Improvement for identification of heterophile antibody interference and AFP hook effect in immunoassays with multiplex suspension bead array system
A variety of immunoassays including multiplex suspension bead array have been developed for tumor marker detections; however, these assays could be compromised in their sensitivity and specificity by well-known heterophile antibody interference and hook effect. Using Luminex® multiplex suspension be...
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| Veröffentlicht in: | Clinica chimica acta 2013-11, Vol.426, p.68-74 |
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| creator | Wang, Yajie Yu, Jinsheng Ren, Yuan Liu, Li Li, Haowen Guo, Anchen Shi, Congning Fang, Fang Juehne, Twyla Yao, Jianer Yang, Enhuan Zhou, Xuelei Kang, Xixiong |
| description | A variety of immunoassays including multiplex suspension bead array have been developed for tumor marker detections; however, these assays could be compromised in their sensitivity and specificity by well-known heterophile antibody interference and hook effect.
Using Luminex® multiplex suspension bead arrays, we modified protocols with two newly-developed solutions that can identify heterophile antibody interference and AFP hook effect. Effectiveness of the two solutions was assessed in serum samples from patients.
Concentrations of 9 tumor markers in heterophile antibody positive samples assayed with Solution A, containing murine monoclonal antibodies and mouse serum, were significantly reduced when compared with those false high signals assayed without Solution A (all p97% high specific reactivity.•A unique model for the identification of AFP hook effect was defined successfully.•Hook effect of AFP was effectively identified using AFP antigen beads.•Blocking Solution A was effective in eliminating heterophile interference. |
| doi_str_mv | 10.1016/j.cca.2013.09.005 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1458183468</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0009898113003434</els_id><sourcerecordid>1458183468</sourcerecordid><originalsourceid>FETCH-LOGICAL-c353t-6298fab88cf2e0e09027712a0b466871939c6ef5943e998d0c9944830e5818a93</originalsourceid><addsrcrecordid>eNp9kcFu1DAQhi0EokvhAbggH7kkjGNv1hanqqJQqRIc4Gw5zljrJY6DnZTmOXhhHG3LkZPHM9_8lv-fkLcMagas_XCqrTV1A4zXoGqA_TOyY_LAKy5U85zsAEBVUkl2QV7lfCpXAS17SS4aAYJJxnbkz22YUrzHgONMXUzU96Xyzlsz-zjS6OgRZ0xxOvoBqSmzLvYr9WNpOkw42q3b06ubb_QY40-KzqGdC0B9CMsYTc5mzfS3n480LMPspwEfaF7yhGPenujQ9NSkZFaa1zxjeE1eODNkfPN4XpIfN5--X3-p7r5-vr2-uqss3_O5ahslnemktK5BQFDQHA6sMdCJtpUHpriyLbq9EhyVkj1YpYSQHHAvmTSKX5L3Z93iwK8F86yDzxaHwYwYl6yZ2EAuWllQdkZtijkndHpKPpi0agZ6y0KfdMlCb1loULpkUXbePcovXcD-38aT-QX4eAawfPLeY9LZ-s3Q3qdioe6j_4_8XxcynE8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1458183468</pqid></control><display><type>article</type><title>Improvement for identification of heterophile antibody interference and AFP hook effect in immunoassays with multiplex suspension bead array system</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Wang, Yajie ; Yu, Jinsheng ; Ren, Yuan ; Liu, Li ; Li, Haowen ; Guo, Anchen ; Shi, Congning ; Fang, Fang ; Juehne, Twyla ; Yao, Jianer ; Yang, Enhuan ; Zhou, Xuelei ; Kang, Xixiong</creator><creatorcontrib>Wang, Yajie ; Yu, Jinsheng ; Ren, Yuan ; Liu, Li ; Li, Haowen ; Guo, Anchen ; Shi, Congning ; Fang, Fang ; Juehne, Twyla ; Yao, Jianer ; Yang, Enhuan ; Zhou, Xuelei ; Kang, Xixiong</creatorcontrib><description>A variety of immunoassays including multiplex suspension bead array have been developed for tumor marker detections; however, these assays could be compromised in their sensitivity and specificity by well-known heterophile antibody interference and hook effect.
Using Luminex® multiplex suspension bead arrays, we modified protocols with two newly-developed solutions that can identify heterophile antibody interference and AFP hook effect. Effectiveness of the two solutions was assessed in serum samples from patients.
Concentrations of 9 tumor markers in heterophile antibody positive samples assayed with Solution A, containing murine monoclonal antibodies and mouse serum, were significantly reduced when compared with those false high signals assayed without Solution A (all p<0.01). With incorporation of Solution H (fluorescent beads linked with AFP antigen), a new strategy for identification of AFP hook effect was established, and with this strategy AFP hook effect was identified effectively in serum samples with very high levels of AFP.
Two proprietary solutions improve the identification of heterophile antibody interference and AFP hook effect. With these solutions, multiplex suspension bead arrays provide more reliable testing results in tumor marker detection where complex clinical serum samples are used.
