In-situ seed production after pollination with in-vitro-matured, isolated pollen [Nicotiana tabacum]
Immature tobacco (Nicotiana tabacum L.) pollen has been isolated from anthers in three distinct stages of development, including the microspore stage. In in-vitro cultures, fully functional, mature pollen was obtained. In a germination medium, this pollen produced pollen tubes. After application to...
Gespeichert in:
| Veröffentlicht in: | Planta 1988-11, Vol.176 (2), p.145-148 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 148 |
|---|---|
| container_issue | 2 |
| container_start_page | 145 |
| container_title | Planta |
| container_volume | 176 |
| creator | Moreno, R.M.B. (Vienna Univ. (Austria). Inst. of Microbiology and Genetics) Macke, F Alwen, A Heberle-Bors, E |
| description | Immature tobacco (Nicotiana tabacum L.) pollen has been isolated from anthers in three distinct stages of development, including the microspore stage. In in-vitro cultures, fully functional, mature pollen was obtained. In a germination medium, this pollen produced pollen tubes. After application to stigmas in situ, the in-vitro-matured pollen fertilized ovules, and seeds were produced. Genetic tests with seedlings obtained from pollinations with in-vitro-matured pollen from a transgenic plant revealed normal Mendelian segregation of two marker genes, the neomycin-phosphotransferase II gene and the nopaline-synthase gene. These results are of interest with respect to the control of self-incompatibility, cytoplasmic male sterility and pollen-allergen formation, and it offers an alternative route for gene transfer in those plants which cannot be regenerated in vitro. |
| doi_str_mv | 10.1007/BF00392439 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1458503131</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>23379344</jstor_id><sourcerecordid>23379344</sourcerecordid><originalsourceid>FETCH-LOGICAL-c270t-77ca03ced2c8b87748d4db9004e99c54be81e50e21432a5cbb37dd2e002879e83</originalsourceid><addsrcrecordid>eNpFkM1v1DAQxS0EosvChSMSKIceECIwsZ21faSlX1IFFzghFE3sCbhK4sV2ivjv8bJLqzmMNO83T3qPsecNvGsA1PuTcwBhuBTmAVs1UvCag9QP2aqceQ1GtEfsSUo3AEVU6jE74pJzUBu1Yu5qrpPPS5WIXLWNwS02-zBXOGSK1TaMo5_x3-W3zz8rP9e3PsdQT5iXSO5t5VMYMe-eC0tz9e2TtyF7nLHK2KNdpu9P2aMBx0TPDnvNvp6ffTm9rK8_X1ydfriuLVeQa6UsgrDkuNW9VkpqJ11vACQZY1vZk26oBeK7jNjavhfKOU4AXCtDWqzZ671vyfFroZS7ySdL44gzhSV1jWx1C6Ips2Zv9qiNIaVIQ7eNfsL4p2ug27Xa3bda4FcH36WfyN2h_2sswPEBwGRxHCLO1qc7TglpNmpTsJd77CblEO9thFBGSFn0F3t9wNDhj1gsPp5pfdFwzsVfcfKQlA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1458503131</pqid></control><display><type>article</type><title>In-situ seed production after pollination with in-vitro-matured, isolated pollen [Nicotiana tabacum]</title><source>SpringerNature Journals</source><source>JSTOR</source><creator>Moreno, R.M.B. (Vienna Univ. (Austria). Inst. of Microbiology and Genetics) ; Macke, F ; Alwen, A ; Heberle-Bors, E</creator><creatorcontrib>Moreno, R.M.B. (Vienna Univ. (Austria). Inst. of Microbiology and Genetics) ; Macke, F ; Alwen, A ; Heberle-Bors, E</creatorcontrib><description>Immature tobacco (Nicotiana tabacum L.) pollen has been isolated from anthers in three distinct stages of development, including the microspore stage. In in-vitro cultures, fully functional, mature pollen was obtained. In a germination medium, this pollen produced pollen tubes. After application to stigmas in situ, the in-vitro-matured pollen fertilized ovules, and seeds were produced. Genetic tests with seedlings obtained from pollinations with in-vitro-matured pollen from a transgenic plant revealed normal Mendelian segregation of two marker genes, the neomycin-phosphotransferase II gene and the nopaline-synthase gene. These results are of interest with respect to the control of self-incompatibility, cytoplasmic male sterility and pollen-allergen formation, and it offers an alternative route for gene transfer in those plants which cannot be regenerated in vitro.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/BF00392439</identifier><identifier>PMID: 24220767</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin: Springer-Verlag</publisher><subject>Agronomy. Soil science and plant productions ; Anthers ; Bestaeubung ; Biological and medical sciences ; Biotechnology ; Cultured cells ; Developmental biology ; Economic plant physiology ; Eukaryotic cell cultures ; Flowering, floral biology, reproduction patterns ; Fundamental and applied biological sciences. Psychology ; Gametophytes ; Genetics and breeding of economic plants ; Growth and development ; Haploidy, in vitro culture applications, somatic hybrids ; Immatures ; in vitro ; Methods. Procedures. Technologies ; Microspores ; Miscellaneous ; Nicotiana ; NICOTIANA TABACUM ; Plant breeding: fundamental aspects and methodology ; Plant cells and fungal cells ; Plants ; POLEN ; POLINIZACION ; POLLEN ; Pollenentwicklung ; Pollenreife ; POLLINATION ; POLLINISATION ; PRODUCCION DE SEMILLAS ; PRODUCTION DE SEMENCES ; Samenproduktion ; SEED PRODUCTION ; Starches</subject><ispartof>Planta, 1988-11, Vol.176 (2), p.145-148</ispartof><rights>Springer-Verlag Berlin Heidelberg 1988</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c270t-77ca03ced2c8b87748d4db9004e99c54be81e50e21432a5cbb37dd2e002879e83</citedby><cites>FETCH-LOGICAL-c270t-77ca03ced2c8b87748d4db9004e99c54be81e50e21432a5cbb37dd2e002879e83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23379344$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23379344$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>315,781,785,804,27928,27929,58021,58254</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7349676$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24220767$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moreno, R.M.B. (Vienna Univ. (Austria). Inst. of Microbiology and Genetics)</creatorcontrib><creatorcontrib>Macke, F</creatorcontrib><creatorcontrib>Alwen, A</creatorcontrib><creatorcontrib>Heberle-Bors, E</creatorcontrib><title>In-situ seed production after pollination with in-vitro-matured, isolated pollen [Nicotiana tabacum]</title><title>Planta</title><addtitle>Planta</addtitle><description>Immature tobacco (Nicotiana tabacum L.) pollen has been isolated from anthers in three distinct stages of development, including the microspore stage. In in-vitro cultures, fully functional, mature pollen was obtained. In a germination medium, this pollen produced pollen tubes. After application to stigmas in situ, the in-vitro-matured pollen fertilized ovules, and seeds were produced. Genetic tests with seedlings obtained from pollinations with in-vitro-matured pollen from a transgenic plant revealed normal Mendelian segregation of two marker genes, the neomycin-phosphotransferase II gene and the nopaline-synthase gene. These results are of interest with respect to the control of self-incompatibility, cytoplasmic male sterility and pollen-allergen formation, and it offers an alternative route for gene transfer in those plants which cannot be regenerated in vitro.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Anthers</subject><subject>Bestaeubung</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cultured cells</subject><subject>Developmental biology</subject><subject>Economic plant physiology</subject><subject>Eukaryotic cell cultures</subject><subject>Flowering, floral biology, reproduction patterns</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gametophytes</subject><subject>Genetics and breeding of economic plants</subject><subject>Growth and development</subject><subject>Haploidy, in vitro culture applications, somatic hybrids</subject><subject>Immatures</subject><subject>in vitro</subject><subject>Methods. Procedures. Technologies</subject><subject>Microspores</subject><subject>Miscellaneous</subject><subject>Nicotiana</subject><subject>NICOTIANA TABACUM</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>Plant cells and fungal cells</subject><subject>Plants</subject><subject>POLEN</subject><subject>POLINIZACION</subject><subject>POLLEN</subject><subject>Pollenentwicklung</subject><subject>Pollenreife</subject><subject>POLLINATION</subject><subject>POLLINISATION</subject><subject>PRODUCCION DE SEMILLAS</subject><subject>PRODUCTION DE SEMENCES</subject><subject>Samenproduktion</subject><subject>SEED PRODUCTION</subject><subject>Starches</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><recordid>eNpFkM1v1DAQxS0EosvChSMSKIceECIwsZ21faSlX1IFFzghFE3sCbhK4sV2ivjv8bJLqzmMNO83T3qPsecNvGsA1PuTcwBhuBTmAVs1UvCag9QP2aqceQ1GtEfsSUo3AEVU6jE74pJzUBu1Yu5qrpPPS5WIXLWNwS02-zBXOGSK1TaMo5_x3-W3zz8rP9e3PsdQT5iXSO5t5VMYMe-eC0tz9e2TtyF7nLHK2KNdpu9P2aMBx0TPDnvNvp6ffTm9rK8_X1ydfriuLVeQa6UsgrDkuNW9VkpqJ11vACQZY1vZk26oBeK7jNjavhfKOU4AXCtDWqzZ671vyfFroZS7ySdL44gzhSV1jWx1C6Ips2Zv9qiNIaVIQ7eNfsL4p2ug27Xa3bda4FcH36WfyN2h_2sswPEBwGRxHCLO1qc7TglpNmpTsJd77CblEO9thFBGSFn0F3t9wNDhj1gsPp5pfdFwzsVfcfKQlA</recordid><startdate>198811</startdate><enddate>198811</enddate><creator>Moreno, R.M.B. (Vienna Univ. (Austria). Inst. of Microbiology and Genetics)</creator><creator>Macke, F</creator><creator>Alwen, A</creator><creator>Heberle-Bors, E</creator><general>Springer-Verlag</general><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198811</creationdate><title>In-situ seed production after pollination with in-vitro-matured, isolated pollen [Nicotiana tabacum]</title><author>Moreno, R.M.B. (Vienna Univ. (Austria). Inst. of Microbiology and Genetics) ; Macke, F ; Alwen, A ; Heberle-Bors, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c270t-77ca03ced2c8b87748d4db9004e99c54be81e50e21432a5cbb37dd2e002879e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Anthers</topic><topic>Bestaeubung</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cultured