Cellulysin induces downy mildew disease resistance in pearl millet driven through defense response
The responses to cellulysin as an immune inducer in pearl millet that confers downy mildew resistance mediated through lipoxygenase (LOX), a jasmonate-dependent enzyme involved in defence signalling, are discussed in this paper. The susceptible pearl millet cultivar 7042S was treated with cellulysin...
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| Veröffentlicht in: | European journal of plant pathology 2013-12, Vol.137 (4), p.707-717 |
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| creator | Pushpalatha, H. G Sudisha, J Shetty, H. Shekar |
| description | The responses to cellulysin as an immune inducer in pearl millet that confers downy mildew resistance mediated through lipoxygenase (LOX), a jasmonate-dependent enzyme involved in defence signalling, are discussed in this paper. The susceptible pearl millet cultivar 7042S was treated with cellulysin at 10, 15, 20, 30 and 50 μg/ml concentrations. All tested concentrations showed enhanced seed germination and seedling vigour when compared with the untreated control. Maximum seed germination of 92 % and seedling vigour was obtained following 20 μg/ml cellulysin treatment. Significant (P |
| doi_str_mv | 10.1007/s10658-013-0281-9 |
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G ; Sudisha, J ; Shetty, H. Shekar</creator><creatorcontrib>Pushpalatha, H. G ; Sudisha, J ; Shetty, H. Shekar</creatorcontrib><description>The responses to cellulysin as an immune inducer in pearl millet that confers downy mildew resistance mediated through lipoxygenase (LOX), a jasmonate-dependent enzyme involved in defence signalling, are discussed in this paper. The susceptible pearl millet cultivar 7042S was treated with cellulysin at 10, 15, 20, 30 and 50 μg/ml concentrations. All tested concentrations showed enhanced seed germination and seedling vigour when compared with the untreated control. Maximum seed germination of 92 % and seedling vigour was obtained following 20 μg/ml cellulysin treatment. Significant (P < 0.05) downy mildew disease protection of 67 % and 71 % was observed when cellulysin was used at 20 μg/ml under greenhouse and field conditions, respectively. Further studies showed that the resistance induced by cellulysin treatment in pearl millet plant was systemic, required a minimum of 4 days to achieve maximum resistance development after pathogen inoculation seedling inoculation (five-day-old), and was sustained throughout the plant’s life. Plants raised from cellulysin-treated seeds and challenge inoculated at tillering (25-day-old) and inflorescence (45-day-old) showed persistence in resistance till the end of the crop period. A notable increase in LOX activity was observed in all the tested concentrations of cellulysin in plants inoculated with the pathogen at 24 h, compared to the control. However, a maximum 6-fold increase in LOX activity was noticed using a cellulysin concentration of 20 μg/ml 48 hours post inoculation. In contrast, glucanase (GLU) activity was high in control inoculated seedlings, but was low in cellulysin treated samples at all time intervals. The optimal cellulysin treatment (20 μg/ml) provided enhanced vegetative and reproductive parameters that resulted in higher yield compared to the untreated control.</description><identifier>ISSN: 0929-1873</identifier><identifier>EISSN: 1573-8469</identifier><identifier>DOI: 10.1007/s10658-013-0281-9</identifier><language>eng</language><publisher>Dordrecht: Springer-Verlag</publisher><subject>Agriculture ; Airborne microorganisms ; Biomedical and Life Sciences ; Cellulase ; Cultivars ; Disease resistance ; downy mildew ; Ecology ; Grain ; greenhouses ; induced resistance ; Life Sciences ; lipoxygenase ; Millet ; Original Research ; Pathogens ; Pennisetum glaucum ; Plant diseases ; Plant Pathology ; Plant resistance ; Plant Sciences ; Seed germination ; Seedlings ; seeds ; tillering ; vigor</subject><ispartof>European journal of plant pathology, 2013-12, Vol.137 (4), p.707-717</ispartof><rights>KNPV 