[Display omitted]
•All 8 detection antibodies for tumor markers had >97% high specific reactivity.•A unique model for the identification of AFP hook effect was defined successfully.•Hook effect of AFP was effectively identified using AFP antigen beads.•Blocking Solution A was effective in eliminating heterophile interference.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/j.cca.2013.09.005</identifier><identifier>PMID: 24041811</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>alpha-Fetoproteins - analysis ; Antibodies, Heterophile - blood ; Antibody Specificity ; Biomarkers, Tumor - blood ; Heterophile antibody ; Hook effect ; Humans ; Immunoassay ; Luminescent Measurements ; Multiplex suspension bead array ; Neoplasms - blood ; Neoplasms - diagnosis ; Sensitivity and Specificity ; Tumor marker</subject><ispartof>Clinica chimica acta, 2013-11, Vol.426, p.68-74</ispartof><rights>2013 Elsevier B.V.</rights><rights>2013.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-6298fab88cf2e0e09027712a0b466871939c6ef5943e998d0c9944830e5818a93</citedby><cites>FETCH-LOGICAL-c353t-6298fab88cf2e0e09027712a0b466871939c6ef5943e998d0c9944830e5818a93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cca.2013.09.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24041811$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Yajie</creatorcontrib><creatorcontrib>Yu, Jinsheng</creatorcontrib><creatorcontrib>Ren, Yuan</creatorcontrib><creatorcontrib>Liu, Li</creatorcontrib><creatorcontrib>Li, Haowen</creatorcontrib><creatorcontrib>Guo, Anchen</creatorcontrib><creatorcontrib>Shi, Congning</creatorcontrib><creatorcontrib>Fang, Fang</creatorcontrib><creatorcontrib>Juehne, Twyla</creatorcontrib><creatorcontrib>Yao, Jianer</creatorcontrib><creatorcontrib>Yang, Enhuan</creatorcontrib><creatorcontrib>Zhou, Xuelei</creatorcontrib><creatorcontrib>Kang, Xixiong</creatorcontrib><title>Improvement for identification of heterophile antibody interference and AFP hook effect in immunoassays with multiplex suspension bead array system</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>A variety of immunoassays including multiplex suspension bead array have been developed for tumor marker detections; however, these assays could be compromised in their sensitivity and specificity by well-known heterophile antibody interference and hook effect.
Using Luminex® multiplex suspension bead arrays, we modified protocols with two newly-developed solutions that can identify heterophile antibody interference and AFP hook effect. Effectiveness of the two solutions was assessed in serum samples from patients.
Concentrations of 9 tumor markers in heterophile antibody positive samples assayed with Solution A, containing murine monoclonal antibodies and mouse serum, were significantly reduced when compared with those false high signals assayed without Solution A (all p<0.01). With incorporation of Solution H (fluorescent beads linked with AFP antigen), a new strategy for identification of AFP hook effect was established, and with this strategy AFP hook effect was identified effectively in serum samples with very high levels of AFP.
Two proprietary solutions improve the identification of heterophile antibody interference and AFP hook effect. With these solutions, multiplex suspension bead arrays provide more reliable testing results in tumor marker detection where complex clinical serum samples are used.
[Display omitted]
•All 8 detection antibodies for tumor markers had >97% high specific reactivity.•A unique model for the identification of AFP hook effect was defined successfully.•Hook effect of AFP was effectively identified using AFP antigen beads.•Blocking Solution A was effective in eliminating heterophile interference.</description><subject>alpha-Fetoproteins - analysis</subject><subject>Antibodies, Heterophile - blood</subject><subject>Antibody Specificity</subject><subject>Biomarkers, Tumor - blood</subject><subject>Heterophile antibody</subject><subject>Hook effect</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Luminescent Measurements</subject><subject>Multiplex suspension bead array</subject><subject>Neoplasms - blood</subject><subject>Neoplasms - diagnosis</subject><subject>Sensitivity and Specificity</subject><subject>Tumor marker</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAQhi0EokvhAbggH7kkjGNv1hanqqJQqRIc4Gw5zljrJY6DnZTmOXhhHG3LkZPHM9_8lv-fkLcMagas_XCqrTV1A4zXoGqA_TOyY_LAKy5U85zsAEBVUkl2QV7lfCpXAS17SS4aAYJJxnbkz22YUrzHgONMXUzU96Xyzlsz-zjS6OgRZ0xxOvoBqSmzLvYr9WNpOkw42q3b06ubb_QY40-KzqGdC0B9CMsYTc5mzfS3n480LMPspwEfaF7yhGPenujQ9NSkZFaa1zxjeE1eODNkfPN4XpIfN5--X3-p7r5-vr2-uqss3_O5ahslnemktK5BQFDQHA6sMdCJtpUHpriyLbq9EhyVkj1YpYSQHHAvmTSKX5L3Z93iwK8F86yDzxaHwYwYl6yZ2EAuWllQdkZtijkndHpKPpi0agZ6y0KfdMlCb1loULpkUXbePcovXcD-38aT-QX4eAawfPLeY9LZ-s3Q3qdioe6j_4_8XxcynE8</recordid><startdate>20131115</startdate><enddate>20131115</enddate><creator>Wang, Yajie</creator><creator>Yu, Jinsheng</creator><creator>Ren, Yuan</creator><creator>Liu, Li</creator><creator>Li, Haowen</creator><creator>Guo, Anchen</creator><creator>Shi, Congning</creator><creator>Fang, Fang</creator><creator>Juehne, Twyla</creator><creator>Yao, Jianer</creator><creator>Yang, Enhuan</creator><creator>Zhou, Xuelei</creator><creator>Kang, Xixiong</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20131115</creationdate><title>Improvement