Enzymatic reduction of (+)-dihydroflavonols to flavan-3,4-cis-diols with flower extracts from Matthiola incana and its role in anthocyanin biosynthesis
A cell-free extract from flowers of Matthiola incana catalyzes a NADPH-dependent stereospecific reduction of (+)-dihydrokaempferol to 3,4-cis-leucopelargonidin (5,7,4′-trihydroxyflavan-3,4-cis-diol). The pH-optimum of this reaction is around 6. The rate of reaction with NADH was about 50% of that fo...
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| Veröffentlicht in: | Planta 1985-01, Vol.165 (2), p.284-287 |
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| creator | Heller, W Forkmann, G Britsch, L Grisebach, H |
| description | A cell-free extract from flowers of Matthiola incana catalyzes a NADPH-dependent stereospecific reduction of (+)-dihydrokaempferol to 3,4-cis-leucopelargonidin (5,7,4′-trihydroxyflavan-3,4-cis-diol). The pH-optimum of this reaction is around 6. The rate of reaction with NADH was about 50% of that found with NADPH. (+)-Dihydroquercetin and (+)-dihydromyricetin were also reduced by the enzyme preparation to the corresponding flavan-3,4-cis-diols. Correlation between the genotype of M. incana and the presence of dihydroflavonol 4-reductase is strong evidence that this enzyme is involved in anthocyanin biosynthesis. |
| doi_str_mv | 10.1007/BF00395052 |
| format | Article |
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The pH-optimum of this reaction is around 6. The rate of reaction with NADH was about 50% of that found with NADPH. (+)-Dihydroquercetin and (+)-dihydromyricetin were also reduced by the enzyme preparation to the corresponding flavan-3,4-cis-diols. Correlation between the genotype of M. incana and the presence of dihydroflavonol 4-reductase is strong evidence that this enzyme is involved in anthocyanin biosynthesis.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/BF00395052</identifier><identifier>PMID: 24241054</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin: Springer-Verlag</publisher><subject>Alleles ; Analytical, structural and metabolic biochemistry ; anthocyanins ; Aromatic and heterocyclic compounds ; Biological and medical sciences ; Biosynthesis ; Chromatography ; Enzyme activity ; Enzyme preparations ; Enzymes ; Flavonoids ; flavonols ; Flowers ; Fundamental and applied biological sciences. Psychology ; Genotypes ; Heterocyclic compounds, pigments ; Matthiola incana ; Other biological molecules ; plant extracts ; reduction ; Solvents</subject><ispartof>Planta, 1985-01, Vol.165 (2), p.284-287</ispartof><rights>Springer-Verlag Berlin Heidelberg 1985</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-2c5b35f330e77da80d5c4b923e55eccc2ba8bf7348c0b6541ac5263819e67be3</citedby><cites>FETCH-LOGICAL-c360t-2c5b35f330e77da80d5c4b923e55eccc2ba8bf7348c0b6541ac5263819e67be3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23377785$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23377785$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8568268$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24241054$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Heller, W</creatorcontrib><creatorcontrib>Forkmann, G</creatorcontrib><creatorcontrib>Britsch, L</creatorcontrib><creatorcontrib>Grisebach, H</creatorcontrib><title>Enzymatic reduction of (+)-dihydroflavonols to flavan-3,4-cis-diols with flower extracts from Matthiola incana and its role in anthocyanin biosynthesis</title><title>Planta</title><addtitle>Planta</addtitle><description>A cell-free extract from flowers of Matthiola incana catalyzes a NADPH-dependent stereospecific reduction of (+)-dihydrokaempferol to 3,4-cis-leucopelargonidin (5,7,4′-trihydroxyflavan-3,4-cis-diol). The pH-optimum of this reaction is around 6. The rate of reaction with NADH was about 50% of that found with NADPH. (+)-Dihydroquercetin and (+)-dihydromyricetin were also reduced by the enzyme preparation to the corresponding flavan-3,4-cis-diols. Correlation between the genotype of M. incana and the presence of dihydroflavonol 4-reductase is strong evidence that this enzyme is involved in anthocyanin biosynthesis.