Carboxysomes of Thiobacillus neapolitanus do not contain extrachromosomal DNA
Carboxysomes isolated from Thiobacillus neapolitanus are surrounded by double‐stranded DNA. This DNA is not required for the stability of the carboxysomes, since the carboxysomes remain intact after treatment with DNase I. DNA could not be isolated from these DNAse‐treated particles, indicating that...
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| Veröffentlicht in: | FEMS microbiology letters 1986-07, Vol.35 (2‐3), p.193-198 |
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| container_end_page | 198 |
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| container_issue | 2‐3 |
| container_start_page | 193 |
| container_title | FEMS microbiology letters |
| container_volume | 35 |
| creator | Holthuijzen, Yolande A. Maathuis, Frans J.M. Kuenen, J.Gijs Konings, Ruud N.H. Konings, Wil N. |
| description | Carboxysomes isolated from Thiobacillus neapolitanus are surrounded by double‐stranded DNA. This DNA is not required for the stability of the carboxysomes, since the carboxysomes remain intact after treatment with DNase I. DNA could not be isolated from these DNAse‐treated particles, indicating that no DNA was present inside the carboxysomes. DNA attached to the carboxysomes is heterogeneous in size (4–17 μm) with at least 50 kilobase pairs. Carboxysomal DNA and chromosomal DNA have the same electrophoretic mobility and hybridize completely. Restriction enzyme digestion of both carboxysomal and chromosomal DNA show identical restriction fragment patterns.
These results indicate that the DNA found in the carboxysomal preparations is chromosomal DNA which is attached to the carboxysomes. |
| doi_str_mv | 10.1111/j.1574-6968.1986.tb01526.x |
| format | Article |
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These results indicate that the DNA found in the carboxysomal preparations is chromosomal DNA which is attached to the carboxysomes.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1986.tb01526.x</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Bacteriology ; Biological and medical sciences ; carboxysomes ; Extrachromosomal DNA ; Fundamental and applied biological sciences. Psychology ; Genetics ; Microbiology ; Thiobacillus neapolitanus</subject><ispartof>FEMS microbiology letters, 1986-07, Vol.35 (2‐3), p.193-198</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3763-bd60e426d5c4794de220ea66d74db76bb7f9f239aff9fe8095233d55f9f394003</citedby><cites>FETCH-LOGICAL-c3763-bd60e426d5c4794de220ea66d74db76bb7f9f239aff9fe8095233d55f9f394003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.1986.tb01526.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.1986.tb01526.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7942892$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Holthuijzen, Yolande A.</creatorcontrib><creatorcontrib>Maathuis, Frans J.M.</creatorcontrib><creatorcontrib>Kuenen, J.Gijs</creatorcontrib><creatorcontrib>Konings, Ruud N.H.</creatorcontrib><creatorcontrib>Konings, Wil N.</creatorcontrib><title>Carboxysomes of Thiobacillus neapolitanus do not contain extrachromosomal DNA</title><title>FEMS microbiology letters</title><description>Carboxysomes isolated from Thiobacillus neapolitanus are surrounded by double‐stranded DNA. This DNA is not required for the stability of the carboxysomes, since the carboxysomes remain intact after treatment with DNase I. DNA could not be isolated from these DNAse‐treated particles, indicating that no DNA was present inside the carboxysomes. DNA attached to the carboxysomes is heterogeneous in size (4–17 μm) with at least 50 kilobase pairs. Carboxysomal DNA and chromosomal DNA have the same electrophoretic mobility and hybridize completely. Restriction enzyme digestion of both carboxysomal and chromosomal DNA show identical restriction fragment patterns.
