High-resolution microscopy for biological specimens via cathodoluminescence of Eu- and Zn-doped Y2O3 nanophosphors
High-resolution microscopy for biological specimens was performed using cathodoluminescence (CL) of Y(2)O(3):Eu, Zn nanophosphors, which have high CL intensity due to the incorporation of Zn. The intensity of Y(2)O(3):Eu nanophosphors at low acceleration voltage (3 kV) was increased by adding Zn. Th...
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| Veröffentlicht in: | Optics express 2013-11, Vol.21 (22), p.25655-25663 |
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| container_title | Optics express |
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| creator | Furukawa, Taichi Niioka, Hirohiko Ichimiya, Masayoshi Nagata, Tomohiro Ashida, Masaaki Araki, Tsutomu Hashimoto, Mamoru |
| description | High-resolution microscopy for biological specimens was performed using cathodoluminescence (CL) of Y(2)O(3):Eu, Zn nanophosphors, which have high CL intensity due to the incorporation of Zn. The intensity of Y(2)O(3):Eu nanophosphors at low acceleration voltage (3 kV) was increased by adding Zn. The CL intensity was high enough for imaging even with a phosphor size as small as about 30 nm. The results show the possibility of using CL microscopy for biological specimens at single-protein-scale resolution. CL imaging of HeLa cells containing laser-ablated Y(2)O(3):Eu, Zn nanophosphors achieved a spatial resolution of a few tens of nanometers. Y(2)O(3):Eu, Zn nanophosphors in HeLa cells were also imaged with 254 nm ultraviolet light excitation. The results suggest that correlative microscopy using CL, secondary electrons and fluorescence imaging could enable multi-scale investigation of molecular localization from the nanoscale to the microscale. |
| doi_str_mv | 10.1364/OE.21.025655 |
| format | Article |
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The intensity of Y(2)O(3):Eu nanophosphors at low acceleration voltage (3 kV) was increased by adding Zn. The CL intensity was high enough for imaging even with a phosphor size as small as about 30 nm. The results show the possibility of using CL microscopy for biological specimens at single-protein-scale resolution. CL imaging of HeLa cells containing laser-ablated Y(2)O(3):Eu, Zn nanophosphors achieved a spatial resolution of a few tens of nanometers. Y(2)O(3):Eu, Zn nanophosphors in HeLa cells were also imaged with 254 nm ultraviolet light excitation. 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The intensity of Y(2)O(3):Eu nanophosphors at low acceleration voltage (3 kV) was increased by adding Zn. The CL intensity was high enough for imaging even with a phosphor size as small as about 30 nm. The results show the possibility of using CL microscopy for biological specimens at single-protein-scale resolution. CL imaging of HeLa cells containing laser-ablated Y(2)O(3):Eu, Zn nanophosphors achieved a spatial resolution of a few tens of nanometers. Y(2)O(3):Eu, Zn nanophosphors in HeLa cells were also imaged with 254 nm ultraviolet light excitation. The results suggest that correlative microscopy using CL, secondary electrons and fluorescence imaging could enable multi-scale investigation of molecular localization from the nanoscale to the microscale.</description><subject>Contrast Media</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Image Enhancement - methods</subject><subject>Lighting - methods</subject><subject>Luminescent Measurements - methods</subject><subject>Metal Nanoparticles</subject><subject>Microscopy, Fluorescence - methods</subject><subject>Molecular Imaging - methods</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Neoplasm Proteins - ultrastructure</subject><issn>1094-4087</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1UD1PwzAUtJAQLYWNGXlkSfHzR-KMqCoUqVIWGGCJHNtpjRI72AlS_z2RKMPdG-5DT4fQHZA1sJw_Vts1hTWhIhfiAi2BlDzjRBYLdJ3SFyHAi7K4QgvKKeRFSZYo7tzhmEWbQjeNLnjcOx1D0mE44TZE3LjQhYPTqsNpsNr11if84xTWajwGM6d6523S1muLQ4u3U4aVN_jTZyYM1uAPWjHslQ_DMaQZMd2gy1Z1yd6e7wq9P2_fNrtsX728bp722UABxkxwwnJKm5kMaDBSAoASzJoCmoILoYiVKhdWEdbKFtpZkSIvGpIz3UjOVujhr3eI4Xuyaax7Nz_adcrbMKUauJAgOZflbL0_W6emt6YeoutVPNX_O7FftW1oPg</recordid><startdate>20131104</startdate><enddate>20131104</enddate><creator>Furukawa, Taichi</creator><creator>Niioka, Hirohiko</creator><creator>Ichimiya, Masayoshi</creator><creator>Nagata, Tomohiro</creator><creator>Ashida, Masaaki</creator><creator>Araki, Tsutomu</creator><creator>Hashimoto, Mamoru</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20131104</creationdate><title>High-resolution microscopy for biological specimens via cathodoluminescence of Eu- and Zn-doped Y2O3 nanophosphors</title><author>Furukawa, Taichi ; Niioka, Hirohiko ; Ichimiya, Masayoshi ; Nagata, Tomohiro ; Ashida, Masaaki ; Araki, Tsutomu ; Hashimoto, Mamoru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p211t-5403622b362d1c1d88111a53ed71b7455a0e8a65ea03f8f1f3ed8567b063cb843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Contrast Media</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Image Enhancement - methods</topic><topic>Lighting - methods</topic><topic>Luminescent Measurements - methods</topic><topic>Metal Nanoparticles</topic><topic>Microscopy, Fluorescence - methods</topic><topic>Molecular Imaging - methods</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Neoplasm Proteins - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Furukawa, Taichi</creatorcontrib><creatorcontrib>Niioka, Hirohiko</creatorcontrib><creatorcontrib>Ichimiya, Masayoshi</creatorcontrib><creatorcontrib>Nagata, Tomohiro</creatorcontrib><creatorcontrib>Ashida, Masaaki</creatorcontrib><creatorcontrib>Araki, Tsutomu</creatorcontrib><creatorcontrib>Hashimoto, Mamoru</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Optics express</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Furukawa, Taichi</au><au>Niioka, Hirohiko</au><au>Ichimiya, Masayoshi</au><au>Nagata, Tomohiro</au><au>Ashida, Masaaki</au><au>Araki, Tsutomu</au><au>Hashimoto, Mamoru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-resolution microscopy for biological specimens via cathodoluminescence of Eu- and Zn-doped Y2O3 nanophosphors</atitle><jtitle>Optics express</jtitle><addtitle>Opt Express</addtitle><date>2013-11-04</date><risdate>2013</risdate><volume>21</volume><issue>22</issue><spage>25655</spage><epage>25663</epage><pages>25655-25663</pages><eissn>1094-4087</eissn><abstract>High-resolution microscopy for biological specimens was performed using cathodoluminescence (CL) of Y(2)O(3):Eu, Zn nanophosphors, which have high CL intensity due to the incorporation of Zn. The intensity of Y(2)O(3):Eu nanophosphors at low acceleration voltage (3 kV) was increased by adding Zn. The CL intensity was high enough for imaging even with a phosphor size as small as about 30 nm. The results show the possibility of using CL microscopy for biological specimens at single-protein-scale resolution. CL imaging of HeLa cells containing laser-ablated Y(2)O(3):Eu, Zn nanophosphors achieved a spatial resolution of a few tens of nanometers. Y(2)O(3):Eu, Zn nanophosphors in HeLa cells were also imaged with 254 nm ultraviolet light excitation. The results suggest that correlative microscopy using CL, secondary electrons and fluorescence imaging could enable multi-scale investigation of molecular localization from the nanoscale to the microscale.</abstract><cop>United States</cop><pmid>24216790</pmid><doi>10.1364/OE.21.025655</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Contrast Media HeLa Cells Humans Image Enhancement - methods Lighting - methods Luminescent Measurements - methods Metal Nanoparticles Microscopy, Fluorescence - methods Molecular Imaging - methods Neoplasm Proteins - metabolism Neoplasm Proteins - ultrastructure |
| title | High-resolution microscopy for biological specimens via cathodoluminescence of Eu- and Zn-doped Y2O3 nanophosphors |
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