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-fa6f936497a280db71d03ee4e6c66520e08165278e85dddb06b20cf9d9cd59843</citedby><cites>FETCH-LOGICAL-c373t-fa6f936497a280db71d03ee4e6c66520e08165278e85dddb06b20cf9d9cd59843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10658-013-0281-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10658-013-0281-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Pushpalatha, H. G</creatorcontrib><creatorcontrib>Sudisha, J</creatorcontrib><creatorcontrib>Shetty, H. Shekar</creatorcontrib><title>Cellulysin induces downy mildew disease resistance in pearl millet driven through defense response</title><title>European journal of plant pathology</title><addtitle>Eur J Plant Pathol</addtitle><description>The responses to cellulysin as an immune inducer in pearl millet that confers downy mildew resistance mediated through lipoxygenase (LOX), a jasmonate-dependent enzyme involved in defence signalling, are discussed in this paper. The susceptible pearl millet cultivar 7042S was treated with cellulysin at 10, 15, 20, 30 and 50 μg/ml concentrations. All tested concentrations showed enhanced seed germination and seedling vigour when compared with the untreated control. Maximum seed germination of 92 % and seedling vigour was obtained following 20 μg/ml cellulysin treatment. Significant (P < 0.05) downy mildew disease protection of 67 % and 71 % was observed when cellulysin was used at 20 μg/ml under greenhouse and field conditions, respectively. Further studies showed that the resistance induced by cellulysin treatment in pearl millet plant was systemic, required a minimum of 4 days to achieve maximum resistance development after pathogen inoculation seedling inoculation (five-day-old), and was sustained throughout the plant’s life. Plants raised from cellulysin-treated seeds and challenge inoculated at tillering (25-day-old) and inflorescence (45-day-old) showed persistence in resistance till the end of the crop period. A notable increase in LOX activity was observed in all the tested concentrations of cellulysin in plants inoculated with the pathogen at 24 h, compared to the control. However, a maximum 6-fold increase in LOX activity was noticed using a cellulysin concentration of 20 μg/ml 48 hours post inoculation. In contrast, glucanase (GLU) activity was high in control inoculated seedlings, but was low in cellulysin treated samples at all time intervals. The optimal cellulysin treatment (20 μg/ml) provided enhanced vegetative and reproductive parameters that resulted in higher yield compared to the untreated control.</description><subject>Agriculture</subject><subject>Airborne microorganisms</subject><subject>Biomedical and Life Sciences</subject><subject>Cellulase</subject><subject>Cultivars</subject><subject>Disease resistance</subject><subject>downy mildew</subject><subject>Ecology</subject><subject>Grain</subject><subject>greenhouses</subject><subject>induced resistance</subject><subject>Life Sciences</subject><subject>lipoxygenase</subject><subject>Millet</subject><subject>Original Research</subject><subject>Pathogens</subject><subject>Pennisetum glaucum</subject><subject>Plant diseases</subject><subject>Plant Pathology</subject><subject>Plant resistance</subject><subject>Plant Sciences</subject><subject>Seed germination</subject><subject>Seedlings</subject><subject>seeds</subject><subject>tillering</subject><subject>vigor</subject><issn>0929-1873</issn><issn>1573-8469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kTtvFDEURi2USGwSfgAVlmhoJly_7RKtCCBFooDUlnd8Z-No1rPYM0T77_FqKBBFquvinPv4TMhbBrcMwHysDLSyHTDRAbesc6_IhikjOiu1uyAbcNx1zBrxmlzV-gTNcY5vyG6L47iMp5oyTTkuPVYap-d8ooc0RnymMVUMFWnBmuocco-No0cMZTwjI840lvQbM50fy7TsH2nEAfNqHKf2uCGXQxgrvvlbr8nD3eef26_d_fcv37af7rteGDF3Q9CDE1o6E7iFuDMsgkCUqHutFQcEy1o1Fq2KMe5A7zj0g4uuj8pZKa7Jh7XvsUy_FqyzP6Tat_NCxmmpnkllVRulREPf_4c-TUvJbbtGSWO0kPJMsZXqy1RrwcEfSzqEcvIM_Dl1v6buW-r-nLp3zeGrUxub91j-6fyC9G6VhjD5sC-p-ocfHJhs32RBaS7-AAjAjsM</recordid><startdate>20131201</startdate><enddate>20131201</enddate><creator>Pushpalatha, H. 