cells</topic><topic>Developmental biology</topic><topic>Economic plant physiology</topic><topic>Eukaryotic cell cultures</topic><topic>Flowering, floral biology, reproduction patterns</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gametophytes</topic><topic>Genetics and breeding of economic plants</topic><topic>Growth and development</topic><topic>Haploidy, in vitro culture applications, somatic hybrids</topic><topic>Immatures</topic><topic>in vitro</topic><topic>Methods. Procedures. Technologies</topic><topic>Microspores</topic><topic>Miscellaneous</topic><topic>Nicotiana</topic><topic>NICOTIANA TABACUM</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>Plant cells and fungal cells</topic><topic>Plants</topic><topic>POLEN</topic><topic>POLINIZACION</topic><topic>POLLEN</topic><topic>Pollenentwicklung</topic><topic>Pollenreife</topic><topic>POLLINATION</topic><topic>POLLINISATION</topic><topic>PRODUCCION DE SEMILLAS</topic><topic>PRODUCTION DE SEMENCES</topic><topic>Samenproduktion</topic><topic>SEED PRODUCTION</topic><topic>Starches</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moreno, R.M.B. (Vienna Univ. (Austria). Inst. of Microbiology and Genetics)</creatorcontrib><creatorcontrib>Macke, F</creatorcontrib><creatorcontrib>Alwen, A</creatorcontrib><creatorcontrib>Heberle-Bors, E</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moreno, R.M.B. (Vienna Univ. (Austria). Inst. of Microbiology and Genetics)</au><au>Macke, F</au><au>Alwen, A</au><au>Heberle-Bors, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In-situ seed production after pollination with in-vitro-matured, isolated pollen [Nicotiana tabacum]</atitle><jtitle>Planta</jtitle><addtitle>Planta</addtitle><date>1988-11</date><risdate>1988</risdate><volume>176</volume><issue>2</issue><spage>145</spage><epage>148</epage><pages>145-148</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>Immature tobacco (Nicotiana tabacum L.) pollen has been isolated from anthers in three distinct stages of development, including the microspore stage. In in-vitro cultures, fully functional, mature pollen was obtained. In a germination medium, this pollen produced pollen tubes. After application to stigmas in situ, the in-vitro-matured pollen fertilized ovules, and seeds were produced. Genetic tests with seedlings obtained from pollinations with in-vitro-matured pollen from a transgenic plant revealed normal Mendelian segregation of two marker genes, the neomycin-phosphotransferase II gene and the nopaline-synthase gene. These results are of interest with respect to the control of self-incompatibility, cytoplasmic male sterility and pollen-allergen formation, and it offers an alternative route for gene transfer in those plants which cannot be regenerated in vitro.</abstract><cop>Berlin</cop><pub>Springer-Verlag</pub><pmid>24220767</pmid><doi>10.1007/BF00392439</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0935 |
| ispartof | Planta, 1988-11, Vol.176 (2), p.145-148 |
| issn | 0032-0935 1432-2048 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1458503131 |
| source | SpringerNature Journals; JSTOR |
| subjects | Agronomy. Soil science and plant productions Anthers Bestaeubung Biological and medical sciences Biotechnology Cultured cells Developmental biology Economic plant physiology Eukaryotic cell cultures Flowering, floral biology, reproduction patterns Fundamental and applied biological sciences. Psychology Gametophytes Genetics and breeding of economic plants Growth and development Haploidy, in vitro culture applications, somatic hybrids Immatures in vitro Methods. Procedures. Technologies Microspores Miscellaneous Nicotiana NICOTIANA TABACUM Plant breeding: fundamental aspects and methodology Plant cells and fungal cells Plants POLEN POLINIZACION POLLEN Pollenentwicklung Pollenreife POLLINATION POLLINISATION PRODUCCION DE SEMILLAS PRODUCTION DE SEMENCES Samenproduktion SEED PRODUCTION Starches |
| title | In-situ seed production after pollination with in-vitro-matured, isolated pollen [Nicotiana tabacum] |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-17T04%3A53%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In-situ%20seed%20production%20after%20pollination%20with%20in-vitro-matured,%20isolated%20pollen%20%5BNicotiana%20tabacum%5D&rft.jtitle=Planta&rft.au=Moreno,%20R.M.B.%20(Vienna%20Univ.%20(Austria).%20Inst.%20of%20Microbiology%20and%20Genetics)&rft.date=1988-11&rft.volume=176&rft.issue=2&rft.spage=145&rft.epage=148&rft.pages=145-148&rft.issn=0032-0935&rft.eissn=1432-2048&rft.coden=PLANAB&rft_id=info:doi/10.1007/BF00392439&rft_dat=%3Cjstor_proqu%3E23379344%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1458503131&rft_id=info:pmid/24220767&rft_jstor_id=23379344&rfr_iscdi=true |