for identification of heterophile antibody interference and AFP hook effect in immunoassays with multiplex suspension bead array system</title><author>Wang, Yajie ; Yu, Jinsheng ; Ren, Yuan ; Liu, Li ; Li, Haowen ; Guo, Anchen ; Shi, Congning ; Fang, Fang ; Juehne, Twyla ; Yao, Jianer ; Yang, Enhuan ; Zhou, Xuelei ; Kang, Xixiong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-6298fab88cf2e0e09027712a0b466871939c6ef5943e998d0c9944830e5818a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>alpha-Fetoproteins - analysis</topic><topic>Antibodies, Heterophile - blood</topic><topic>Antibody Specificity</topic><topic>Biomarkers, Tumor - blood</topic><topic>Heterophile antibody</topic><topic>Hook effect</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Luminescent Measurements</topic><topic>Multiplex suspension bead array</topic><topic>Neoplasms - blood</topic><topic>Neoplasms - diagnosis</topic><topic>Sensitivity and Specificity</topic><topic>Tumor marker</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yajie</creatorcontrib><creatorcontrib>Yu, Jinsheng</creatorcontrib><creatorcontrib>Ren, Yuan</creatorcontrib><creatorcontrib>Liu, Li</creatorcontrib><creatorcontrib>Li, Haowen</creatorcontrib><creatorcontrib>Guo, Anchen</creatorcontrib><creatorcontrib>Shi, Congning</creatorcontrib><creatorcontrib>Fang, Fang</creatorcontrib><creatorcontrib>Juehne, Twyla</creatorcontrib><creatorcontrib>Yao, Jianer</creatorcontrib><creatorcontrib>Yang, Enhuan</creatorcontrib><creatorcontrib>Zhou, Xuelei</creatorcontrib><creatorcontrib>Kang, Xixiong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yajie</au><au>Yu, Jinsheng</au><au>Ren, Yuan</au><au>Liu, Li</au><au>Li, Haowen</au><au>Guo, Anchen</au><au>Shi, Congning</au><au>Fang, Fang</au><au>Juehne, Twyla</au><au>Yao, Jianer</au><au>Yang, Enhuan</au><au>Zhou, Xuelei</au><au>Kang, Xixiong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improvement for identification of heterophile antibody interference and AFP hook effect in immunoassays with multiplex suspension bead array system</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>2013-11-15</date><risdate>2013</risdate><volume>426</volume><spage>68</spage><epage>74</epage><pages>68-74</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>A variety of immunoassays including multiplex suspension bead array have been developed for tumor marker detections; however, these assays could be compromised in their sensitivity and specificity by well-known heterophile antibody interference and hook effect.
Using Luminex® multiplex suspension bead arrays, we modified protocols with two newly-developed solutions that can identify heterophile antibody interference and AFP hook effect. Effectiveness of the two solutions was assessed in serum samples from patients.
Concentrations of 9 tumor markers in heterophile antibody positive samples assayed with Solution A, containing murine monoclonal antibodies and mouse serum, were significantly reduced when compared with those false high signals assayed without Solution A (all p<0.01). With incorporation of Solution H (fluorescent beads linked with AFP antigen), a new strategy for identification of AFP hook effect was established, and with this strategy AFP hook effect was identified effectively in serum samples with very high levels of AFP.
Two proprietary solutions improve the identification of heterophile antibody interference and AFP hook effect. With these solutions, multiplex suspension bead arrays provide more reliable testing results in tumor marker detection where complex clinical serum samples are used.
[Display omitted]
•All 8 detection antibodies for tumor markers had >97% high specific reactivity.•A unique model for the identification of AFP hook effect was defined successfully.•Hook effect of AFP was effectively identified using AFP antigen beads.•Blocking Solution A was effective in eliminating heterophile interference.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>24041811</pmid><doi>10.1016/j.cca.2013.09.005</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | alpha-Fetoproteins - analysis Antibodies, Heterophile - blood Antibody Specificity Biomarkers, Tumor - blood Heterophile antibody Hook effect Humans Immunoassay Luminescent Measurements Multiplex suspension bead array Neoplasms - blood Neoplasms - diagnosis Sensitivity and Specificity Tumor marker |
| title | Improvement for identification of heterophile antibody interference and AFP hook effect in immunoassays with multiplex suspension bead array system |
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