pEmu: an improved promoter for gene expression in cereal cells
A recombinant promoter, pEmu, has been constructed to give a high level of gene expression in monocots. It is based on a truncated maize Adh1 promoter, with multiple copies of the Anaerobic Responsive Element from the maize Adh1 gene and ocs-elements from the octopine synthase gene of Agrobacterium...
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| Veröffentlicht in: | Theoretical and applied genetics 1991-05, Vol.81 (5), p.581-588 |
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| container_title | Theoretical and applied genetics |
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| creator | Last, D.I Brettell, R.I.S Chamberlain, D.A Chaudhury, A.M Larkin, P.J Marsh, E.L Peacock, W.J Dennis, E.S |
| description | A recombinant promoter, pEmu, has been constructed to give a high level of gene expression in monocots. It is based on a truncated maize Adh1 promoter, with multiple copies of the Anaerobic Responsive Element from the maize Adh1 gene and ocs-elements from the octopine synthase gene of Agrobacterium tumefaciens. The pEmu promoter was one of 12 different promoter constructs that were linked to the beta-glucuronidase (GUS) marker gene. Promoter activity was measured 48 h after introduction of the constructs into protoplasts of five different monocot species [wheat, maize, rice, einkorn (Triticum monococcum), and Lolium multiflorum] and one dicot (Nicotiana plumbaginifolia). In suspension cell protoplasts, the most highly expressing construct (pEmuGN) gave 10- to 50-fold higher expression than the CaMV 35S promoter in all the monocot species. The pEmu promoter should be valuable where a high level of gene expression is required in monocots. The pEmu promoter showed instability in several widely used Escherichia coli strains but was stable in a recA, recD strain AC001, which is described. Another construct, P4OCS delta 35SIGN, gave a tenfold increase in expression over the CaMV 35S promoter in dicot (Nicotiana plumbaginifolia) protoplasts. |
| doi_str_mv | 10.1007/BF00226722 |
| format | Article |
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It is based on a truncated maize Adh1 promoter, with multiple copies of the Anaerobic Responsive Element from the maize Adh1 gene and ocs-elements from the octopine synthase gene of Agrobacterium tumefaciens. The pEmu promoter was one of 12 different promoter constructs that were linked to the beta-glucuronidase (GUS) marker gene. Promoter activity was measured 48 h after introduction of the constructs into protoplasts of five different monocot species [wheat, maize, rice, einkorn (Triticum monococcum), and Lolium multiflorum] and one dicot (Nicotiana plumbaginifolia). In suspension cell protoplasts, the most highly expressing construct (pEmuGN) gave 10- to 50-fold higher expression than the CaMV 35S promoter in all the monocot species. The pEmu promoter should be valuable where a high level of gene expression is required in monocots. The pEmu promoter showed instability in several widely used Escherichia coli strains but was stable in a recA, recD strain AC001, which is described. Another construct, P4OCS delta 35SIGN, gave a tenfold increase in expression over the CaMV 35S promoter in dicot (Nicotiana plumbaginifolia) protoplasts.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/BF00226722</identifier><identifier>PMID: 24221371</identifier><identifier>CODEN: THAGA6</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>adh1 promoter ; beta-glucuronidase ; Biological and medical sciences ; Classical genetics, quantitative genetics, hybrids ; direct DNA uptake ; electroporation ; Fundamental and applied biological sciences. Psychology ; gene expression ; genetic transformation ; Genetics of eukaryotes. Biological and molecular evolution ; Lolium multiflorum ; Nicotiana plumbaginifolia ; Oryza sativa ; promoter regions ; protoplasts ; Pteridophyta, spermatophyta ; recombinant DNA ; recombinant promoters ; reporter genes ; Triticum aestivum ; Triticum monococcum ; vectors ; Vegetals ; Zea mays</subject><ispartof>Theoretical