Gas chromatography-mass spectrometry evidence for several endogenous auxins in pea seedling organs
Qualitative analysis by gas chromatography-mass spectrometry (GC-MS) of the auxins present in the root, cotyledons and epicotyl of 3-d-old etiolated pea (Pisum sativum L., cv. Alaska) seedlings has shown that all three organs contain phenylacetic acid (PAA), 3-indoleacetic acid (IAA) and 4-chloro-3-...
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Veröffentlicht in: | Planta 1985-01, Vol.165 (2), p.232-241 |
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description | Qualitative analysis by gas chromatography-mass spectrometry (GC-MS) of the auxins present in the root, cotyledons and epicotyl of 3-d-old etiolated pea (Pisum sativum L., cv. Alaska) seedlings has shown that all three organs contain phenylacetic acid (PAA), 3-indoleacetic acid (IAA) and 4-chloro-3-indoleacetic acid (4Cl-IAA). In addition, 3-indolepropionic acid (IPA) was present in the root and 3-indolebutyric acid (IBA) was detected in both root and epicotyl. Phenylacetic acid, IAA and IPA were measured quantitatively in the three organs by GC-MS-single ion monitoring, using deuterated internal standards. Levels of IAA were found to range from 13 to 115 pmol g-1 FW, while amounts of PAA were considerably higher (347—451 pmol g-1 FW) and the level of IPA was quite low (5 pmol g-1 FW). On a molar basis the PAA:IAA ratio in the whole seedling was approx. 15:1. |
doi_str_mv | 10.1007/bf00395046 |
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Alaska) seedlings has shown that all three organs contain phenylacetic acid (PAA), 3-indoleacetic acid (IAA) and 4-chloro-3-indoleacetic acid (4Cl-IAA). In addition, 3-indolepropionic acid (IPA) was present in the root and 3-indolebutyric acid (IBA) was detected in both root and epicotyl. Phenylacetic acid, IAA and IPA were measured quantitatively in the three organs by GC-MS-single ion monitoring, using deuterated internal standards. Levels of IAA were found to range from 13 to 115 pmol g-1 FW, while amounts of PAA were considerably higher (347—451 pmol g-1 FW) and the level of IPA was quite low (5 pmol g-1 FW). On a molar basis the PAA:IAA ratio in the whole seedling was approx. 15:1.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/bf00395046</identifier><identifier>PMID: 24241048</identifier><language>eng</language><publisher>Germany: Springer-Verlag</publisher><subject>Auxins ; chemical constituents of plants ; Cotyledons ; Epicotyls ; Esters ; Ethers ; gas chromatography ; Mass spectra ; mass spectrometry ; Peas ; Pisum sativum ; Plant roots ; Plants ; Seedlings</subject><ispartof>Planta, 1985-01, Vol.165 (2), p.232-241</ispartof><rights>Springer-Verlag Berlin Heidelberg 1985</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-e1f7092de5af8bd2b97278098cbcec006ca81d25f43eb0a3a340b1cfa96a6fb53</citedby><cites>FETCH-LOGICAL-c438t-e1f7092de5af8bd2b97278098cbcec006ca81d25f43eb0a3a340b1cfa96a6fb53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23377779$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23377779$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24241048$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schneider, E.A</creatorcontrib><creatorcontrib>Kazakoff, C.W</creatorcontrib><creatorcontrib>Wightman, F</creatorcontrib><title>Gas chromatography-mass spectrometry evidence for several endogenous auxins in pea seedling organs</title><title>Planta</title><addtitle>Planta</addtitle><description>Qualitative analysis by gas chromatography-mass spectrometry (GC-MS) of the auxins present in the root, cotyledons and epicotyl of 3-d-old etiolated pea (Pisum sativum L., cv. Alaska) seedlings has shown that all three organs contain phenylacetic acid (PAA), 3-indoleacetic acid (IAA) and 4-chloro-3-indoleacetic acid (4Cl-IAA). In addition, 3-indolepropionic acid (IPA) was present in the root and 3-indolebutyric acid (IBA) was detected in both root and epicotyl. Phenylacetic acid, IAA and IPA were measured quantitatively in the three organs by GC-MS-single ion monitoring, using deuterated internal standards. Levels of IAA were found to range from 13 to 115 pmol g-1 FW, while amounts of PAA were considerably higher (347—451 pmol g-1 FW) and the level of IPA was quite low (5 pmol g-1 FW). On a molar basis the PAA:IAA ratio in the whole seedling was approx. 