Effect of PEGylation on Biodistribution and Gene Silencing of siRNA/Lipid Nanoparticle Complexes
ABSTRACT Purpose To determine the influence of physicochemical properties of lipid nanoparticles (LNPs) carrying siRNA on their gene silencing in vivo . Mechanistic understanding of how the architecture of the nanoparticle can alter gene expression has also been studied. Methods The effect of 3-N-[(...
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| Veröffentlicht in: | Pharmaceutical research 2013-02, Vol.30 (2), p.342-351 |
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| creator | Bao, Yanjie Jin, Yi Chivukula, Padmanabh Zhang, Jun Liu, Yun Liu, Jian Clamme, Jean-Pierre Mahato, Ram I. Ng, Dominic Ying, Wenbin Wang, Yiting Yu, Lei |
| description | ABSTRACT
Purpose
To determine the influence of physicochemical properties of lipid nanoparticles (LNPs) carrying siRNA on their gene silencing
in vivo
. Mechanistic understanding of how the architecture of the nanoparticle can alter gene expression has also been studied.
Methods
The effect of 3-N-[(ω-methoxypoly(ethylene glycol)2000)carbamoyl]-1,2-dimyristyloxy-propylamine (PEG-C-DMA) on hepatic distribution and FVII gene silencing was determined. FVII mRNA in hepatocytes and liver tissues was determined by Q-PCR. Hepatic distribution was quantified by FACS analysis using Cy5 labeled siRNA.
Results
Gene silencing was highly dependent on the amount of PEG-C-DMA present. FVII gene silencing inversely correlated to the amount of PEG-C-DMA in LNPs. High FVII gene silencing was obtained
in vitro
and
in vivo
when the molar ratio of PEG-C-DMA to lipid was 0.5 mol%. Surprisingly, PEGylation didn’t alter the hepatic distribution of the LNPs at 5 h post administration. Instead the amount of PEG present in the LNPs has an effect on red blood cell disruption at low pH.
Conclusion
Low but sufficient PEG-C-DMA amount in LNPs plays an important role for efficient FVII gene silencing
in vivo
. PEGylation did not alter the hepatic distribution of LNPs, but altered gene silencing efficacy by potentially reducing endosomal disruption. |
| doi_str_mv | 10.1007/s11095-012-0874-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1458529216</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2873237291</sourcerecordid><originalsourceid>FETCH-LOGICAL-c501t-37dd7e4a6e567dc0b773fa762889ea5647a7e0c1b4bfa19e90df547214788713</originalsourceid><addsrcrecordid>eNp1kE2LFDEQhoMo7rj6A7xIgwhe2k3SSapz3B3GURhW0T14i-l0ZcnSk26TbnD_vRln_EAQCgJVT70pHkKeM_qGUQoXmTGqZU0Zr2kLolYPyIpJaGpNxZeHZEWBi7oM2Bl5kvMdpbRlWjwmZ5zrtlFCrMjXjffo5mr01cfN9n6wcxhjVeoqjH3Icwrd8rNlY19tMWL1OQwYXYi3h50cPl1fXuzCFPrq2sZxsmkObsBqPe6nAb9jfkoeeTtkfHZ6z8nN283N-l29-7B9v77c1U5SNtcN9D2gsAqlgt7RDqDxFhRvW41WKgEWkDrWic5bplHT3ksBnAloW2DNOXl9jJ3S-G3BPJt9yA6HwUYcl2yYkK3kmjNV0Jf_oHfjkmI5zjAOnCtJm6ZQ7Ei5NOac0Jsphb1N94ZRc7BvjvZNsW8O9s0h-cUpeen22P_e-KW7AK9OgM3ODj7ZYjL_4ZQG2WgoHD9yuYziLaa_Tvzv7z8Ay0SbHg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1272265033</pqid></control><display><type>article</type><title>Effect of PEGylation on Biodistribution and Gene Silencing of siRNA/Lipid Nanoparticle Complexes</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Bao, Yanjie ; Jin, Yi ; Chivukula, Padmanabh ; Zhang, Jun ; Liu, Yun ; Liu, Jian ; Clamme, Jean-Pierre ; Mahato, Ram I. ; Ng, Dominic ; Ying, Wenbin ; Wang, Yiting ; Yu, Lei</creator><creatorcontrib>Bao, Yanjie ; Jin, Yi ; Chivukula, Padmanabh ; Zhang, Jun ; Liu, Yun ; Liu, Jian ; Clamme, Jean-Pierre ; Mahato, Ram I. ; Ng, Dominic ; Ying, Wenbin ; Wang, Yiting ; Yu, Lei</creatorcontrib><description>ABSTRACT
Purpose
To determine the influence of physicochemical properties of lipid nanoparticles (LNPs) carrying siRNA on their gene silencing
in vivo
. Mechanistic understanding of how the architecture of the nanoparticle can alter gene expression has also been studied.