These results indicate that the DNA found in the carboxysomal preparations is chromosomal DNA which is attached to the carboxysomes.</description><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>carboxysomes</subject><subject>Extrachromosomal DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>Microbiology</subject><subject>Thiobacillus neapolitanus</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><recordid>eNqVkF9LwzAUxYMoOKffoYj41po_bdL4IMh0Kmz6Mp9DmqYsI21m0mH37U3Z2Lv35XK555x7-QFwi2CGYj1sMlSwPKWclhniJc36CqIC02w4A5PT6hxMIGFliiBnl-AqhA2EMMeQTsByJn3lhn1wrQ6Ja5LV2rhKKmPtLiSdlltnTS-7ONQu6VyfKNf10nSJHnov1dq71kWztMnL5_M1uGikDfrm2Kfge_66mr2ni6-3j9nzIlWEUZJWNYU6x7QuVM54XmuMoZaU1iyvK0arijW8wYTLJnZdQl5gQuqiiBPhOYRkCu4PuVvvfnY69KI1QWlrZafdLgiUFxiVrIjCx4NQeReC143YetNKvxcIipGg2IgRkxgxiZGgOBIUQzTfHa_IoKRtvOyUCaeE-DkuOY6yp4Ps11i9_8cBMV8uECfkD0aBhoc</recordid><startdate>198607</startdate><enddate>198607</enddate><creator>Holthuijzen, Yolande A.</creator><creator>Maathuis, Frans J.M.</creator><creator>Kuenen, J.Gijs</creator><creator>Konings, Ruud N.H.</creator><creator>Konings, Wil N.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>198607</creationdate><title>Carboxysomes of Thiobacillus neapolitanus do not contain extrachromosomal DNA</title><author>Holthuijzen, Yolande A. ; Maathuis, Frans J.M. ; Kuenen, J.Gijs ; Konings, Ruud N.H. ; Konings, Wil N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3763-bd60e426d5c4794de220ea66d74db76bb7f9f239aff9fe8095233d55f9f394003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>carboxysomes</topic><topic>Extrachromosomal DNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>Microbiology</topic><topic>Thiobacillus neapolitanus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Holthuijzen, Yolande A.</creatorcontrib><creatorcontrib>Maathuis, Frans J.M.</creatorcontrib><creatorcontrib>Kuenen, J.Gijs</creatorcontrib><creatorcontrib>Konings, Ruud N.H.</creatorcontrib><creatorcontrib>Konings, Wil N.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Holthuijzen, Yolande A.</au><au>Maathuis, Frans J.M.</au><au>Kuenen, J.Gijs</au><au>Konings, Ruud N.H.</au><au>Konings, Wil N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Carboxysomes of Thiobacillus neapolitanus do not contain extrachromosomal DNA</atitle><jtitle>FEMS microbiology letters</jtitle><date>1986-07</date><risdate>1986</risdate><volume>35</volume><issue>2‐3</issue><spage>193</spage><epage>198</epage><pages>193-198</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Carboxysomes isolated from Thiobacillus neapolitanus are surrounded by double‐stranded DNA. This DNA is not required for the stability of the carboxysomes, since the carboxysomes remain intact after treatment with DNase I. DNA could not be isolated from these DNAse‐treated particles, indicating that no DNA was present inside the carboxysomes. DNA attached to the carboxysomes is heterogeneous in size (4–17 μm) with at least 50 kilobase pairs. Carboxysomal DNA and chromosomal DNA have the same electrophoretic mobility and hybridize completely. Restriction enzyme digestion of both carboxysomal and chromosomal DNA show identical restriction fragment patterns.
These results indicate that the DNA found in the carboxysomal preparations is chromosomal DNA which is attached to the carboxysomes.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1574-6968.1986.tb01526.x</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1986-07, Vol.35 (2‐3), p.193-198 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_14521875 |
| source | Oxford University Press Archive; Access via Wiley Online Library; Alma/SFX Local Collection |
| subjects | Bacteriology Biological and medical sciences carboxysomes Extrachromosomal DNA Fundamental and applied biological sciences. Psychology Genetics Microbiology Thiobacillus neapolitanus |
| title | Carboxysomes of Thiobacillus neapolitanus do not contain extrachromosomal DNA |
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