Studies of Macrostome Formation of Low-Transforming Tetrahymena vorax. Transformation Enhancers, Generation Time, and Membrane Fluidity

Low-transforming populations of Tetrahymena vorax are known to arise spontaneously in subculture and may retain the low-transforming characteristic for several years. In this paper, the authors present evidence that iron, copper, retinol palmitate (synthetic vitamin A), and elevated hydrogen ion con...

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Veröffentlicht in:The Journal of Protozoology 1986-08, Vol.33 (3), p.382-387
Hauptverfasser: RYALS, PHILLIP E., SMITH-SOMERVILLE, HARRIETT E.
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container_title The Journal of Protozoology
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creator RYALS, PHILLIP E.
SMITH-SOMERVILLE, HARRIETT E.
description Low-transforming populations of Tetrahymena vorax are known to arise spontaneously in subculture and may retain the low-transforming characteristic for several years. In this paper, the authors present evidence that iron, copper, retinol palmitate (synthetic vitamin A), and elevated hydrogen ion concentration enhance heat-shock-induced macrostome formation and that the time required for maximum transformation is approximately that of the doubling time of the low-transforming population. The effect of transformation-enhancing compounds on cell division and membrane fluidity as inferred by polarization of fluorescence was also investigated.
doi_str_mv 10.1111/j.1550-7408.1986.tb05627.x
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1550-7408
2375-0804
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subjects Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Protozoa
Systematics. Geographical distribution. Morphology. Cytology
Tetrahymena vorax
title Studies of Macrostome Formation of Low-Transforming Tetrahymena vorax. Transformation Enhancers, Generation Time, and Membrane Fluidity
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