Methods
The effect of 3-N-[(ω-methoxypoly(ethylene glycol)2000)carbamoyl]-1,2-dimyristyloxy-propylamine (PEG-C-DMA) on hepatic distribution and FVII gene silencing was determined. FVII mRNA in hepatocytes and liver tissues was determined by Q-PCR. Hepatic distribution was quantified by FACS analysis using Cy5 labeled siRNA.
Results
Gene silencing was highly dependent on the amount of PEG-C-DMA present. FVII gene silencing inversely correlated to the amount of PEG-C-DMA in LNPs. High FVII gene silencing was obtained
in vitro
and
in vivo
when the molar ratio of PEG-C-DMA to lipid was 0.5 mol%. Surprisingly, PEGylation didn’t alter the hepatic distribution of the LNPs at 5 h post administration. Instead the amount of PEG present in the LNPs has an effect on red blood cell disruption at low pH.
Conclusion
Low but sufficient PEG-C-DMA amount in LNPs plays an important role for efficient FVII gene silencing
in vivo
. PEGylation did not alter the hepatic distribution of LNPs, but altered gene silencing efficacy by potentially reducing endosomal disruption.</description><identifier>ISSN: 0724-8741</identifier><identifier>EISSN: 1573-904X</identifier><identifier>DOI: 10.1007/s11095-012-0874-6</identifier><identifier>PMID: 22983644</identifier><identifier>CODEN: PHREEB</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Animals ; Biochemistry ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedical Engineering and Bioengineering ; Biomedicine ; Cells, Cultured ; Drug delivery systems ; Factor VII - genetics ; Gene therapy ; General pharmacology ; Hepatocytes - metabolism ; Lipids ; Lipids - chemistry ; Liver - metabolism ; Male ; Medical Law ; Medical sciences ; Mice ; Mice, Inbred C57BL ; Nanoparticles ; Nanoparticles - chemistry ; Pharmaceutical sciences ; Pharmaceutical technology. Pharmaceutical industry ; Pharmacology. Drug treatments ; Pharmacology/Toxicology ; Pharmacy ; Polyethylene glycol ; Polyethylene Glycols - chemistry ; Propylamines - chemistry ; Rats ; Rats, Sprague-Dawley ; Research Paper ; Ribonucleic acid ; RNA ; RNA Interference ; RNA, Messenger - genetics ; RNA, Small Interfering - administration & dosage ; RNA, Small Interfering - genetics ; RNA, Small Interfering - pharmacokinetics</subject><ispartof>Pharmaceutical research, 2013-02, Vol.30 (2), p.342-351</ispartof><rights>Springer Science+Business Media, LLC 2012</rights><rights>2014 INIST-CNRS</rights><rights>Springer Science+Business Media New York 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c501t-37dd7e4a6e567dc0b773fa762889ea5647a7e0c1b4bfa19e90df547214788713</citedby><cites>FETCH-LOGICAL-c501t-37dd7e4a6e567dc0b773fa762889ea5647a7e0c1b4bfa19e90df547214788713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11095-012-0874-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11095-012-0874-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26975397$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22983644$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bao, Yanjie</creatorcontrib><creatorcontrib>Jin, Yi</creatorcontrib><creatorcontrib>Chivukula, Padmanabh</creatorcontrib><creatorcontrib>Zhang, Jun</creatorcontrib><creatorcontrib>Liu, Yun</creatorcontrib><creatorcontrib>Liu, Jian</creatorcontrib><creatorcontrib>Clamme, Jean-Pierre</creatorcontrib><creatorcontrib>Mahato, Ram I.</creatorcontrib><creatorcontrib>Ng, Dominic</creatorcontrib><creatorcontrib>Ying, Wenbin</creatorcontrib><creatorcontrib>Wang, Yiting</creatorcontrib><creatorcontrib>Yu, Lei</creatorcontrib><title>Effect of PEGylation on Biodistribution and Gene Silencing of siRNA/Lipid Nanoparticle Complexes</title><title>Pharmaceutical research</title><addtitle>Pharm Res</addtitle><addtitle>Pharm Res</addtitle><description>ABSTRACT
Purpose
To determine the influence of physicochemical properties of lipid nanoparticles (LNPs) carrying siRNA on their gene silencing
in vivo
. Mechanistic understanding of how the architecture of the nanoparticle can alter gene expression has also been studied.