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G ; Sudisha, J ; Shetty, H. Shekar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-fa6f936497a280db71d03ee4e6c66520e08165278e85dddb06b20cf9d9cd59843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Agriculture</topic><topic>Airborne microorganisms</topic><topic>Biomedical and Life Sciences</topic><topic>Cellulase</topic><topic>Cultivars</topic><topic>Disease resistance</topic><topic>downy mildew</topic><topic>Ecology</topic><topic>Grain</topic><topic>greenhouses</topic><topic>induced resistance</topic><topic>Life Sciences</topic><topic>lipoxygenase</topic><topic>Millet</topic><topic>Original Research</topic><topic>Pathogens</topic><topic>Pennisetum glaucum</topic><topic>Plant diseases</topic><topic>Plant Pathology</topic><topic>Plant resistance</topic><topic>Plant Sciences</topic><topic>Seed germination</topic><topic>Seedlings</topic><topic>seeds</topic><topic>tillering</topic><topic>vigor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pushpalatha, H. 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G</au><au>Sudisha, J</au><au>Shetty, H. Shekar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cellulysin induces downy mildew disease resistance in pearl millet driven through defense response</atitle><jtitle>European journal of plant pathology</jtitle><stitle>Eur J Plant Pathol</stitle><date>2013-12-01</date><risdate>2013</risdate><volume>137</volume><issue>4</issue><spage>707</spage><epage>717</epage><pages>707-717</pages><issn>0929-1873</issn><eissn>1573-8469</eissn><abstract>The responses to cellulysin as an immune inducer in pearl millet that confers downy mildew resistance mediated through lipoxygenase (LOX), a jasmonate-dependent enzyme involved in defence signalling, are discussed in this paper. The susceptible pearl millet cultivar 7042S was treated with cellulysin at 10, 15, 20, 30 and 50 μg/ml concentrations. All tested concentrations showed enhanced seed germination and seedling vigour when compared with the untreated control. Maximum seed germination of 92 % and seedling vigour was obtained following 20 μg/ml cellulysin treatment. Significant (P < 0.05) downy mildew disease protection of 67 % and 71 % was observed when cellulysin was used at 20 μg/ml under greenhouse and field conditions, respectively. Further studies showed that the resistance induced by cellulysin treatment in pearl millet plant was systemic, required a minimum of 4 days to achieve maximum resistance development after pathogen inoculation seedling inoculation (five-day-old), and was sustained throughout the plant’s life. Plants raised from cellulysin-treated seeds and challenge inoculated at tillering (25-day-old) and inflorescence (45-day-old) showed persistence in resistance till the end of the crop period. A notable increase in LOX activity was observed in all the tested concentrations of cellulysin in plants inoculated with the pathogen at 24 h, compared to the control. However, a maximum 6-fold increase in LOX activity was noticed using a cellulysin concentration of 20 μg/ml 48 hours post inoculation. In contrast, glucanase (GLU) activity was high in control inoculated seedlings, but was low in cellulysin treated samples at all time intervals. The optimal cellulysin treatment (20 μg/ml) provided enhanced vegetative and reproductive parameters that resulted in higher yield compared to the untreated control.</abstract><cop>Dordrecht</cop><pub>Springer-Verlag</pub><doi>10.1007/s10658-013-0281-9</doi><tpages>11</tpages></addata></record> |
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| subjects | Agriculture Airborne microorganisms Biomedical and Life Sciences Cellulase Cultivars Disease resistance downy mildew Ecology Grain greenhouses induced resistance Life Sciences lipoxygenase Millet Original Research Pathogens Pennisetum glaucum Plant diseases Plant Pathology Plant resistance Plant Sciences Seed germination Seedlings seeds tillering vigor |
| title | Cellulysin induces downy mildew disease resistance in pearl millet driven through defense response |
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