Determination of vitamin D in fortified and nonfortified milk powder and infant formula using a specific radioassay after purification by high-performance liquid chromatography
A reliable and sensitive radioassay is described for the determination of vitamin D in milk powder and infant formula. After saponification of the sample interfering compounds like sterols are removed by digitonin precipitation and chromatography on small columns packed with alumina. ( super(3)H)Vit...
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| Veröffentlicht in: | J. Agric. Food Chem.; (United States) 1986-03, Vol.34 (2), p.264-268 |
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| container_title | J. Agric. Food Chem.; (United States) |
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| creator | Van den Berg, Henk Boshuis, Peter G Schreurs, Wil H. P |
| description | A reliable and sensitive radioassay is described for the determination of vitamin D in milk powder and infant formula. After saponification of the sample interfering compounds like sterols are removed by digitonin precipitation and chromatography on small columns packed with alumina. ( super(3)H)Vitamin D is added to the sample as an internal standard, to correct for losses due to previtamin D formation, as well as other procedural losses. Vitamin D is quantitated by a competitive protein binding (CPB) assay after final cleanup of the extract on a straight-phase HPLC column. Diluted sheep serum is used as the source of the binding protein, having equal affinity for both vitamins D sub(2) and D sub(3). |
| doi_str_mv | 10.1021/jf00068a028 |
| format | Article |
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Psychology ; INFANT FOODS ; INFANTS ; ISOTOPE APPLICATIONS ; LABELLED COMPOUNDS ; LAIT DESHYDRATE ; LECHE EN POLVO ; MATERIALS ; MEASURING METHODS ; METHODE ; METHODS ; METODOS ; MILK ; Milk and cheese industries. Ice creams ; PURIFICACION ; PURIFICATION ; RADIOASSAY ; SEPARATION PROCESSES ; TRACER TECHNIQUES ; TRITIUM COMPOUNDS ; VITAMIN D ; VITAMINA D ; VITAMINE D ; VITAMINS</subject><ispartof>J. Agric. 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P</creatorcontrib><creatorcontrib>Institute CIVO-Toxicology and Nutrition TNO, AF Zeist, Netherlands</creatorcontrib><title>Determination of vitamin D in fortified and nonfortified milk powder and infant formula using a specific radioassay after purification by high-performance liquid chromatography</title><title>J. Agric. Food Chem.; (United States)</title><addtitle>J. Agric. Food Chem</addtitle><description>A reliable and sensitive radioassay is described for the determination of vitamin D in milk powder and infant formula. After saponification of the sample interfering compounds like sterols are removed by digitonin precipitation and chromatography on small columns packed with alumina. ( super(3)H)Vitamin D is added to the sample as an internal standard, to correct for losses due to previtamin D formation, as well as other procedural losses. Vitamin D is quantitated by a competitive protein binding (CPB) assay after final cleanup of the extract on a straight-phase HPLC column. Diluted sheep serum is used as the source of the binding protein, having equal affinity for both vitamins D sub(2) and D sub(3).</description><subject>553001 - Agriculture & Food Technology- Tracer Techniques- (-1987)</subject><subject>60 APPLIED LIFE SCIENCES</subject><subject>AGE GROUPS</subject><subject>ALIMENT POUR NOURRISSON</subject><subject>ALIMENTOS PARA NINOS</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL MATERIALS</subject><subject>BODY FLUIDS</subject><subject>CHILDREN</subject><subject>CHROMATOGRAPHIE</subject><subject>CHROMATOGRAPHY</subject><subject>CPB</subject><subject>CROMATOGRAFIA</subject><subject>DRIED MILK</subject><subject>EPURATION</subject><subject>FOOD</subject><subject>Food industries</subject><subject>FORTIFICATION</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>INFANT FOODS</subject><subject>INFANTS</subject><subject>ISOTOPE APPLICATIONS</subject><subject>LABELLED COMPOUNDS</subject><subject>LAIT DESHYDRATE</subject><subject>LECHE EN POLVO</subject><subject>MATERIALS</subject><subject>MEASURING METHODS</subject><subject>METHODE</subject><subject>METHODS</subject><subject>METODOS</subject><subject>MILK</subject><subject>Milk and cheese industries. Ice creams</subject><subject>PURIFICACION</subject><subject>PURIFICATION</subject><subject>RADIOASSAY</subject><subject>SEPARATION PROCESSES</subject><subject>TRACER TECHNIQUES</subject><subject>TRITIUM COMPOUNDS</subject><subject>VITAMIN D</subject><subject>VITAMINA D</subject><subject>VITAMINE D</subject><subject>VITAMINS</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><recordid>eNptkU1v1DAQhiMEEkvhxI2ThRAcUMBOYsd7RC3lo0Ug7XK2Zp3xrreJndoOdP8VPxEvqQoHLh553mfeGc0UxVNG3zBasbd7QykVEmgl7xULxitacsbk_WJBs1xKLtjD4lGM-4xJ3tJF8esME4bBOkjWO-IN-WET5D85I_kxPiRrLHYEXEecd38Tg-2vyOh_dhj-iNYZcOlYMUw9kClatyVA4og6F2gSoLMeYoQDAZN7knEKR2FuvDmQnd3uyhHD0QGcRtLb68l2RO-CHyD5bYBxd3hcPDDQR3xyG0-K9fn79enH8vLrh0-n7y5LaGibyhqgXfK2pRWwSovNpjXIu7qmdUVRYtPImlcdMkFb5BLR0E42grdLKUTO1CfF89nWx2RV1Dah3mnvHOqkeCuEZCJDL2doDP56wpjUYKPGvgeHfoqKNZyLZbXM4OsZ1MHHGNCoMdgBwkExqo6XU_9cLtMvbm0hauhNyNuw8a5EiqphnGasnDEbE97cyRCulGjrlqv1t5WiX1YX_OJzpZrMP5t5A17BNmTL7yspGsrlccJXswg6qr2fgsvL_e90vwEL4787</recordid><startdate>19860301</startdate><enddate>19860301</enddate><creator>Van