Interaction of paraquat and amine uptake by rat lungs perfused in situ
The kinetics of ( super(14)C)paraquat (N,N-di( super(14)C)methyl-4,4'-bipyridylium) uptake from the pulmonary circulation were investigated in rat lungs perfused in situ. During the 1st h of exposure to the herbicide, paraquat entered the lungs primarily by diffusion; no evidence was obtained f...
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Veröffentlicht in: | American Journal of Physiology: Cell Physiology 1985-01, Vol.249 (5), p.E506-E513 |
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container_title | American Journal of Physiology: Cell Physiology |
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creator | Rannels, DE Pegg, A E Clark, R S Addison, J L |
description | The kinetics of ( super(14)C)paraquat (N,N-di( super(14)C)methyl-4,4'-bipyridylium) uptake from the pulmonary circulation were investigated in rat lungs perfused in situ. During the 1st h of exposure to the herbicide, paraquat entered the lungs primarily by diffusion; no evidence was obtained from concentrative uptake, saturation kinetics (1-3,500 mu M paraquat), or inhibition by the amines methylglyoxal bis(guanylhydrazone) or spermidine, both of which were expected to compete for paraquat transport sites. After 60 min of exposure, uptake rates increased two- to three-fold, and paraquate was accumulated to an apparent intracellular concentration greater than that in the perfusate. The latter phase of paraquat uptake was saturable and was inhibited by methylglyoxal bis(guanylhydrazone); it did not appear to reflect a progressive paraquat-induced alteration in cellular permeability but predominance of a rapid, carrier-mediated uptake pathway. |
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During the 1st h of exposure to the herbicide, paraquat entered the lungs primarily by diffusion; no evidence was obtained from concentrative uptake, saturation kinetics (1-3,500 mu M paraquat), or inhibition by the amines methylglyoxal bis(guanylhydrazone) or spermidine, both of which were expected to compete for paraquat transport sites. After 60 min of exposure, uptake rates increased two- to three-fold, and paraquate was accumulated to an apparent intracellular concentration greater than that in the perfusate. The latter phase of paraquat uptake was saturable and was inhibited by methylglyoxal bis(guanylhydrazone); it did not appear to reflect a progressive paraquat-induced alteration in cellular permeability but predominance of a rapid, carrier-mediated uptake pathway.</description><identifier>ISSN: 0363-6143</identifier><language>eng</language><ispartof>American Journal of Physiology: Cell Physiology, 1985-01, Vol.249 (5), p.E506-E513</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>Rannels, DE</creatorcontrib><creatorcontrib>Pegg, A E</creatorcontrib><creatorcontrib>Clark, R S</creatorcontrib><creatorcontrib>Addison, J L</creatorcontrib><title>Interaction of paraquat and amine uptake by rat lungs perfused in situ</title><title>American Journal of Physiology: Cell Physiology</title><description>The kinetics of ( super(14)C)paraquat (N,N-di( super(14)C)methyl-4,4'-bipyridylium) uptake from the pulmonary circulation were investigated in rat lungs perfused in situ. During the 1st h of exposure to the herbicide, paraquat entered the lungs primarily by diffusion; no evidence was obtained from concentrative uptake, saturation kinetics (1-3,500 mu M paraquat), or inhibition by the amines methylglyoxal bis(guanylhydrazone) or spermidine, both of which were expected to compete for paraquat transport sites. After 60 min of exposure, uptake rates increased two- to three-fold, and paraquate was accumulated to an apparent intracellular concentration greater than that in the perfusate. The latter phase of paraquat uptake was saturable and was inhibited by methylglyoxal bis(guanylhydrazone); it did not appear to reflect a progressive paraquat-induced alteration in cellular permeability but predominance of a rapid, carrier-mediated uptake pathway.</description><issn>0363-6143</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><recordid>eNqNyr0KwjAUQOEMCtafd7iTWyFtYtFZLLq7l6u9lWiapLnJ4Nvr4AM4Hfg4M1FI1aiyqbRaiCXzU0qp6-ZQiPbiEkW8J-Md-AECRpwyJkDXA47GEeSQ8EVwe0P8us3uwRAoDpmpB-OATcprMR_QMm1-XYlte7oez2WIfsrEqRsN38ladOQzd5Xe1Vrpvfp7_ADlWj3i</recordid><startdate>19850101</startdate><enddate>19850101</enddate><creator>Rannels, DE</creator><creator>Pegg, A E</creator><creator>Clark, R S</creator><creator>Addison, J L</creator><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>19850101</creationdate><title>Interaction of paraquat and amine uptake by rat lungs perfused in situ</title><author>Rannels, DE ; Pegg, A E ; Clark, R S ; Addison, J L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_145243483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rannels, DE</creatorcontrib><creatorcontrib>Pegg, A E</creatorcontrib><creatorcontrib>Clark, R S</creatorcontrib><creatorcontrib>Addison, J L</creatorcontrib><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>American Journal of Physiology: Cell Physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rannels, DE</au><au>Pegg, A E</au><au>Clark, R S</au><au>Addison, J L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interaction of paraquat and amine uptake by rat lungs perfused in situ</atitle><jtitle>American Journal of Physiology: Cell Physiology</jtitle><date>1985-01-01</date><risdate>1985</risdate><volume>249</volume><issue>5</issue><spage>E506</spage><epage>E513</epage><pages>E506-E513</pages><issn>0363-6143</issn><abstract>The kinetics of ( super(14)C)paraquat (N,N-di( super(14)C)methyl-4,4'-bipyridylium) uptake from the pulmonary circulation were investigated in rat lungs perfused in situ. During the 1st h of exposure to the herbicide, paraquat entered the lungs primarily by diffusion; no evidence was obtained from concentrative uptake, saturation kinetics (1-3,500 mu M paraquat), or inhibition by the amines methylglyoxal bis(guanylhydrazone) or spermidine, both of which were expected to compete for paraquat transport sites. After 60 min of exposure, uptake rates increased two- to three-fold, and paraquate was accumulated to an apparent intracellular concentration greater than that in the perfusate. The latter phase of paraquat uptake was saturable and was inhibited by methylglyoxal bis(guanylhydrazone); it did not appear to reflect a progressive paraquat-induced alteration in cellular permeability but predominance of a rapid, carrier-mediated uptake pathway.</abstract></addata></record> |
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title | Interaction of paraquat and amine uptake by rat lungs perfused in situ |
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