and applied genetics, 1991-05, Vol.81 (5), p.581-588</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-941a2ba0c817c2a432350c3a8e2fabb0f2f617dd74d9793eb55972b71dc359053</citedby><cites>FETCH-LOGICAL-c379t-941a2ba0c817c2a432350c3a8e2fabb0f2f617dd74d9793eb55972b71dc359053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4949937$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24221371$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Last, D.I</creatorcontrib><creatorcontrib>Brettell, R.I.S</creatorcontrib><creatorcontrib>Chamberlain, D.A</creatorcontrib><creatorcontrib>Chaudhury, A.M</creatorcontrib><creatorcontrib>Larkin, P.J</creatorcontrib><creatorcontrib>Marsh, E.L</creatorcontrib><creatorcontrib>Peacock, W.J</creatorcontrib><creatorcontrib>Dennis, E.S</creatorcontrib><title>pEmu: an improved promoter for gene expression in cereal cells</title><title>Theoretical and applied genetics</title><addtitle>Theor Appl Genet</addtitle><description>A recombinant promoter, pEmu, has been constructed to give a high level of gene expression in monocots. It is based on a truncated maize Adh1 promoter, with multiple copies of the Anaerobic Responsive Element from the maize Adh1 gene and ocs-elements from the octopine synthase gene of Agrobacterium tumefaciens. The pEmu promoter was one of 12 different promoter constructs that were linked to the beta-glucuronidase (GUS) marker gene. Promoter activity was measured 48 h after introduction of the constructs into protoplasts of five different monocot species [wheat, maize, rice, einkorn (Triticum monococcum), and Lolium multiflorum] and one dicot (Nicotiana plumbaginifolia). In suspension cell protoplasts, the most highly expressing construct (pEmuGN) gave 10- to 50-fold higher expression than the CaMV 35S promoter in all the monocot species. The pEmu promoter should be valuable where a high level of gene expression is required in monocots. The pEmu promoter showed instability in several widely used Escherichia coli strains but was stable in a recA, recD strain AC001, which is described. Another construct, P4OCS delta 35SIGN, gave a tenfold increase in expression over the CaMV 35S promoter in dicot (Nicotiana plumbaginifolia) protoplasts.</description><subject>adh1 promoter</subject><subject>beta-glucuronidase</subject><subject>Biological and medical sciences</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>direct DNA uptake</subject><subject>electroporation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>genetic transformation</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Lolium multiflorum</subject><subject>Nicotiana plumbaginifolia</subject><subject>Oryza sativa</subject><subject>promoter regions</subject><subject>protoplasts</subject><subject>Pteridophyta, spermatophyta</subject><subject>recombinant DNA</subject><subject>recombinant promoters</subject><subject>reporter genes</subject><subject>Triticum aestivum</subject><subject>Triticum monococcum</subject><subject>vectors</subject><subject>Vegetals</subject><subject>Zea mays</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNpF0M1KAzEUBeAgiq3VjQ-gs3AhwujNTdJMXAharAoFF9r1kMkkZWR-atIRfXtTWuvqLO7H5XAIOaVwTQHkzcMUAHEsEffIkHKGKSLHfTIE4JAKKXBAjkL4gMgEsEMyiGekTNIhuVs-Nv1totukapa--7JlEqPpVtYnrvPJwrY2sd9Lb0OouqjaxFhvdR2jrsMxOXC6DvZkmyMynz6-T57T2evTy-R-lhom1SpVnGosNJiMSoM6VmQCDNOZRaeLAhy6MZVlKXmppGK2EEJJLCQtDRMKBBuRy83fWO6zt2GVN1VYN9Ct7fqQUy4yAZjJNb3aUOO7ELx1-dJXjfY_OYV8vVf-v1fEZ9u_fdHYckf_BorgYgt0MLp2XremCjvHFVeKycjON8zpLtcLH8n8DYEyoJKxjAL7BWuieOc</recordid><startdate>199105</startdate><enddate>199105</enddate><creator>Last, D.I</creator><creator>Brettell, R.I.S</creator><creator>Chamberlain, D.A</creator><creator>Chaudhury, A.M</creator><creator>Larkin, P.J</creator><creator>Marsh, E.L</creator><creator>Peacock, W.J</creator><creator>Dennis, E.S</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199105</creationdate><title>pEmu: an improved promoter for gene expression in cereal cells</title><author>Last, D.I ; Brettell, R.I.S ; Chamberlain, D.A ; Chaudhury, A.M ; Larkin, P.J ; Marsh, E.L ; Peacock, W.J ; Dennis, E.S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-941a2ba0c817c2a432350c3a8e2fabb0f2f617dd74d9793eb55972b71dc359053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>adh1 promoter</topic><topic>beta-glucuronidase</topic><topic>Biological and medical sciences</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>direct DNA uptake</topic><topic>electroporation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>genetic transformation</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Lolium multiflorum</topic><topic>Nicotiana plumbaginifolia</topic><topic>Oryza sativa</topic><topic>promoter regions</topic><topic>protoplasts</topic><topic>Pteridophyta, spermatophyta</topic><topic>recombinant DNA</topic><topic>recombinant promoters</topic><topic>reporter genes</topic><topic>Triticum aestivum</topic><topic>Triticum monococcum</topic><topic>vectors</topic><topic>Vegetals</topic><topic>Zea mays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Last, D.I</creatorcontrib><creatorcontrib>Brettell, R.I.S</creatorcontrib><creatorcontrib>Chamberlain, D.A</creatorcontrib><creatorcontrib>Chaudhury, A.M</creatorcontrib><creatorcontrib>Larkin, P.J</creatorcontrib><creatorcontrib>Marsh, E.L</creatorcontrib><creatorcontrib>Peacock, W.J</creatorcontrib><creatorcontrib>Dennis, E.S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theoretical and applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Last, D.I</au><au>Brettell, R.I.S</au><au>Chamberlain, D.A</au><au>Chaudhury, A.M</au><au>Larkin, P.J</au><au>Marsh, E.L</au><au>Peacock, W.J</au><au>Dennis, E.S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>pEmu: an improved promoter for gene expression in cereal cells</atitle><jtitle>Theoretical and applied genetics</jtitle><addtitle>Theor Appl Genet</addtitle><date>1991-05</date><risdate>1991</risdate><volume>81</volume><issue>5</issue><spage>581</spage><epage>588</epage><pages>581-588</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><coden>THAGA6</coden><abstract>A recombinant promoter, pEmu, has been constructed to give a high level of gene expression in monocots. It is based on a truncated maize Adh1 promoter, with multiple copies of the Anaerobic Responsive Element from the maize Adh1 gene and ocs-elements from the octopine synthase gene of Agrobacterium tumefaciens. The pEmu promoter was one of 12 different promoter constructs that were linked to the beta-glucuronidase (GUS) marker gene. Promoter activity was measured 48 h after introduction of the constructs into protoplasts of five different monocot species [wheat, maize, rice, einkorn (Triticum monococcum), and Lolium multiflorum] and one dicot (Nicotiana plumbaginifolia). In suspension cell protoplasts, the most highly expressing construct (pEmuGN) gave 10- to 50-fold higher expression than the CaMV 35S promoter in all the monocot species. The pEmu promoter should be valuable where a high level of gene expression is required in monocots. The pEmu promoter showed instability in several widely used Escherichia coli strains but was stable in a recA, recD strain AC001, which is described. Another construct, P4OCS delta 35SIGN, gave a tenfold increase in expression over the CaMV 35S promoter in dicot (Nicotiana plumbaginifolia) protoplasts.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>24221371</pmid><doi>10.1007/BF00226722</doi><tpages>8</tpages></addata></record> |
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| subjects | adh1 promoter beta-glucuronidase Biological and medical sciences Classical genetics, quantitative genetics, hybrids direct DNA uptake electroporation Fundamental and applied biological sciences. Psychology gene expression genetic transformation Genetics of eukaryotes. Biological and molecular evolution Lolium multiflorum Nicotiana plumbaginifolia Oryza sativa promoter regions protoplasts Pteridophyta, spermatophyta recombinant DNA recombinant promoters reporter genes Triticum aestivum Triticum monococcum vectors Vegetals Zea mays |
| title | pEmu: an improved promoter for gene expression in cereal cells |
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