Methods
The effect of 3-N-[(ω-methoxypoly(ethylene glycol)2000)carbamoyl]-1,2-dimyristyloxy-propylamine (PEG-C-DMA) on hepatic distribution and FVII gene silencing was determined. FVII mRNA in hepatocytes and liver tissues was determined by Q-PCR. Hepatic distribution was quantified by FACS analysis using Cy5 labeled siRNA.
Results
Gene silencing was highly dependent on the amount of PEG-C-DMA present. FVII gene silencing inversely correlated to the amount of PEG-C-DMA in LNPs. High FVII gene silencing was obtained
in vitro
and
in vivo
when the molar ratio of PEG-C-DMA to lipid was 0.5 mol%. Surprisingly, PEGylation didn’t alter the hepatic distribution of the LNPs at 5 h post administration. Instead the amount of PEG present in the LNPs has an effect on red blood cell disruption at low pH.
Conclusion
Low but sufficient PEG-C-DMA amount in LNPs plays an important role for efficient FVII gene silencing
in vivo
. PEGylation did not alter the hepatic distribution of LNPs, but altered gene silencing efficacy by potentially reducing endosomal disruption.</description><subject>Animals</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedicine</subject><subject>Cells, Cultured</subject><subject>Drug delivery systems</subject><subject>Factor VII - genetics</subject><subject>Gene therapy</subject><subject>General pharmacology</subject><subject>Hepatocytes - metabolism</subject><subject>Lipids</subject><subject>Lipids - chemistry</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Medical Law</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Pharmaceutical sciences</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. Drug treatments</subject><subject>Pharmacology/Toxicology</subject><subject>Pharmacy</subject><subject>Polyethylene glycol</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Propylamines - chemistry</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Research Paper</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Interference</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Small Interfering - administration & dosage</subject><subject>RNA, Small Interfering - genetics</subject><subject>RNA, Small Interfering - pharmacokinetics</subject><issn>0724-8741</issn><issn>1573-904X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kE2LFDEQhoMo7rj6A7xIgwhe2k3SSapz3B3GURhW0T14i-l0ZcnSk26TbnD_vRln_EAQCgJVT70pHkKeM_qGUQoXmTGqZU0Zr2kLolYPyIpJaGpNxZeHZEWBi7oM2Bl5kvMdpbRlWjwmZ5zrtlFCrMjXjffo5mr01cfN9n6wcxhjVeoqjH3Icwrd8rNlY19tMWL1OQwYXYi3h50cPl1fXuzCFPrq2sZxsmkObsBqPe6nAb9jfkoeeTtkfHZ6z8nN283N-l29-7B9v77c1U5SNtcN9D2gsAqlgt7RDqDxFhRvW41WKgEWkDrWic5bplHT3ksBnAloW2DNOXl9jJ3S-G3BPJt9yA6HwUYcl2yYkK3kmjNV0Jf_oHfjkmI5zjAOnCtJm6ZQ7Ei5NOac0Jsphb1N94ZRc7BvjvZNsW8O9s0h-cUpeen22P_e-KW7AK9OgM3ODj7ZYjL_4ZQG2WgoHD9yuYziLaa_Tvzv7z8Ay0SbHg</recordid><startdate>20130201</startdate><enddate>20130201</enddate><creator>Bao, Yanjie</creator><creator>Jin, Yi</creator><creator>Chivukula, Padmanabh</creator><creator>Zhang, Jun</creator><creator>Liu, Yun</creator><creator>Liu, Jian</creator><creator>Clamme, Jean-Pierre</creator><creator>Mahato, Ram I.</creator><creator>Ng, Dominic</creator><creator>Ying, Wenbin</creator><creator>Wang, Yiting</creator><creator>Yu, Lei</creator><general>Springer US</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20130201</creationdate><title>Effect of PEGylation on Biodistribution and Gene Silencing of siRNA/Lipid Nanoparticle Complexes</title><author>Bao, Yanjie ; Jin, Yi ; Chivukula, Padmanabh ; Zhang, Jun ; Liu, Yun ; Liu, Jian ; Clamme, Jean-Pierre ; Mahato, Ram I. ; Ng, Dominic ; Ying, Wenbin ; Wang, Yiting ; Yu, Lei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c501t-37dd7e4a6e567dc0b773fa762889ea5647a7e0c1b4bfa19e90df547214788713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Biomedicine</topic><topic>Cells, Cultured</topic><topic>Drug delivery systems</topic><topic>Factor VII - genetics</topic><topic>Gene therapy</topic><topic>General pharmacology</topic><topic>Hepatocytes - metabolism</topic><topic>Lipids</topic><topic>Lipids - chemistry</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Medical Law</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Nanoparticles</topic><topic>Nanoparticles - chemistry</topic><topic>Pharmaceutical sciences</topic><topic>Pharmaceutical technology. Pharmaceutical industry</topic><topic>Pharmacology. Drug treatments</topic><topic>Pharmacology/Toxicology</topic><topic>Pharmacy</topic><topic>Polyethylene