15:1.</description><subject>Auxins</subject><subject>chemical constituents of plants</subject><subject>Cotyledons</subject><subject>Epicotyls</subject><subject>Esters</subject><subject>Ethers</subject><subject>gas chromatography</subject><subject>Mass spectra</subject><subject>mass spectrometry</subject><subject>Peas</subject><subject>Pisum sativum</subject><subject>Plant roots</subject><subject>Plants</subject><subject>Seedlings</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><recordid>eNpFkUtv1TAQRi0EoreFDXvAywopMH7ktSwVLUiVWEDX0dgZp6mSOHhyK-6_x-i2sBrpO0ej0TdCvFHwUQHUn1wAMG0JtnomdsoaXWiwzXOxy7EuoDXliThlvgfIsK5fihNttVXZ2Ql3jSz9XYozbnFIuN4dihmZJa_ktxzTlg6SHsaeFk8yxCSZHijhJGnp40BL3LPE_e9xYTkuciXMAvXTuAwypgEXfiVeBJyYXj_OM3F79eXn5dfi5vv1t8uLm8Jb02wFqVBDq3sqMTSu166tdd1A23jnyQNUHhvV6zJYQw7QoLHglA_YVlgFV5ozcX7cu6b4a0-8dfPInqYJF8pHdsqWbVkZ1VRZ_XBUfYrMiUK3pnHGdOgUdH877T5fPXWa5XePe_dupv6f-lRiFt4ehXveYvrPTS67rtvM3x95wNjhkEbubn9oUAZ0VZr8HvMHUX-GCw</recordid><startdate>19850101</startdate><enddate>19850101</enddate><creator>Schneider, E.A</creator><creator>Kazakoff, C.W</creator><creator>Wightman, F</creator><general>Springer-Verlag</general><scope>FBQ</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19850101</creationdate><title>Gas chromatography-mass spectrometry evidence for several endogenous auxins in pea seedling organs</title><author>Schneider, E.A ; Kazakoff, C.W ; Wightman, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-e1f7092de5af8bd2b97278098cbcec006ca81d25f43eb0a3a340b1cfa96a6fb53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Auxins</topic><topic>chemical constituents of plants</topic><topic>Cotyledons</topic><topic>Epicotyls</topic><topic>Esters</topic><topic>Ethers</topic><topic>gas chromatography</topic><topic>Mass spectra</topic><topic>mass spectrometry</topic><topic>Peas</topic><topic>Pisum sativum</topic><topic>Plant roots</topic><topic>Plants</topic><topic>Seedlings</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schneider, E.A</creatorcontrib><creatorcontrib>Kazakoff, C.W</creatorcontrib><creatorcontrib>Wightman, F</creatorcontrib><collection>AGRIS</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schneider, E.A</au><au>Kazakoff, C.W</au><au>Wightman, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gas chromatography-mass spectrometry evidence for several endogenous auxins in pea seedling organs</atitle><jtitle>Planta</jtitle><addtitle>Planta</addtitle><date>1985-01-01</date><risdate>1985</risdate><volume>165</volume><issue>2</issue><spage>232</spage><epage>241</epage><pages>232-241</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><abstract>Qualitative analysis by gas chromatography-mass spectrometry (GC-MS) of the auxins present in the root, cotyledons and epicotyl of 3-d-old etiolated pea (Pisum sativum L., cv. Alaska) seedlings has shown that all three organs contain phenylacetic acid (PAA), 3-indoleacetic acid (IAA) and 4-chloro-3-indoleacetic acid (4Cl-IAA). In addition, 3-indolepropionic acid (IPA) was present in the root and 3-indolebutyric acid (IBA) was detected in both root and epicotyl. Phenylacetic acid, IAA and IPA were measured quantitatively in the three organs by GC-MS-single ion monitoring, using deuterated internal standards. Levels of IAA were found to range from 13 to 115 pmol g-1 FW, while amounts of PAA were considerably higher (347—451 pmol g-1 FW) and the level of IPA was quite low (5 pmol g-1 FW). On a molar basis the PAA:IAA ratio in the whole seedling was approx. 15:1.</abstract><cop>Germany</cop><pub>Springer-Verlag</pub><pmid>24241048</pmid><doi>10.1007/bf00395046</doi><tpages>10</tpages></addata></record> |
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subjects | Auxins chemical constituents of plants Cotyledons Epicotyls Esters Ethers gas chromatography Mass spectra mass spectrometry Peas Pisum sativum Plant roots Plants Seedlings |
title | Gas chromatography-mass spectrometry evidence for several endogenous auxins in pea seedling organs |
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