flax (Linum usitatissimum) by Agrobacterium tumefaciens: regeneration of transformed shoots via a callus phase</title><author>Basiran, N ; Armitage, P ; Scott, R.J ; Draper, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-aa9feba878cb1741acdec43beacf3b73aacb2d34e7cc5a0b56fba271bce1b1883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Agrobacterium tumefaciens</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>explants</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic engineering applications</topic><topic>Genetic technics</topic><topic>genetic transformation</topic><topic>Genetics and breeding of economic plants</topic><topic>Linum usitatissimum</topic><topic>Methods. Procedures. Technologies</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>tissue culture</topic><topic>Vectors (cloning, transfer, expression). Insertion sequences and transposons</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Basiran, N</creatorcontrib><creatorcontrib>Armitage, P</creatorcontrib><creatorcontrib>Scott, R.J</creatorcontrib><creatorcontrib>Draper, J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Basiran, N</au><au>Armitage, P</au><au>Scott, R.J</au><au>Draper, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic transformation of flax (Linum usitatissimum) by Agrobacterium tumefaciens: regeneration of transformed shoots via a callus phase</atitle><jtitle>Plant cell reports</jtitle><addtitle>Plant Cell Rep</addtitle><date>1987-10-01</date><risdate>1987</risdate><volume>6</volume><issue>5</issue><spage>396</spage><epage>399</epage><pages>396-399</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>Genetic transformation of flax (Linum usitatissimum) has been achieved using an A. tumefaciens strain carrying a non-oncogenic Ti plasmid-derived vector containing a chimaeric npt-II gene and a wild type nopaline synthase gene. Fertile, transformed shoots were most easily obtained from Km(r) callus developing on hypocotyl sections. The totipotency of the Km(r) callus was dependent upon its origin. T-DNA was visualised by Southern blotting in all Km(r) tissues. Efficient expression of nopaline synthase and the chimaeric npt-II gene was found in transformed Km(r) callus and regenerated shoots.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>24248856</pmid><doi>10.1007/bf00269571</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 0721-7714 |
| ispartof | Plant cell reports, 1987-10, Vol.6 (5), p.396-399 |
| issn | 0721-7714 1432-203X |
| language | eng |
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| source | SpringerLink Journals |
| subjects | Agrobacterium tumefaciens Agronomy. Soil science and plant productions Biological and medical sciences Biotechnology explants Fundamental and applied biological sciences. Psychology Genetic engineering Genetic engineering applications Genetic technics genetic transformation Genetics and breeding of economic plants Linum usitatissimum Methods. Procedures. Technologies Plant breeding: fundamental aspects and methodology tissue culture Vectors (cloning, transfer, expression). Insertion sequences and transposons |
| title | Genetic transformation of flax (Linum usitatissimum) by Agrobacterium tumefaciens: regeneration of transformed shoots via a callus phase |
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