Analysis of Plasmodium falciparum growth in culture using acridine orange and flow cytometry

The growth of Plasmodium falciparum in cultures of human red blood cells was studied using acridine orange to stain RNA and DNA, followed by flow cytometric analysis. The cycle of the parasite is characterized by a period of growth, prior to initiation of DNA synthesis, in which a significant increa...

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Veröffentlicht in:	The journal of histochemistry and cytochemistry 1986-02, Vol.34 (2), p.215-220
Hauptverfasser:	Hare, JD, Bahler, DW
Format:	Artikel
Sprache:	eng
Schlagworte:	Acridine Orange Animals Aphidicolin Biological and medical sciences Diterpenes - pharmacology DNA - analysis Flow Cytometry Fundamental and applied biological sciences. Psychology Humans Life cycle. Host-agent relationship. Pathogenesis Mannitol - pharmacology Plasmodium falciparum Plasmodium falciparum - cytology Plasmodium falciparum - growth & development Protozoa RNA - analysis Staining and Labeling
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