</description><subject>Alleles</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>anthocyanins</subject><subject>Aromatic and heterocyclic compounds</subject><subject>Biological and medical sciences</subject><subject>Biosynthesis</subject><subject>Chromatography</subject><subject>Enzyme activity</subject><subject>Enzyme preparations</subject><subject>Enzymes</subject><subject>Flavonoids</subject><subject>flavonols</subject><subject>Flowers</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotypes</subject><subject>Heterocyclic compounds, pigments</subject><subject>Matthiola incana</subject><subject>Other biological molecules</subject><subject>plant extracts</subject><subject>reduction</subject><subject>Solvents</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><recordid>eNpFkUtv1DAUhS0EotPChj3gBYvyCPiZOMtStYBUxIKyjm4ch3GV2K3toYQ_wt_ljmZoV_Y959PR1T2EPOPsPWes-fDxnDHZaqbFA7LiSopKMGUekhXKomKt1AfkMOcrxtBsmsfkQCihONNqRf6ehT_LDMVbmtywscXHQONIj9--rga_XoYUxwl-xRCnTEuk2wFCJd-pyvqMyFa_9WWNTrx1ibrfJYEtmY4pzvQrlLJGBqgPFgJQCAP16KY4OdRwLutoFwj4733MC84u-_yEPBphyu7p_j0il-dnl6efq4tvn76cnlxUVtasVMLqXupRSuaaZgDDBm1V3wrptHbWWtGD6cdGKmNZX2vFwWpRS8NbVze9k0fkeBd7neLNxuXSzT5bN00QXNzkjivd6loyoRB9s0NtijknN3bXyc-Qlo6zbttDd98Dwi_2uZt-dsMd-v_wCLzaA5AtTGOCgPe844yujagNYs932FUuMd3HSOyxMRr9lzt_hNjBz4QRP74LxnHjWstWcfkP7m2j3g</recordid><startdate>19850101</startdate><enddate>19850101</enddate><creator>Heller, W</creator><creator>Forkmann, G</creator><creator>Britsch, L</creator><creator>Grisebach, H</creator><general>Springer-Verlag</general><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19850101</creationdate><title>Enzymatic reduction of (+)-dihydroflavonols to flavan-3,4-cis-diols with flower extracts from Matthiola incana and its role in anthocyanin biosynthesis</title><author>Heller, W ; Forkmann, G ; Britsch, L ; Grisebach, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-2c5b35f330e77da80d5c4b923e55eccc2ba8bf7348c0b6541ac5263819e67be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Alleles</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>anthocyanins</topic><topic>Aromatic and heterocyclic compounds</topic><topic>Biological and medical sciences</topic><topic>Biosynthesis</topic><topic>Chromatography</topic><topic>Enzyme activity</topic><topic>Enzyme preparations</topic><topic>Enzymes</topic><topic>Flavonoids</topic><topic>flavonols</topic><topic>Flowers</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotypes</topic><topic>Heterocyclic compounds, pigments</topic><topic>Matthiola incana</topic><topic>Other biological molecules</topic><topic>plant extracts</topic><topic>reduction</topic><topic>Solvents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heller, W</creatorcontrib><creatorcontrib>Forkmann, G</creatorcontrib><creatorcontrib>Britsch, L</creatorcontrib><creatorcontrib>Grisebach, H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heller, W</au><au>Forkmann, G</au><au>Britsch, L</au><au>Grisebach, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzymatic reduction of (+)-dihydroflavonols to flavan-3,4-cis-diols with flower extracts from Matthiola incana and its role in anthocyanin biosynthesis</atitle><jtitle>Planta</jtitle><addtitle>Planta</addtitle><date>1985-01-01</date><risdate>1985</risdate><volume>165</volume><issue>2</issue><spage>284</spage><epage>287</epage><pages>284-287</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>A cell-free extract from flowers of Matthiola incana catalyzes a NADPH-dependent stereospecific reduction of (+)-dihydrokaempferol to 3,4-cis-leucopelargonidin (5,7,4′-trihydroxyflavan-3,4-cis-diol). The pH-optimum of this reaction is around 6. The rate of reaction with NADH was about 50% of that found with NADPH. (+)-Dihydroquercetin and (+)-dihydromyricetin were also reduced by the enzyme preparation to the corresponding flavan-3,4-cis-diols. Correlation between the genotype of M. incana and the presence of dihydroflavonol 4-reductase is strong evidence that this enzyme is involved in anthocyanin biosynthesis.</abstract><cop>Berlin</cop><pub>Springer-Verlag</pub><pmid>24241054</pmid><doi>10.1007/BF00395052</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0935 |
| ispartof | Planta, 1985-01, Vol.165 (2), p.284-287 |
| issn | 0032-0935 1432-2048 |
| language | eng |
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| subjects | Alleles Analytical, structural and metabolic biochemistry anthocyanins Aromatic and heterocyclic compounds Biological and medical sciences Biosynthesis Chromatography Enzyme activity Enzyme preparations Enzymes Flavonoids flavonols Flowers Fundamental and applied biological sciences. Psychology Genotypes Heterocyclic compounds, pigments Matthiola incana Other biological molecules plant extracts reduction Solvents |
| title | Enzymatic reduction of (+)-dihydroflavonols to flavan-3,4-cis-diols with flower extracts from Matthiola incana and its role in anthocyanin biosynthesis |
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