effects of promoter on transient expression in conifer cell lines
Protoplasts from suspension cultures of somatic embryos of white spruce (Picea glauca Moench Voss) were electroporated with plasmids containing the chimeric genes for chloramphenicol acetyl transferase (CAT) or β-glucuronidase (GUS), under control of one of three promoters. Transient CAT gene expres...
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| Veröffentlicht in: | Theoretical and applied genetics 1990-05, Vol.79 (3), p.353-359 |
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| creator | Bekkaoui, F Datla, R.S.S Pilon, M Tautorus, T.E Crosby, W.L Dunstan, D.I |
| description | Protoplasts from suspension cultures of somatic embryos of white spruce (Picea glauca Moench Voss) were electroporated with plasmids containing the chimeric genes for chloramphenicol acetyl transferase (CAT) or β-glucuronidase (GUS), under control of one of three promoters. Transient CAT gene expression of approximately equal magnitude resulted when the CAT gene was fused to either the cauliflower mosaic virus (CaMV) 35S promoter or the nopaline synthase (NOS) promoter. When the CAT gene was fused to a tandem repeat CaMV 35S promoter (pPBI-363), CAT enzyme activity compared to NOS or 35S promoters increased up to eightfold (cell line WS-34), and were up to 100-fold greater than control (electroporated without plasmid). Comparatively, protoplasts of black spruce (Picea mariana Mill) and jack pine (Pinus banksiana Lamb.), electroporated with pPBI-363, produced increases in CAT activity compared to control of 90-fold and 70-fold, respectively. White spruce (WS-34) protoplasts were subsequently electroporated with the GUS gene fused to the tandem repeat CaMV 35S promoter. Comparatively, GUS enzyme activity increased up to tenfold compared to GUS fused to a CaMV 35S promoter. The results indicated that transient expression of the CAT and GUS genes was influenced by the type of promoter and cell line used, as well as by electroporation conditions. |
| doi_str_mv | 10.1007/bf01186079 |
| format | Article |
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Transient CAT gene expression of approximately equal magnitude resulted when the CAT gene was fused to either the cauliflower mosaic virus (CaMV) 35S promoter or the nopaline synthase (NOS) promoter. When the CAT gene was fused to a tandem repeat CaMV 35S promoter (pPBI-363), CAT enzyme activity compared to NOS or 35S promoters increased up to eightfold (cell line WS-34), and were up to 100-fold greater than control (electroporated without plasmid). Comparatively, protoplasts of black spruce (Picea mariana Mill) and jack pine (Pinus banksiana Lamb.), electroporated with pPBI-363, produced increases in CAT activity compared to control of 90-fold and 70-fold, respectively. White spruce (WS-34) protoplasts were subsequently electroporated with the GUS gene fused to the tandem repeat CaMV 35S promoter. Comparatively, GUS enzyme activity increased up to tenfold compared to GUS fused to a CaMV 35S promoter. The results indicated that transient expression of the CAT and GUS genes was influenced by the type of promoter and cell line used, as well as by electroporation conditions.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/bf01186079</identifier><identifier>PMID: 24226354</identifier><identifier>CODEN: THAGA6</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Agronomy. Soil science and plant productions ; beta-glucuronidase ; Biological and medical sciences ; Biotechnology ; Cauliflower mosaic virus ; chloramphenicol acetyltransferase ; electroporation ; enzyme activity ; Fundamental and applied biological sciences. Psychology ; gene expression ; Genetic engineering ; Genetic engineering applications ; genetic regulation ; Genetic technics ; genetic transformation ; Genetics and breeding of economic plants ; genotype ; Methods. Procedures. Technologies ; Picea glauca ; Picea mariana ; Pinus banksiana ; Plant breeding: fundamental aspects and methodology ; plasmids ; promoter regions ; protoplasts ; reporter genes ; somatic embryogenesis</subject><ispartof>Theoretical and applied genetics, 1990-05, Vol.79 (3), p.353-359</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-b5771fcdf8efdaddc2d91985c0923f042054e5c2e583b40b0de628022a59be093</citedby><cites>FETCH-LOGICAL-c404t-b5771fcdf8efdaddc2d91985c0923f042054e5c2e583b40b0de628022a59be093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6870051$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24226354$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bekkaoui, F</creatorcontrib><creatorcontrib>Datla, R.S.S</creatorcontrib><creatorcontrib>Pilon, M</creatorcontrib><creatorcontrib>Tautorus, T.E</creatorcontrib><creatorcontrib>Crosby, W.L</creatorcontrib><creatorcontrib>Dunstan, D.I</creatorcontrib><title>effects of promoter on transient expression in conifer cell lines</title><title>Theoretical and applied genetics</title><addtitle>Theor Appl Genet</addtitle><description>Protoplasts from suspension cultures of somatic embryos of white spruce (Picea glauca Moench Voss) were electroporated with plasmids containing the chimeric genes for chloramphenicol acetyl transferase (CAT) or β-glucuronidase (GUS), under control of one of three promoters. Transient CAT gene expression of approximately equal magnitude resulted when the CAT gene was fused to either the cauliflower mosaic virus (CaMV) 35S promoter or the nopaline synthase (NOS) promoter. When the CAT gene was fused to a tandem repeat CaMV 35S promoter (pPBI-363), CAT enzyme activity compared to NOS or 35S promoters increased up to eightfold (cell line WS-34), and were up to 100-fold greater than control (electroporated without plasmid). Comparatively, protoplasts of black spruce (Picea mariana Mill) and jack pine (Pinus banksiana Lamb.), electroporated with pPBI-363, produced increases in CAT activity compared to control of 90-fold and 70-fold, respectively. White spruce (WS-34) protoplasts were subsequently electroporated with the GUS gene fused to the tandem repeat CaMV 35S promoter. Comparatively, GUS enzyme activity increased up to tenfold compared to GUS fused to a CaMV 35S promoter. The results indicated that transient expression of the CAT and GUS genes was influenced by the type of promoter and cell line used, as well as by electroporation conditions.</description><subject>Agronomy. Soil science and plant productions</subject><subject>beta-glucuronidase</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cauliflower mosaic virus</subject><subject>chloramphenicol acetyltransferase</subject><subject>electroporation</subject><subject>enzyme activity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>Genetic engineering</subject><subject>Genetic engineering applications</subject><subject>genetic regulation</subject><subject>Genetic technics</subject><subject>genetic transformation</subject><subject>Genetics and breeding of economic plants</subject><subject>genotype</subject><subject>Methods. Procedures. Technologies</subject><subject>Picea glauca</subject><subject>Picea mariana</subject><subject>Pinus banksiana</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>plasmids</subject><subject>promoter regions</subject><subject>protoplasts</subject><subject>reporter genes</subject><subject>somatic embryogenesis</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNo90E1LAzEQBuAgiq3Viz9A9-BBhNXJbLIfx1qsCgUP2vOSzU4kst3UZAv6701p62lg5mGYeRm75HDPAYqHxgDnZQ5FdcTGXGSYIgo8ZmMAAaksJI7YWQhfAIASslM2imPMMynGbErGkB5C4kyy9m7lBvKJ65PBqz5Y6oeEftaeQrCxaftEu96aSDR1XdLZnsI5OzGqC3SxrxO2nD99zF7Sxdvz62y6SLUAMaSNLApudGtKMq1qW41txatSaqgwMyAQpCCpkWSZNQIaaCnHEhCVrBqCKpuw293eeOb3hsJQr2zYnqF6cptQcyErnseXMdK7HdXeheDJ1GtvV8r_1hzqbWT14_wQWcRX-72bZkXtPz1kFMHNHqigVWdiMtqGf5eXBYDkkV3vmFGuVp8-kuU7As8AC8gBq-wPvQp7LQ</recordid><startdate>199005</startdate><enddate>199005</enddate><creator>Bekkaoui, F</creator><creator>Datla, R.S.S</creator><creator>Pilon, M</creator><creator>Tautorus, T.E</creator><creator>Crosby, W.L</creator><creator>Dunstan, D.I</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199005</creationdate><title>effects of promoter on transient expression in conifer cell lines</title><author>Bekkaoui, F ; Datla, R.S.S ; Pilon, M ; Tautorus, T.E ; Crosby, W.L ; Dunstan, D.I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-b5771fcdf8efdaddc2d91985c0923f042054e5c2e583b40b0de628022a59be093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>beta-glucuronidase</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cauliflower