den Berg, Henk</creator><creator>Boshuis, Peter G</creator><creator>Schreurs, Wil H. P</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>OTOTI</scope></search><sort><creationdate>19860301</creationdate><title>Determination of vitamin D in fortified and nonfortified milk powder and infant formula using a specific radioassay after purification by high-performance liquid chromatography</title><author>Van den Berg, Henk ; Boshuis, Peter G ; Schreurs, Wil H. P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a407t-3aa7957702a12c6bb7fe5d330320e8e448352de1607e58eef0d8465798667e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>553001 - Agriculture & Food Technology- Tracer Techniques- (-1987)</topic><topic>60 APPLIED LIFE SCIENCES</topic><topic>AGE GROUPS</topic><topic>ALIMENT POUR NOURRISSON</topic><topic>ALIMENTOS PARA NINOS</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL MATERIALS</topic><topic>BODY FLUIDS</topic><topic>CHILDREN</topic><topic>CHROMATOGRAPHIE</topic><topic>CHROMATOGRAPHY</topic><topic>CPB</topic><topic>CROMATOGRAFIA</topic><topic>DRIED MILK</topic><topic>EPURATION</topic><topic>FOOD</topic><topic>Food industries</topic><topic>FORTIFICATION</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>INFANT FOODS</topic><topic>INFANTS</topic><topic>ISOTOPE APPLICATIONS</topic><topic>LABELLED COMPOUNDS</topic><topic>LAIT DESHYDRATE</topic><topic>LECHE EN POLVO</topic><topic>MATERIALS</topic><topic>MEASURING METHODS</topic><topic>METHODE</topic><topic>METHODS</topic><topic>METODOS</topic><topic>MILK</topic><topic>Milk and cheese industries. Ice creams</topic><topic>PURIFICACION</topic><topic>PURIFICATION</topic><topic>RADIOASSAY</topic><topic>SEPARATION PROCESSES</topic><topic>TRACER TECHNIQUES</topic><topic>TRITIUM COMPOUNDS</topic><topic>VITAMIN D</topic><topic>VITAMINA D</topic><topic>VITAMINE D</topic><topic>VITAMINS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Van den Berg, Henk</creatorcontrib><creatorcontrib>Boshuis, Peter G</creatorcontrib><creatorcontrib>Schreurs, Wil H. P</creatorcontrib><creatorcontrib>Institute CIVO-Toxicology and Nutrition TNO, AF Zeist, Netherlands</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>OSTI.GOV</collection><jtitle>J. Agric. Food Chem.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Van den Berg, Henk</au><au>Boshuis, Peter G</au><au>Schreurs, Wil H. P</au><aucorp>Institute CIVO-Toxicology and Nutrition TNO, AF Zeist, Netherlands</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of vitamin D in fortified and nonfortified milk powder and infant formula using a specific radioassay after purification by high-performance liquid chromatography</atitle><jtitle>J. Agric. Food Chem.; (United States)</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>1986-03-01</date><risdate>1986</risdate><volume>34</volume><issue>2</issue><spage>264</spage><epage>268</epage><pages>264-268</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>A reliable and sensitive radioassay is described for the determination of vitamin D in milk powder and infant formula. After saponification of the sample interfering compounds like sterols are removed by digitonin precipitation and chromatography on small columns packed with alumina. ( super(3)H)Vitamin D is added to the sample as an internal standard, to correct for losses due to previtamin D formation, as well as other procedural losses. Vitamin D is quantitated by a competitive protein binding (CPB) assay after final cleanup of the extract on a straight-phase HPLC column. Diluted sheep serum is used as the source of the binding protein, having equal affinity for both vitamins D sub(2) and D sub(3).</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><doi>10.1021/jf00068a028</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-8561 |
| ispartof | J. Agric. Food Chem.; (United States), 1986-03, Vol.34 (2), p.264-268 |
| issn | 0021-8561 1520-5118 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_14556929 |
| source | ACS Publications |
| subjects | 553001 - Agriculture & Food Technology- Tracer Techniques- (-1987) 60 APPLIED LIFE SCIENCES AGE GROUPS ALIMENT POUR NOURRISSON ALIMENTOS PARA NINOS Biological and medical sciences BIOLOGICAL MATERIALS BODY FLUIDS CHILDREN CHROMATOGRAPHIE CHROMATOGRAPHY CPB CROMATOGRAFIA DRIED MILK EPURATION FOOD Food industries FORTIFICATION Fundamental and applied biological sciences. Psychology INFANT FOODS INFANTS ISOTOPE APPLICATIONS LABELLED COMPOUNDS LAIT DESHYDRATE LECHE EN POLVO MATERIALS MEASURING METHODS METHODE METHODS METODOS MILK Milk and cheese industries. Ice creams PURIFICACION PURIFICATION RADIOASSAY SEPARATION PROCESSES TRACER TECHNIQUES TRITIUM COMPOUNDS VITAMIN D VITAMINA D VITAMINE D VITAMINS |
| title | Determination of vitamin D in fortified and nonfortified milk powder and infant formula using a specific radioassay after purification by high-performance liquid chromatography |
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