Dynamic Anchoring of the 3′-End of the Guide Strand Controls the Target Dissociation of Argonaute–Guide Complex

Argonaute (Ago) is the catalytic core of small RNA-based gene regulation. Despite plenty of mechanistic studies on Ago, the dynamical aspects and the mechanistic determinants of target mRNA binding and dissociation of Ago–guide strand remain unclear. Here, by using single-molecule fluorescence reson...

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Veröffentlicht in:	Journal of the American Chemical Society 2013-11, Vol.135 (45), p.16865-16871
Hauptverfasser:	Jung, Seung-Ryoung, Kim, Eunji, Hwang, Wonseok, Shin, Soochul, Song, Ji-Joon, Hohng, Sungchul
Format:	Artikel
Sprache:	eng
Schlagworte:	Argonaute Proteins - chemistry Argonaute Proteins - metabolism Base Sequence Fluorescence Resonance Energy Transfer Protein Binding Protein Structure, Tertiary RNA, Messenger - chemistry RNA, Messenger - metabolism Thermus thermophilus - chemistry Thermus thermophilus - enzymology Thermus thermophilus - metabolism
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