Regulation of cardiac melusin gene expression by hypertrophic stimuli in the rat

Aim Melusin is an integrin β1‐interacting protein proposed to act as a biomechanical sensor in the heart. We characterized mechanisms and signalling pathways regulating cardiac melusin expression. Methods Infusion of arginine8‐vasopressin (AVP) in Sprague‐Dawley (SD) rats, spontaneously hypertensive...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Acta Physiologica 2013-03, Vol.207 (3), p.470-484
Hauptverfasser:	Aro, J., Tokola, H., Ronkainen, V-P., Koivisto, E., Tenhunen, O., Ilves, M., Szokodi, I., Ruskoaho, H., Rysä, J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Angiotensinogen - genetics Angiotensinogen - metabolism Animals Animals, Newborn Arginine Vasopressin atrial remodelling Cell Line Cytoskeletal Proteins - genetics Cytoskeletal Proteins - metabolism Disease Models, Animal extracellular matrix Gene Expression Regulation Heart Atria - metabolism Heart Ventricles - metabolism Humans Hypertension - chemically induced Hypertension - complications Hypertension - genetics hypertrophy Hypertrophy, Left Ventricular - etiology Hypertrophy, Left Ventricular - genetics Hypertrophy, Left Ventricular - metabolism Hypertrophy, Left Ventricular - pathology Male melusin Mice Muscle Proteins - genetics Muscle Proteins - metabolism Myocytes, Cardiac - metabolism Myocytes, Cardiac - pathology Rats Rats, Inbred SHR Rats, Inbred WKY Rats, Sprague-Dawley Rats, Transgenic Renin - genetics RNA, Messenger - metabolism Signal Transduction Time Factors
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Aim Melusin is an integrin β1‐interacting protein proposed to act as a biomechanical sensor in the heart. We characterized mechanisms and signalling pathways regulating cardiac melusin expression. Methods Infusion of arginine8‐vasopressin (AVP) in Sprague‐Dawley (SD) rats, spontaneously hypertensive rats (SHR) and double transgenic rats (dTGR) harbouring both human angiotensinogen and renin genes as well as infusion of angiotensin II (Ang II) in SD rats were used. The effect of direct left ventricular (LV) wall stretch was analysed by using isolated perfused rat heart preparation. For the cell culture studies, mouse atrial HL‐1 cell line and neonatal rat ventricular myocytes (NRVMs) were used. Results Left atrial melusin mRNA levels increased already after 30 min of AVP infusion. Ang II caused significant upregulation of left atrial melusin mRNA (2.1‐fold at 6 h, P
ISSN:	1748-1708 1748-1716
DOI:	10.1111/apha.12044