Genetic transformation of chrysanthemum using wild type Agrobacterium strains; strain and cultivar specificity

To develop an Agrobacterium mediated transformation protocol for chrysanthemum we studied the transformation efficiency of commonly used A.tumefaciens strains on 14 genotypes by comparing tumour size and frequency. One genotype was analyzed in detail using 14 strains of both A.tumefaciens and A.rhiz...

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Veröffentlicht in:	Plant cell reports 1991, Vol.9 (9), p.505-508
Hauptverfasser:	VAN WORDRAGEN, M. F, JAN DE JONG, HUITEMA, H. B. M, DONS, H. J. M
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Methods. Procedures. Technologies Transgenic animals and transgenic plants Transgenic plants
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container_end_page	508
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container_title	Plant cell reports
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creator	VAN WORDRAGEN, M. F JAN DE JONG HUITEMA, H. B. M DONS, H. J. M
description	To develop an Agrobacterium mediated transformation protocol for chrysanthemum we studied the transformation efficiency of commonly used A.tumefaciens strains on 14 genotypes by comparing tumour size and frequency. One genotype was analyzed in detail using 14 strains of both A.tumefaciens and A.rhizogenes. Only a few genotype/strain combinations resulted in significant tumour formation. Especially 0-type strains were highly efficient. An 0-type strain was used to transfer genes for neomycine phosphotransferase (NPT II) and ß-glucuronidase (GUS) to a susceptible cultivar. Transfer of the GUS gene was confirmed by using the Polymerase Chain Reaction (PCR).
doi_str_mv	10.1007/bf00232106
format	Article
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subjects	Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Methods. Procedures. Technologies Transgenic animals and transgenic plants Transgenic plants
title	Genetic transformation of chrysanthemum using wild type Agrobacterium strains; strain and cultivar specificity
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