mosaic virus</topic><topic>chloramphenicol acetyltransferase</topic><topic>electroporation</topic><topic>enzyme activity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>Genetic engineering</topic><topic>Genetic engineering applications</topic><topic>genetic regulation</topic><topic>Genetic technics</topic><topic>genetic transformation</topic><topic>Genetics and breeding of economic plants</topic><topic>genotype</topic><topic>Methods. Procedures. Technologies</topic><topic>Picea glauca</topic><topic>Picea mariana</topic><topic>Pinus banksiana</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>plasmids</topic><topic>promoter regions</topic><topic>protoplasts</topic><topic>reporter genes</topic><topic>somatic embryogenesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bekkaoui, F</creatorcontrib><creatorcontrib>Datla, R.S.S</creatorcontrib><creatorcontrib>Pilon, M</creatorcontrib><creatorcontrib>Tautorus, T.E</creatorcontrib><creatorcontrib>Crosby, W.L</creatorcontrib><creatorcontrib>Dunstan, D.I</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theoretical and applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bekkaoui, F</au><au>Datla, R.S.S</au><au>Pilon, M</au><au>Tautorus, T.E</au><au>Crosby, W.L</au><au>Dunstan, D.I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>effects of promoter on transient expression in conifer cell lines</atitle><jtitle>Theoretical and applied genetics</jtitle><addtitle>Theor Appl Genet</addtitle><date>1990-05</date><risdate>1990</risdate><volume>79</volume><issue>3</issue><spage>353</spage><epage>359</epage><pages>353-359</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><coden>THAGA6</coden><abstract>Protoplasts from suspension cultures of somatic embryos of white spruce (Picea glauca Moench Voss) were electroporated with plasmids containing the chimeric genes for chloramphenicol acetyl transferase (CAT) or β-glucuronidase (GUS), under control of one of three promoters. Transient CAT gene expression of approximately equal magnitude resulted when the CAT gene was fused to either the cauliflower mosaic virus (CaMV) 35S promoter or the nopaline synthase (NOS) promoter. When the CAT gene was fused to a tandem repeat CaMV 35S promoter (pPBI-363), CAT enzyme activity compared to NOS or 35S promoters increased up to eightfold (cell line WS-34), and were up to 100-fold greater than control (electroporated without plasmid). Comparatively, protoplasts of black spruce (Picea mariana Mill) and jack pine (Pinus banksiana Lamb.), electroporated with pPBI-363, produced increases in CAT activity compared to control of 90-fold and 70-fold, respectively. White spruce (WS-34) protoplasts were subsequently electroporated with the GUS gene fused to the tandem repeat CaMV 35S promoter. Comparatively, GUS enzyme activity increased up to tenfold compared to GUS fused to a CaMV 35S promoter. The results indicated that transient expression of the CAT and GUS genes was influenced by the type of promoter and cell line used, as well as by electroporation conditions.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>24226354</pmid><doi>10.1007/bf01186079</doi><tpages>7</tpages></addata></record> |
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| ispartof | Theoretical and applied genetics, 1990-05, Vol.79 (3), p.353-359 |
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| subjects | Agronomy. Soil science and plant productions beta-glucuronidase Biological and medical sciences Biotechnology Cauliflower mosaic virus chloramphenicol acetyltransferase electroporation enzyme activity Fundamental and applied biological sciences. Psychology gene expression Genetic engineering Genetic engineering applications genetic regulation Genetic technics genetic transformation Genetics and breeding of economic plants genotype Methods. Procedures. Technologies Picea glauca Picea mariana Pinus banksiana Plant breeding: fundamental aspects and methodology plasmids promoter regions protoplasts reporter genes somatic embryogenesis |
| title | effects of promoter on transient expression in conifer cell lines |
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