glycol</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Propylamines - chemistry</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Research Paper</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA Interference</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Small Interfering - administration & dosage</topic><topic>RNA, Small Interfering - genetics</topic><topic>RNA, Small Interfering - pharmacokinetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bao, Yanjie</creatorcontrib><creatorcontrib>Jin, Yi</creatorcontrib><creatorcontrib>Chivukula, Padmanabh</creatorcontrib><creatorcontrib>Zhang, Jun</creatorcontrib><creatorcontrib>Liu, Yun</creatorcontrib><creatorcontrib>Liu, Jian</creatorcontrib><creatorcontrib>Clamme, Jean-Pierre</creatorcontrib><creatorcontrib>Mahato, Ram I.</creatorcontrib><creatorcontrib>Ng, Dominic</creatorcontrib><creatorcontrib>Ying, Wenbin</creatorcontrib><creatorcontrib>Wang, Yiting</creatorcontrib><creatorcontrib>Yu, Lei</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Pharmaceutical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bao, Yanjie</au><au>Jin, Yi</au><au>Chivukula, Padmanabh</au><au>Zhang, Jun</au><au>Liu, Yun</au><au>Liu, Jian</au><au>Clamme, Jean-Pierre</au><au>Mahato, Ram I.</au><au>Ng, Dominic</au><au>Ying, Wenbin</au><au>Wang, Yiting</au><au>Yu, Lei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of PEGylation on Biodistribution and Gene Silencing of siRNA/Lipid Nanoparticle Complexes</atitle><jtitle>Pharmaceutical research</jtitle><stitle>Pharm Res</stitle><addtitle>Pharm Res</addtitle><date>2013-02-01</date><risdate>2013</risdate><volume>30</volume><issue>2</issue><spage>342</spage><epage>351</epage><pages>342-351</pages><issn>0724-8741</issn><eissn>1573-904X</eissn><coden>PHREEB</coden><abstract>ABSTRACT
Purpose
To determine the influence of physicochemical properties of lipid nanoparticles (LNPs) carrying siRNA on their gene silencing
in vivo
. Mechanistic understanding of how the architecture of the nanoparticle can alter gene expression has also been studied.
Methods
The effect of 3-N-[(ω-methoxypoly(ethylene glycol)2000)carbamoyl]-1,2-dimyristyloxy-propylamine (PEG-C-DMA) on hepatic distribution and FVII gene silencing was determined. FVII mRNA in hepatocytes and liver tissues was determined by Q-PCR. Hepatic distribution was quantified by FACS analysis using Cy5 labeled siRNA.
Results
Gene silencing was highly dependent on the amount of PEG-C-DMA present. FVII gene silencing inversely correlated to the amount of PEG-C-DMA in LNPs. High FVII gene silencing was obtained
in vitro
and
in vivo
when the molar ratio of PEG-C-DMA to lipid was 0.5 mol%. Surprisingly, PEGylation didn’t alter the hepatic distribution of the LNPs at 5 h post administration. Instead the amount of PEG present in the LNPs has an effect on red blood cell disruption at low pH.
Conclusion
Low but sufficient PEG-C-DMA amount in LNPs plays an important role for efficient FVII gene silencing
in vivo
. PEGylation did not alter the hepatic distribution of LNPs, but altered gene silencing efficacy by potentially reducing endosomal disruption.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>22983644</pmid><doi>10.1007/s11095-012-0874-6</doi><tpages>10</tpages></addata></record> |
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| ispartof | Pharmaceutical research, 2013-02, Vol.30 (2), p.342-351 |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animals Biochemistry Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering and Bioengineering Biomedicine Cells, Cultured Drug delivery systems Factor VII - genetics Gene therapy General pharmacology Hepatocytes - metabolism Lipids Lipids - chemistry Liver - metabolism Male Medical Law Medical sciences Mice Mice, Inbred C57BL Nanoparticles Nanoparticles - chemistry Pharmaceutical sciences Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Pharmacology/Toxicology Pharmacy Polyethylene glycol Polyethylene Glycols - chemistry Propylamines - chemistry Rats Rats, Sprague-Dawley Research Paper Ribonucleic acid RNA RNA Interference RNA, Messenger - genetics RNA, Small Interfering - administration & dosage RNA, Small Interfering - genetics RNA, Small Interfering - pharmacokinetics |
| title | Effect of PEGylation on Biodistribution and Gene Silencing of siRNA/Lipid Nanoparticle Complexes |
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